In situ atomic force microscopy of partially demineralized human dentin collagen fibrils

Habelitz, Stefan; Balooch, M.; Marshall, Sally J.; Balooch, Guive; Marshall, Grayson W.

doi:10.1016/s1047-8477(02)00029-1

Cited by 250 publications

(221 citation statements)

References 42 publications

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“…Experimental work conducted by Orgel, Fratzl and others have proved that all collagen-based tissues are organized into hierarchical structures, where the lowest hierarchical level consists of triple helical collagen molecules (Fratzl, 2008;Orgel et al, 2006;Orgel et al, 2001) and the multiscale structure is defined as TC/fibrils/fibres. Also, other authors have observed a longitudinal microfibrillar structure with a width of 4 -8 nm (Habelitz et al, 2002;Baselt et al, 1993). Three-dimensional image reconstructions of 36 nm-diameter corneal collagen fibrils also showed a 4 nm repeat in a transverse section that was related to the microfibrillar structure (Holmes et al, 2001).…”

Section: Mineralized Collagen Microfibrilmentioning

confidence: 71%

Effect of material and structural factors on fracture behaviour of mineralised collagen microfibril using finite element simulation

Barkaoui¹,

Hambli²,

Tavares³

2014

Computer Methods in Biomechanics and Biomedical Engineering

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Bone is a multiscale heterogeneous material and its principal function is to support the body structure and to resist mechanical loads without fracturing. Numerical modelling of biocomposites at different length scales provides an improved understanding of the mechanical behaviour of structures such as bone, and also guides the development of multiscale mechanical models. Here, a three-dimensional nano-scale model of mineralized collagen microfibril based on the finite element method was employed to investigate the effect of material and structural factors on the mechanical equivalent of fracture properties.

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Section: Mineralized Collagen Microfibrilmentioning

confidence: 71%

Effect of material and structural factors on fracture behaviour of mineralised collagen microfibril using finite element simulation

Barkaoui¹,

Hambli²,

Tavares³

2014

Computer Methods in Biomechanics and Biomedical Engineering

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“…Scanning probe microscopes and, in particular AFM have facilitated the imaging and analysis of biological surfaces with little or no sample preparations (Habelitz et al, 2002). AFM operates in a near field with a sharp probe by scanning, enabling characterization of three-dimensional surface morphology with minimal sample preparation and high resolution.…”

Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

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“…Dentin abuts on dental enamel and acts as energy-absorbing, crackresistant structure [6]. Much effort has been devoted to understand the detailed composition and the microstructure of tubules [7][8][9][10][11][12], as well as their relationship to fracture toughness [6,13]. The interface between dentin and enamel, i.e.…”

Section: Introductionmentioning

confidence: 99%

Understanding Nano-Anatomy of Healthy and Carious Human Teeth: a Prerequisite for Nanodentistry

et al. 2012

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The anatomy of human teeth reflects its usage. Spatially resolved X-ray scattering permits quantitative studies of the characteristic arrangement of the anisotropic calcium phosphate crystallites and the collagen fibers within the hard tissues of the crown. The present study summarizes the distinctive nanometer-sized anatomical features of the tooth hard tissues including their interface taking advantage of spatially resolved synchrotron radiation-based small-angle X-ray scattering. The comparison of slices from eight teeth indicates a long-range organization of tooth nanostructures.

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In situ atomic force microscopy of partially demineralized human dentin collagen fibrils

Cited by 250 publications

References 42 publications

Effect of material and structural factors on fracture behaviour of mineralised collagen microfibril using finite element simulation

Effect of material and structural factors on fracture behaviour of mineralised collagen microfibril using finite element simulation

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Understanding Nano-Anatomy of Healthy and Carious Human Teeth: a Prerequisite for Nanodentistry

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