In Search of Pathogens: Transcriptome-Based Identification of Viral Sequences from the Pine Processionary Moth  (Thaumetopoea pityocampa)

Jakubowska, Agata; Nalçacıoğlu, Remziye; Millán-Leiva, Anabel; Sanz-Carbonell, Alejandro; Muratoglu, Hacer; Herrero, Salvador; Demirbağ, Zihni

doi:10.3390/v7020456

Cited by 19 publications

(15 citation statements)

References 69 publications

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“…Co-infection of iflavirus with baculoviruses in field conditions has been reported recently ( Virto et al, 2014 ). However, as the number of novel RNA viruses, including iflaviruses, increases rapidly through the use of massively parallel sequencing methods ( Liu, Vijayendran & Bonning, 2011 ; Jakubowska et al, 2015 ; Carrillo-Tripp et al, 2014 ; Geng et al, 2014 ), the discovery of novel interactions in natural virus populations is likely to grow accordingly. Our results suggest that virus–virus interactions may be more common than currently recognized, and may be influential in the ecology of baculovirus and host populations.…”

Section: Discussionmentioning

confidence: 99%

Iflavirus increases its infectivity and physical stability in association with baculovirus

Jakubowska

Murillo

Carballo

et al. 2016

PeerJ

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Virus transmission and the prevalence of infection depend on multiple factors, including the interaction with other viral pathogens infecting the same host. In this study, active replication of an iflavirus, Spodoptera exigua iflavirus 1 (order Picornavirales) was observed in the offspring of insects that survived following inoculation with a pathogenic baculovirus, Spodoptera exigua multiple nucleopolyhedrovirus. Tracking the origin of the iflavirus suggested the association of this virus with the occlusion bodies of the baculovirus. Here we investigated the effect of this association on the stability and infectivity of both viruses. A reduction in baculovirus pathogenicity, without affecting its infectivity and productivity, was observed when associated with the iflavirus. In contrast, viral association increased the infectivity of the iflavirus and its resistance to ultraviolet radiation and high temperature, two of the main factors affecting virus stability in the field. In addition, electron microscopy analysis revealed the presence of particles resembling iflavirus virions inside the occlusion bodies of the baculovirus, suggesting the possible co-occlusion of both viruses. Results reported here are indicative of facultative phoresis of a virus and suggest that virus–virus interactions may be more common than currently recognized, and may be influential in the ecology of baculovirus and host populations and in consequence in the use of baculoviruses as biological insecticides.

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Section: Discussionmentioning

confidence: 99%

Iflavirus increases its infectivity and physical stability in association with baculovirus

Jakubowska

Murillo

Carballo

et al. 2016

PeerJ

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“…Although incomplete, this reference transcriptome was already useful to compare sets of transcripts between two allochronic populations. New resources were later released to identify associated viral sequences, but most probably corresponded to the sister species T. wilkinsoni (Jakubowska et al, 2015). Recent advances in high-throughput sequencing technologies now allow to obtain valuable resources for nonmodel organisms and to fill the gap between genomic and phenotypic evolution (Mueller, Kuhl, Timmermann, & Kempenaers, 2016).…”

Section: Introductionmentioning

confidence: 99%

Draft genome and reference transcriptomic resources for the urticating pine defoliator Thaumetopoea pityocampa (Lepidoptera: Notodontidae)

Gschloessl

Dorkeld

Bergès³

et al. 2018

Molecular Ecology Resources

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The pine processionary moth Thaumetopoea pityocampa (Lepidoptera: Notodontidae) is the main pine defoliator in the Mediterranean region. Its urticating larvae cause severe human and animal health concerns in the invaded areas. This species shows a high phenotypic variability for various traits, such as phenology, fecundity and tolerance to extreme temperatures. This study presents the construction and analysis of extensive genomic and transcriptomic resources, which are an obligate prerequisite to understand their underlying genetic architecture. Using a well-studied population from Portugal with peculiar phenological characteristics, the karyotype was first determined and a first draft genome of 537 Mb total length was assembled into 68,292 scaffolds (N50 = 164 kb). From this genome assembly, 29,415 coding genes were predicted. To circumvent some limitations for fine-scale physical mapping of genomic regions of interest, a 3X coverage BAC library was also developed. In particular, 11 BACs from this library were individually sequenced to assess the assembly quality. Additionally, de novo transcriptomic resources were generated from various developmental stages sequenced with HiSeq and MiSeq Illumina technologies. The reads were de novo assembled into 62,376 and 63,175 transcripts, respectively. Then, a robust subset of the genome-predicted coding genes, the de novo transcriptome assemblies and previously published 454/Sanger data were clustered to obtain a high-quality and comprehensive reference transcriptome consisting of 29,701 bona fide unigenes. These sequences covered 99% of the cegma and 88% of the busco highly conserved eukaryotic genes and 84% of the busco arthropod gene set. Moreover, 90% of these transcripts could be localized on the draft genome. The described information is available via a genome annotation portal (http://bipaa.genouest.org/sp/thaumetopoea_pityocampa/).

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“…Coinfection of iflavirus with baculoviruses in field conditions has been reported recently (Virto et al, 2014). However, as the number of novel RNA viruses, including iflaviruses, increases rapidly through the use of massively parallel sequencing methods (Liu, Vijayendran & Bonning, 2011;Jakubowska et al, 2015;Carrillo-Tripp et al, 2014;Geng et al, 2014), the discovery of novel interactions in natural virus populations is likely to grow accordingly. Our results suggest that virus-virus interactions may be more common than currently recognized, and may be influential in the ecology of baculovirus and host populations.…”

Section: Discussionmentioning

confidence: 99%

Iflavirus increases its infectivity and physical stability in association with baculovirus

2016

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View full text Add to dashboard Cite

Virus transmission and the prevalence of infection depend on multiple factors, including the interaction with other viral pathogens infecting the same host. In this study, active replication of an iflavirus, Spodoptera exigua iflavirus 1 (order Picornavirales) was observed in the offspring of insects that survived following inoculation with a pathogenic baculovirus, Spodoptera exigua multiple nucleopolyhedrovirus. Tracking the origin of the iflavirus suggested the association of this virus with the occlusion bodies of the baculovirus. Here we investigated the effect of this association on the stability and infectivity of both viruses. A reduction in baculovirus pathogenicity, without affecting its infectivity and productivity, was observed when associated with the iflavirus. In contrast, viral association increased the infectivity of the iflavirus and its resistance to ultraviolet radiation and high temperature, two of the main factors affecting virus stability in the field. In addition, electron microscopy analysis revealed the presence of particles resembling iflavirus virions inside the occlusion bodies of the baculovirus, suggesting the possible co-occlusion of both viruses. Results reported here are indicative of facultative phoresis of a virus and suggest that virus-virus interactions may be more common than currently recognized, and may be influential in the ecology of baculovirus and host populations and in consequence in the use of baculoviruses as biological insecticides.

show abstract

In Search of Pathogens: Transcriptome-Based Identification of Viral Sequences from the Pine Processionary Moth (Thaumetopoea pityocampa)

Cited by 19 publications

References 69 publications

Iflavirus increases its infectivity and physical stability in association with baculovirus

Iflavirus increases its infectivity and physical stability in association with baculovirus

Draft genome and reference transcriptomic resources for the urticating pine defoliator Thaumetopoea pityocampa (Lepidoptera: Notodontidae)

Iflavirus increases its infectivity and physical stability in association with baculovirus

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