In situ digestion of detergent fiber nitrogen in alfalfa stems

Sanderson, Matt A.; Wedin, W. F.

doi:10.1016/0377-8401(90)90047-c

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…Arroyo & González (2013 b ) also showed a high ED of ADIN close to that of NDIN in Italian ryegrass hay. High apparent in situ degradations have been shown previously in lucerne stems (Sanderson & Wedin 1990). Also, apparent in situ results for lucerne of Aufrère et al (1994) showed a large degradation of ADIN as well as in one of its main constituents, extensin, a glycoprotein of the primary cell wall.…”

Section: Discussionsupporting

confidence: 60%

Effects of correcting for microbial contamination and the use of sodium sulphite in neutral detergent fibre analyses on the ruminal fibre degradability of several feeds

et al. 2014

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Sodium sulphite is used in an optional way to remove insoluble proteins from neutral detergent fibre (NDF) residue. To determine whether the recovery of both NDF and insoluble nitrogen (N) in NDF solution (NDIN) are altered by its use, both parameters were measured in a set of 12 feeds, including cereal grains: maize (MG), rye (RG) and wheat (WG); cereal co-products: maize gluten feed (MGF), distilled dried grains from barley (DDGB) and wheat (DDGW) and wheat bran (WB); protein concentrates: rapeseed meal (RSM) and expeller palm kernel (EPK); dehydrated sugar beet pulp (DBP) and oat (OH) and ryegrass (RGH) hays. Associated effects on the in situ effective degradability (ED) of both NDF and NDIN were also studied in DDGW, WB, RSM, EPK, DBP, OH and RGH. Also, ED of acid detergent fibre (ADF) and its N (ADIN) were studied in hays. Errors due to microbial contamination in the rumen on the ED of NDF, ADF, NDIN and ADIN were also established in these last seven samples using 15 N infusion methods. Three rumen and duodenum cannulated wethers were used in the study. The sulphite use in NDF solution led to reductions (DDGB, DDGW, RSM and OH) and increases (RG, WG, WB and DBP) of the NDIN proportion, as well as the contribution of crude protein to NDF. These variations were associated with irregular effects on NDF residues and on ED of both NDIN and NDF. As a consequence, sulphite use does not assure the reduction of the insoluble protein contamination and it may even increase it. This methodology may also alter the degradability estimates of NDIN or NDF. Mean ruminal microbial contamination in NDF was 7·0, 10·8, 13·3, 5·4, 12·0, 35·3 and 20·0 g/kg in WB, DDGW, RSM, EPK, DBP, OH and RGH, respectively. The associated contents of microbial N in NDIN were: 59·3, 29·9, 26·2, 19·8, 37·3, 441 and 150 g/kg, respectively. Microbial contamination in ADF and ADIN (g/kg) was 3·6 and 94·5 in OH and 1·7 and 41·2 in RGH. Not correcting this contamination led to consistent undervaluations of ED of NDIN and NDF in all tested feeds, although errors only reached significance for NDIN in hays and DBP. Microbial-corrected ED of NDIN was 0·685, 0·826, 0·481, 0·389, 0·166, 0·718 and 0·425 in WB, DDGW, RSM, EPK, DBP, OH and RGH, respectively, whereas values for ADIN were 0·504 (OH) and 0·469 (RGH).

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Section: Discussionsupporting

confidence: 60%

Effects of correcting for microbial contamination and the use of sodium sulphite in neutral detergent fibre analyses on the ruminal fibre degradability of several feeds

et al. 2014

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“…The proportion of N attached to the ADFN, which is undegradable and undigestible, was low, as expected for these kinds of forages (Fall-Toure and Michalet-Doreau 1995). On the other hand, ADFN increased with maturity, as reported by Sanderson and Wedin (1990) for high-quality forages.…”

Section: Discussionmentioning

confidence: 51%

Oat and Oat-Vetch as Rainfed Fodder-Cover Crops in Semiarid Environments: Effects of Fertilization and Harvest Time on Forage Yield and Quality

Ramos¹,

Altieri²,

Garcia³

et al. 2011

Journal of Sustainable Agriculture

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We evaluate the forage yield and quality, species composition, and carrying capacity of oats and oat-vetch as fodder-cover crops, grown in almond orchards, under mineral or organic fertilizer and three harvesting regimes ("grazing," "hay," and "grain plus straw"). Also, we show preliminary results of almond yield. Differences in yield due to mineral and organic fertilization were only 9%. The carrying capacity was similar for all treatments. The almond yield was greater when the cover crop was removed early as "grazing." Therefore, oats and oat-vetch could be used as cover crops in almond orchards if removed early by livestock grazing.

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“…The N content of grass and the disappearance of N from nylon bags in the rumen, expressed as the EPD value, decreased with increasing maturity, as found elsewhere (Lindberg, 1988;Sanderson & Wedin, 1990;Van Vuuren et al, 1991;Messman et al, 1992). However, the fit of the degradation data on the latest-cut grass into an equation based on a first-order reaction may be questioned, as indicated by the long negative lag time, which differed markedly from that of other grasses ( Table 2).…”

Section: Ruminal Disappearance Of Nmentioning

confidence: 77%

“…Here, we also calculated the curve from IDN estimated after grass N had been pre-incubated in the rumen for 96 h before passage through the intestine in the mobile bags. The difference between the contents of NDFN and ADFN in samples, expressed as available fibrebound N (AFN) (Sanderson & Wedin, 1990), was also calculated. The disappearance of AFN from nylon bags in the rumen and from mobile bags within the intestine was subsequently calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Assuming that soluble N in grass degrades completely in the rumen, the content of NDFN in rumenundegradable feed residue may represent N that is of dietary origin only (Lindberg, 1988;Sanderson & Wedin 1990). As the microbes attached to feed residues in the rumen should be soluble in neutral detergent, the NDFN in rumen-undegradable feed residue should be free from non-feed N. Accordingly, the same assumption can be made regarding microbes becoming attached to feed residues in mobile bags in the lower tract.…”

Section: Intestinal Disappearance Of Fibre-bound Nmentioning

confidence: 99%

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Effects of Stage of Growth and Duration of Rumen Incubation Time on Intestinal Digestibility of Rumen-Undegradable Nitrogen of Grass by Mobile-Bag Method in Cows

Vanhatalo

Dakowski

Huhtanen

1996

Acta Agriculturae Scandinavica, Section A — Animal Science

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Effects of stage of growth and duration of rumen incubation time on intestinal digestibility of rumen-undegradable nitrogen of grass by mobile-bag method in cows.The ruminal and intestinal degradability of the nitrogen constituents of timothy grass cut at five different stages of growth was studied in three non-lactating cows. The grass samples were ihcubated in nylon bags in the rumen for 0, 6, 12, 24, 48, 72 and 96 h. The subsequent digestion of rumen-undegradable N in the intestine was measured by the mobile-bag method. The N content of grass declined, and the fibre content and the proportion of N bound in acid detergent fibre increased with increasing maturity. The values for rumen degradability of grass N corrected for microbial contamination were generally high and they decreased with increasing maturity. The intestinal N digestibility of rumen-undegradable N of grass ranged from 49% to 78%, being affected by growth stage and the duration of preceding rumen incubation time. These values may, however, be confounded by nonfeed N. Pre-incubation of forage samples in the rumen indicated that at least 48 h of rumen incubation is needed for predicting intestinal N digestibility from the indigestible N fraction determined with the mobile-bag method.

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In situ digestion of detergent fiber nitrogen in alfalfa stems

Cited by 7 publications

References 16 publications

Effects of correcting for microbial contamination and the use of sodium sulphite in neutral detergent fibre analyses on the ruminal fibre degradability of several feeds

Effects of correcting for microbial contamination and the use of sodium sulphite in neutral detergent fibre analyses on the ruminal fibre degradability of several feeds

Oat and Oat-Vetch as Rainfed Fodder-Cover Crops in Semiarid Environments: Effects of Fertilization and Harvest Time on Forage Yield and Quality

Effects of Stage of Growth and Duration of Rumen Incubation Time on Intestinal Digestibility of Rumen-Undegradable Nitrogen of Grass by Mobile-Bag Method in Cows

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