In ovaries with high or low variation in follicle size, granulosa cells of antral follicles exhibit distinct size-related processes

Costermans, N.G.J.; Keijer, Jaap; Schothorst, Evert M. van; Kemp, B.; Keshtkar, Shohreh; Bunschoten, Annelies; Soede, N.M.; Teerds, K. J.

doi:10.1093/molehr/gaz042

Cited by 12 publications

(9 citation statements)

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“…Smaller follicles were more proliferative, as shown by a higher expression of genes involved in cell cycle progression and protein translation, while larger follicles were more differentiated as shown by a higher expression of follicular maturation markers. Different from Costermans et al, 2019a; in the current analysis follicle size was similar in high versus low weight loss sows, and observed effects are therefore due to lactational weight loss. These results indicate that besides described effects of lactational weight loss on follicle size, weight loss also sustains granulosa cell proliferation which may delay granulosa cell differentiation, independent of follicle size.…”

Section: Introductioncontrasting

confidence: 94%

“…Together, this may suggest that granulosa cells of sows with a high versus low weight loss during lactation are more proliferative. To assess this, protein expression of Ki‐67, a proliferation marker (Gerdes et al, 1984), was compared for granulosa cells of sows with high versus low weight loss during lactation (see Costermans et al, 2019a for experimental details). Ki‐67 protein expression tended to be higher ( p = 0.09) for sows with a high versus low weight loss during lactation.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, at the protein level, granulosa cell proliferation tended to be higher in sows with a higher weight loss during lactation. In Costermans et al (2019a), larger and smaller follicles in the follicle pool of the 10 largest healthy follicles were compared for sows with variable weight loss. Smaller follicles were more proliferative, as shown by a higher expression of genes involved in cell cycle progression and protein translation, while larger follicles were more differentiated as shown by a higher expression of follicular maturation markers.…”

Section: Introductionmentioning

confidence: 99%

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Physiological and metabolic aspects of follicular developmental competence as affected by lactational body condition loss

Costermans

Teerds

Kemp

et al. 2022

Molecular Reproduction Devel

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Metabolic demands of modern hybrid sows have increased over the years, which increases the chance that sows enter a substantial negative energy balance (NEB) during lactation. This NEB can negatively impact reproductive outcome, which is especially evident in primiparous sows causing a reduced second parity reproductive performance. The negative effects of the lactational NEB on reproductive performance can be partly explained by the influence of the premating metabolic state, during and after lactation, on the development of follicles from which oocytes will give rise to the next litter. In addition, the degree and type of body tissue mobilization during lactation that is, adipose tissue or lean mass, highly influences follicular development. Research investigating relations between the premating metabolic state and follicular and oocyte competence in modern hybrid sows, which experience higher metabolic demands during lactation, is limited. In this review we summarize current knowledge of physiological relations between the metabolic state of modern hybrid sows and follicular developmental competence. In addition, we discuss potential implications of these relations for current sow management strategies.

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Section: Introductioncontrasting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiological and metabolic aspects of follicular developmental competence as affected by lactational body condition loss

Costermans

Teerds

Kemp

et al. 2022

Molecular Reproduction Devel

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“…The relative transcript abundance of INHBA was lower in CCs derived from SF than those from MF, whereas the transcript abundance of FSHR, FST, LHR and NR2F6 was higher in CCs derived from SF. A previous study that analysed the transcriptomic profile of granulosa mural cells derived from SF and MF found no significant effect of follicle diameter for some of the genes analysed (LHR, FST, FSHR, INHBA and VEGFA) in the present study (Costermans et al., 2019).…”

Section: Discussioncontrasting

confidence: 59%

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Moros-Nicolás

Izquierdo-Rico

et al. 2020

Reprod Domestic Animals

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Crosstalk between the oocyte and surrounding cumulus cells (CCs) is essential for the production of competent oocytes. Previous studies have analysed the relative transcript abundance in oocytes derived from small (SF: <3 mm diameter)‐ and medium‐sized (MF: 3–6 mm diameter) follicles to determine the potential use of SF‐derived oocytes in assisted reproductive technologies (ART). The aim of this study was to examine the relative transcript abundance of CCs obtained from cumulus–oocyte complexes (COCs) derived from SF and MF. Nine genes were selected according to their importance for developmental competence: AT‐rich interaction domain 1B (ARID1B), bone morphogenic protein receptor 2 (BMPR2), CD44, follicle‐stimulating hormone receptor (FSHR), follistatin (FST), inhibin beta‐A (INHBA), luteinizing hormone receptor (LHR), nuclear receptor subfamily 2 group F member 6 (NR2F6) and vascular endothelial growth factor A (VEGFA). The expression of these genes was analysed by RT‐qPCR. The results pointed to significant differences in five genes, and the relative transcript abundance of SF‐derived CCs was lower in the case of INHBA, but higher in FSHR, FST, LHR and NR2F6 compared with MF‐derived CCs. We provide information of gene activity in the porcine CCs from different sized follicles, thus improving our understanding of oocyte biology and providing new markers that identify viable and competent oocytes.

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“…Insulin-like growth factor 1 (IGF1) and FSH signals are two important growth factors for folliculogenesis and increase with follicle maturation ( 9 , 10 ). Studies in pigs have shown that direct exposure to high concentrations of glucocorticoids decreases ovarian IGF1 production both in vitro ( 11 ) and in vivo ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Androgens improve ovarian follicle function impaired by glucocorticoids through an androgen-IGF1-FSH synergistic effect

Gao

Gao²,

Wang³

2022

Front. Endocrinol.

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High concentrations of glucocorticoids caused by chronic stress are known to affect ovarian function and cause diminished ovarian reserve. Androgens are essential for early-stage ovarian follicle development, but the effects and mechanisms of androgens on follicle development under chronic stress remain unclear. In this study, we aim to investigate the effects of high concentrations of glucocorticoids on the function of in vitro cultured ovarian cells and mouse early-stage ovarian follicles and to validate the hypothesis that androgen–insulin-like growth factor 1 (IGF1)–follicle-stimulating hormone (FSH) synergistic signaling helps to ameliorate the damage caused by high concentrations of glucocorticoids. KGN cells (human granulosa cell line) and mouse primary cells were treated with different concentrations of glucocorticoids, and the cell proliferation, apoptosis, and sex hormone secretion were detected. The effects of glucocorticoid and androgens on IGF1 receptor (IGF1R) and FSH receptor (FSHR) expression in KGN cells were detected by Western blot. Steroidogenic synthase expressions under androgens and androgen-IGF1-FSH combination treatment were examined by qPCR after manipulation using low and high concentrations of glucocorticoids. The mechanism of androgen regulation of IGF1R and FSHR was explored by small interfering RNA (siRNA) and chromatin immunoprecipitation (ChIP)-qPCR. Damage of glucocorticoids and the treatment effects of androgens were further validated in mouse ovarian follicles cultured in vitro. The results demonstrated that prolonged treatment with high-dose glucocorticoids reduced cell viability of granulosa cells, inhibited their sex hormone secretion, and impaired their sensitivity to IGF1 and FSH signaling by affecting IGF1R and FSHR functions. Androgens at an appropriate dose range improved early-stage follicle development and their hormone secretion under high-dose glucocorticoid treatment, which was related to increased transcription of Igf1r and Fshr. This work showed that excessive glucocorticoids impaired ovarian function and validated that balanced concentrations of androgens synergized with IGF1 and FSH to improve the function of early-stage ovarian follicles under conditions of chronic stress.

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In ovaries with high or low variation in follicle size, granulosa cells of antral follicles exhibit distinct size-related processes

Cited by 12 publications

References 50 publications

Physiological and metabolic aspects of follicular developmental competence as affected by lactational body condition loss

Physiological and metabolic aspects of follicular developmental competence as affected by lactational body condition loss

Relative transcript abundance in porcine cumulus cells collected from different sized follicles

Androgens improve ovarian follicle function impaired by glucocorticoids through an androgen-IGF1-FSH synergistic effect

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