In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53

Deneka, Magdalena; Pelchen–Matthews, Annegret; Byland, Rahel; Ruiz‐Mateos, Ezequiel; Marsh, Mark

doi:10.1084/jem2045oia13

Cited by 52 publications

(121 citation statements)

References 19 publications

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“…For immuno-fluorescence, cells seeded on coverslips were transfected as above. After 48 h cells were fixed, quenched, blocked as described (32), and permeabilized with 0.1% TX-100. Primary antibodies used were: rat FITC anti-HA antibody (Roche), mouse anti-Xpress IgG1 (Invitrogen), and mouse anti-p17 antibody 4C9 ARP342 (Centre for AIDS Reagents, NIBSC, Potters Bar, UK).…”

Section: Methodsmentioning

confidence: 99%

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Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration

Mlčochová²,

Pelchen–Matthews³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Tetherin is an IFN-inducible restriction factor that inhibits HIV-1 particle release in the absence of the HIV-1 countermeasure, viral protein U (Vpu). Although ubiquitous in HIV-1 and simian immunodeficiency viruses from chimpanzees, greater spot nosed monkeys, mustached monkeys, and Mona monkeys, other primate lentiviruses do not encode a Vpu protein. Here we demonstrate that SIV from Tantalus monkeys (SIVtan) encodes an envelope glycoprotein (SIVtan Env) able to counteract tetherin from Tantalus monkeys, rhesus monkeys, sooty mangabeys, and humans, but not from pigs. We show that sensitivity to Vpu but not SIVtan Env can be transferred with the human tetherin transmembrane region. We also identify a mutation in the tetherin extracellular domain, which almost completely abolishes sensitivity of human tetherin to SIVtan Env without compromising antiviral activity or sensitivity to Vpu. SIVtan Env expression results in a reduction of surface tetherin, as well as reduction in tetherin co-localization with mature surface-associated virus. Immuno-electron microscopy reveals co-localization of SIVtan Env with tetherin in intracellular tubulo-vesicular structures, suggesting that tetherin is sequestered away from budding virions at the cell surface. Along with HIV-1 Vpu and SIV Nef, envelope glycoprotein is the third and most broadly active lentiviral-encoded tetherin countermeasure to be described. Our observations emphasize the importance of tetherin in protecting mammals against viral infection and suggest that HIV-1 Vpu inhibitors may select active envelope mutants.HIV ͉ restriction ͉ innate immunity

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Section: Methodsmentioning

confidence: 99%

“…Transfected 293T cells were fixed, embedded, and frozen for cryosectioning as described (32). Ultrathin (50-to 60-nm) cryosections were stained with anti-tetherin (MaxPab, Abnova) or mouse anti-HA (Covance), a rabbit anti-mouse bridging antibody (DakoCytomation), and 10 nm protein A-gold (PAG, the EM Lab, Utrecht University, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration

Mlčochová²,

Pelchen–Matthews³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

150

185

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“…Macrophages are among the first cells infected by HIV. They are thought to serve as a reservoir, protecting HIV from neutralizing antibodies, and to bud HIV-1 primarily at the plasma membrane [28,29]. Macrophages can then transfer the virus during activation of other immune cells.…”

Section: B Macrophagesmentioning

confidence: 99%

Oxidants and Antioxidants in the Pathogenesis of HIV/AIDS

Agarwal¹

2011

TORSJ

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Human immunodeficiency virus (HIV) has predominantly been considered the main cause in the progression of acquired immunodeficiency syndrome (AIDS). However, many researchers believe that there are co-factors involved along the way that may play a critical role in its development. Elevated levels of reactive oxygen species (ROS) have been established to be present at the onset of infection. Additionally, the delayed response by the immune system upon infection may be due to an initial depletion of antioxidants, which play a critical role in scavenging excess ROS to maintain normal physiological conditions. This pro-oxidant/antioxidant imbalance results in a condition known as oxidative stress (OS). OS has been reported to be an integral element in the progression of many diseases, including AIDS. Therefore, it is presumed that antioxidant treatment may provide a promising and cost-effective therapeutic approach in treating HIV-infected individuals on a global scale.Although HIV infection presents one of the most arduous complications to humans worldwide, awareness of its origin, adverse effects, and potential treatment remain confined to a limited population. The aim of this article is to inform the reader of how AIDS developed into a global epidemic so rapidly, while emphasizing the relationship between oxidants and antioxidants in the pathogenesis of HIV/AIDS. A basic review of reduction/oxidation (redox) reactions is provided to understand how such simple introductory concepts can have such profound effects on the body. This serves as an essential building block to the topics that follow: ROS, antioxidants, and OS in HIV disease. Moreover, it allows for us to better define and elucidate OS as an often-overlooked link between HIV-infection and the progression of AIDS, as well as potential antioxidant treatment.

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“…Plus récemment, il a été suggéré que les compartiments intracellulaires observés pourraient correspondre en fait à des domaines de la membrane plasmique formant de profondes invaginations dans la cellule [31,32]. L'implication de la machinerie de biogenèse des MVB milite évidemment pour un bourgeonnement endosomique.…”

Section: Une Variété De Sites Possibles De Bourgeonnementunclassified

Une nouvelle vision de l’assemblage du VIH-1

et al. 2008

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Le rôle pilote de la polyprotéine GagLes particules rétrovirales sont constituées d'une enveloppe lipidique où sont ancrées les glycoprotéines virales Env, contenant le coeur viral. Celui-ci est composé de protéines et du génome viral constitué de deux molécules d'ARN simple-brin identiques appariées par des liaisons non-covalentes. Les protéines du coeur, dont la nature et la fonction sont décrites dans la Figure 1, sont produites par traduction de l'ARN génomique viral (ARNg) par des mécanismes complexes dont certains dépendent de sites d'entrée interne des ribosomes (IRES) [1], pour donner deux précurseurs polyprotéiques : Gag regroupe les protéines organisant la structure de la particule, et Gag-Pol contient les protéines à activité enzymatique (protéase, transcriptase inverse et intégrase), en plus des précéden-tes. Dans la plupart des rétrovirus, dont le VIH-1, l'assemblage du coeur se fait de façon concomitante au bourgeonnement viral et à la maturation protéolytique de Gag et Gag-Pol par la protéase virale, pour donner un virion enveloppé contenant les protéines matures (Figure 1).

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In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53

Cited by 52 publications

References 19 publications

Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration

Simian immunodeficiency virus envelope glycoprotein counteracts tetherin/BST-2/CD317 by intracellular sequestration

Oxidants and Antioxidants in the Pathogenesis of HIV/AIDS

Une nouvelle vision de l’assemblage du VIH-1

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