In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Kim, Hyeong Rae; An, Sanggwon; Hwang, Jungho; Park, Jae Hong; Byeon, Jeong Hoon

doi:10.1016/j.jhazmat.2019.02.088

Cited by 13 publications

(5 citation statements)

References 37 publications

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“…The collection efficiency of the SKC BioSampler for 100 nm sized particles, which is the known size of SARS-CoV-2, has been reported as 30–40% [ 14 ]. The Swab sampler, a useful air sampler for capturing airborne viruses, utilizes a cotton swab that acts as a filter for capturing airborne particles with 99% efficacy for airborne viruses, as per a previous report [ 15 ]. Air sampling was carried out at 1.2 m above floor level and at a distance of 1.0 m from the patient in the negative pressure rooms.…”

Section: Methodsmentioning

confidence: 99%

Environmental contamination in the isolation rooms of COVID-19 patients with severe pneumonia requiring mechanical ventilation or high-flow oxygen therapy

Ahn

Sohn

et al. 2020

Journal of Hospital Infection

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Summary Background Identifying the extent of environmental contamination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for infection control and prevention. The extent of environmental contamination has not been fully investigated in the context of severe coronavirus disease (COVID-19) patients. Aim To investigate environmental SARS-CoV-2 contamination in the isolation rooms of severe COVID-19 patients requiring mechanical ventilation or high-flow oxygen therapy. Methods We collected environmental swab samples and air samples from the isolation rooms of three COVID-19 patients with severe pneumonia. Patient 1 and Patient 2 received mechanical ventilation with a closed suction system, while Patient 3 received high-flow oxygen therapy and noninvasive ventilation. Real-time reverse transcription polymerase chain reaction (rRT-PCR) was used to detect SARS-CoV-2; viral cultures were performed for samples not negative on rRT-PCR. Findings Of the 48 swab samples collected in the rooms of Patient 1 and Patient 2, only samples from the outside surfaces of the endotracheal tubes tested positive for SARS-CoV-2 by rRT-PCR. However, in Patient 3’s room, 13 of the 28 environmental samples (fomites, fixed structures, and ventilation exit on the ceiling) showed positive results. Air samples were negative for SARS-CoV-2. Viable viruses were identified on the surface of the endotracheal tube of Patient 1 and seven sites in Patient 3’s room. Conclusion Environmental contamination of SARS-CoV-2 can be a route of viral transmission. However, it might be minimized when patients receive mechanical ventilation with a closed suction system. These findings can provide evidence for guidelines for the safe use of personal protective equipment.

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Section: Methodsmentioning

confidence: 99%

Environmental contamination in the isolation rooms of COVID-19 patients with severe pneumonia requiring mechanical ventilation or high-flow oxygen therapy

Ahn

Sohn

et al. 2020

Journal of Hospital Infection

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“…(a) swab (from ref. [31] ); (b) automated bioaerosol-monitoring system (from ref. [38] ); (c) electrostatic sampler with swab (from ref.…”

Section: Adenosine Triphosphate Bioluminescence Assaymentioning

confidence: 99%

“…As shown in Fig. 2 a, under the control of an Arduino-Bluetooth smartphone controller, near real-time (< 3 min) monitoring of both aerosolized Staphylococcus aureus and field bioaerosols was achieved by efficiently extracting ATP from bioaerosols captured in microfiber swabs and amplifying the bioluminescence signal (> 10 4 relative luminescent units (RLU)/m 3 air) [31] . Yoon et al .…”

Section: Adenosine Triphosphate Bioluminescence Assaymentioning

confidence: 99%

Recent progress in online detection methods of bioaerosols

Liang

Chen

et al. 2024

Fundamental Research

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“…Adenosine triphosphate (ATP), as an energy substance, is commonly found in live cells [ 131 ]. The ATP-based bioluminescence assay is based on the measurement of the light resulted from the reaction between luciferin and luciferase.…”

Section: Pathogen Detectionmentioning

confidence: 99%

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Lei

et al. 2021

Micromachines

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As an important route for disease transmission, bioaerosols have received increasing attention. In the past decades, many efforts were made to facilitate the development of bioaerosol monitoring; however, there are still some important challenges in bioaerosol collection and detection. Thus, recent advances in bioaerosol collection (such as sedimentation, filtration, centrifugation, impaction, impingement, and microfluidics) and detection methods (such as culture, molecular biological assay, and immunological assay) were summarized in this review. Besides, the important challenges and perspectives for bioaerosol biosensing were also discussed.

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In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Cited by 13 publications

References 37 publications

Environmental contamination in the isolation rooms of COVID-19 patients with severe pneumonia requiring mechanical ventilation or high-flow oxygen therapy

Environmental contamination in the isolation rooms of COVID-19 patients with severe pneumonia requiring mechanical ventilation or high-flow oxygen therapy

Recent progress in online detection methods of bioaerosols

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

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