In focus in HCB

Taatjes, Douglas J.; Roth, Jürgen

doi:10.1007/s00418-021-02044-2

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…In conclusion, the antibody used here has been rigorously tested and validated for specificity in a variety of applications, which is essential for a correct interpretation of the results. Indeed, cross-reactivity of antibodies with unwanted proteins is one of the main causes of the lack of reproducibility between studies ( Saper, 2005 ; Voskuil, 2014 ; Taatjes and Roth, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Up-regulation of CB1 cannabinoid receptors located at glutamatergic terminals in the medial prefrontal cortex of the obese Zucker rat

Echeazarra

Barrondo²,

Caño³

et al. 2022

Front. Neuroanat.

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The present study describes a detailed neuroanatomical distribution map of the cannabinoid type 1 (CB1) receptor, along with the biochemical characterization of the expression and functional coupling to their cognate Gi/o proteins in the medial prefrontal cortex (mPCx) of the obese Zucker rats. The CB1 receptor density was higher in the prelimbic (PL) and infralimbic (IL) subregions of the mPCx of obese Zucker rats relative to their lean littermates which was associated with a higher percentage of CB1 receptor immunopositive excitatory presynaptic terminals in PL and IL. Also, a higher expression of CB1 receptors and WIN55,212-2-stimulated [35S]GTPγS binding was observed in the mPCx but not in the neocortex (NCx) and hippocampus of obese rats. Low-frequency stimulation in layers II/III of the mPCx induced CB1 receptor-dependent long-term synaptic plasticity in IL of area obese Zucker but not lean rats. Overall, the elevated 2-AG levels, up-regulation of CB1 receptors, and increased agonist-stimulated [35S]GTPγS binding strongly suggest that hyperactivity of the endocannabinoid signaling takes place at the glutamatergic terminals of the mPCx in the obese Zucker rat. These findings could endorse the importance of the CB1 receptors located in the mPCx in the development of obesity in Zucker rats.

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Section: Discussionmentioning

confidence: 99%