In silico transcriptome analysis of cuticle-related genes associated with delousing drug responses in the sea louse Caligus rogercresseyi

The race between host and sea lice in the Chilean salmon farming: a genomic approach

“…RNA‐Seq studies performed in C. rogercresseyi exposed to deltamethrin and azamethiphos showed changes in cuticle‐related gene expression in females and males as treatment consequences (Chávez‐Mardones et al . ). Furthermore, RNA‐Seq analysis of C. rogercresseyi after exposure to delousing drugs also showed effects in processes such as reproduction, oxidative stress and protease regulation.…”

“…), and since the expression of these genes is modulated by delousing drugs (Chávez‐Mardones et al . ), this could be associated with drug resistance patterns. SNPs were identified in practically all of the studied C. rogercresseyi biological processes suggesting associations with nervous system development (Gallardo‐Escárate et al .…”

“…Regarding moulting, a comprehensive set of genes coding for cuticle forming proteins was identified by Chávez‐Mardones et al . (), reporting higher regulation of cuticle precursors in larval stages, whereas in adults the cuticle proteins were also more expressed and with some degree of sex‐dependency.…”

“…), cuticle formation (Chávez‐Mardones et al . ), the NOTCH signalling pathway (Boltaña et al . ,), the antioxidant system (Chávez‐Mardones & Gallardo‐Escárate ; Chavez‐Mardones et al .…”

Transcriptome mining: Multigene panel to test delousing drug response in the sea louse Caligus rogercresseyi

“…β-tubulin (Table 2) was selected as the housekeeping gene (HKG) due to its stable value as inferred through the NormFinder algorithm. This HKG was used since it was previously characterized in C. rogercresseyi exposed to delousing drugs (Valenzuela-Muñoz et al, 2015;Chávez-Mardones et al, 2016). The other HKGs assayed were elongation factor alpha and β-actin, GAPDH, β-2-microglobulin and 18SrRNA.…”