In situ chemically specific mapping of agrochemical seed coatings using stimulated Raman scattering microscopy

Wang, Chun-Chin; Moorhouse, S; Stain, Chris; Seymour, Mark; Green, Ellen; Penfield, Steven; Moger, Julian

doi:10.1002/jbio.201800108

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…The drug penetrated through the lipid-rich structures, consistent with its lipophilic properties (119). SRS imaging has also revealed distribution of the fungicide fludioxonil in the seed coat based on its intrinsic CࣕN bond (114).…”

Section: Imaging Small-molecule Drugs and Other Functional Moleculesmentioning

confidence: 63%

Raman Imaging of Small Biomolecules

Shen

Min

2019

Annu. Rev. Biophys.

115

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Imaging techniques greatly facilitate the comprehensive knowledge of biological systems. Although imaging methodology for biomacromolecules such as protein and nucleic acids has been long established, microscopic techniques and contrast mechanisms are relatively limited for small biomolecules, which are equally important participants in biological processes. Recent developments in Raman imaging, including both microscopy and tailored vibrational tags, have created exciting opportunities for noninvasive imaging of small biomolecules in living cells, tissues, and organisms. Here, we summarize the principle and workflow of small-biomolecule imaging by Raman microscopy. Then, we review recent efforts in imaging, for example, lipids, metabolites, and drugs. The unique advantage of Raman imaging has been manifested in a variety of applications that have provided novel biological insights.

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Section: Imaging Small-molecule Drugs and Other Functional Moleculesmentioning

confidence: 63%

Raman Imaging of Small Biomolecules

Shen

Min

2019

Annu. Rev. Biophys.

115

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“…A set‐up with more laser power available on the sample, combined with a more efficient collection path, would make imaging at long wavelengths a viable option for improving depth penetration of SRS imaging techniques in practical applications. The latter would include nonbiological samples and biological samples with a low water content, such as agrochemical seed coatings …”

Section: Discussionmentioning

confidence: 99%

Stimulated Raman scattering microscopy with long wavelengths for improved imaging depth

Moester

Zada

Fokker

et al. 2018

J Raman Spectroscopy

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Stimulated Raman scattering (SRS) imaging is a fast, label‐free, and sensitive technique to map the distribution of a vibrational species in a microscopy setting. It has great potential for applications in many fields, such as lipid imaging in biomedicine. However, depth penetration of the light into the sample is an issue with any light‐based technique, especially with multiphoton techniques such as SRS. Using longer wavelengths allows deeper penetration into densely scattering materials, but applying wavelengths above 1,500 nm is challenging technically. We have built a flexible SRS microscope system capable of imaging with a combination of 1,064 nm and wavelengths over 1,500 nm, using the idler output of an optical parametric oscillator (OPO). For comparison, the same system was also operated in the more common configuration, using 1,064 nm in combination with the OPO signal output around 800 nm. With the long‐wavelength settings, we show improved depth penetration in polyethylene plastic material and in a silicone phantom with embedded polymer microbeads, and we report images of lipid structure in biological tissue. These results demonstrate the technical feasibility of using these long wavelengths for SRS imaging. Disadvantages such as poorer spatial resolution and lower signal strength are also discussed. The application of this new approach to SRS microscopy can allow greater insight into deep‐lying structures in a non‐invasive way.

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“…[ 119 ] Many agrochemicals do not contain Raman vibrations in the silent region; hence, deuterium labeling was introduced to assist chemically specific imaging of these compounds. [ 70,120 ] ABL1 tyrosine‐kinase inhibitors (TKIs) are well‐known targeted cancer medicines. The mechanism by which their efficacy is increased via lysosome‐mediated drug‐drug interactions was investigated by using hyperspectral SRS to monitor TKI drugs (imatinib and nilotinib) in living cells.…”

Section: Applicationsmentioning

confidence: 99%

Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences

Shen

et al. 2020

Advanced Biology

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elastically scattered photons) featuring time-consuming spectral integration and hence a poor acquisition speed (several seconds per line or minutes per frame). This weakness constitutes a large obstacle for real-time monitoring of dynamic events in organisms. Recently, several variations of Raman spectroscopy have been developed to enhance the sensitivity of Raman spectroscopy and dynamic processes of biological samples, including coherent Raman scattering (CRS) spectroscopy and surfaceenhanced Raman spectroscopy (SERS). SERS involves a plasmonic effect where molecules absorbed on a rough metal surface can result in high Raman scattering intensities by increasing the incident electric field. [5] For SERS technology, a SERS substrate must be introduced to enhance the detection sensitivity, and the properties of the substrate (the composition, size, shape and aggregation degree of nanoparticles) affect the shape of the SERS spectra. In addition, only gold, silver, copper, and a few rare alkali metals (such as lithium and sodium) have strong SERS effects, and it is necessary to effectively solve the biocompatibility and safety issues in the preparation of nanomaterials. With the development of laser and nonlinear optics, CRS microscopy has also been demonstrated to break the speed limit for vibrational imaging. [6] CRS has two major forms: coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS); [7] both techniques can dramatically increase the Raman signal by a few orders of magnitude and hence are able to achieve video-rate molecular imaging in vivo. [8,9] CARS and SRS processes often exist in a sample concurrently because of the same excitation conditions (spatiotemporal overlap of the pump and Stokes beam) with a frequency difference (Raman shift) matching the molecular vibrational frequency. However, CARS is an optical parametric process accompanied by a nonresonant part originating from fourwave mixing processes. This intrinsic nonresonant background results in a deviation or even distortion of the Raman spectrum. SRS is a direct process of photon-vibration energy transfer: in brief, the interaction between the electron cloud of a sample and the photons of two lasers creates an induced dipole moment within the molecule based on its polarizability, resulting in a pump energy loss (stimulated Raman loss, SRL) and a Stokes energy increase (stimulated Raman gain, SRG). The Raman signal can, therefore, be deduced from SRL or SRG, resulting in a high-fidelity Raman spectrum free from the nonresonant background. Moreover, the SRS signal exhibits Stimulated Raman scattering (SRS) microscopy is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds. Featuring high speed, high resolution, high sensitivity, high accuracy, and 3D sectioning, SRS microscopy has made tremendous progress toward biochemical information acquisition, cellular function investigation, and label-free medical diagnosis in the biosciences. In this review, the principle of ...

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In situ chemically specific mapping of agrochemical seed coatings using stimulated Raman scattering microscopy

Cited by 10 publications

References 44 publications

Raman Imaging of Small Biomolecules

Raman Imaging of Small Biomolecules

Stimulated Raman scattering microscopy with long wavelengths for improved imaging depth

Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences

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