In Situ Precision Cell Electrospinning as an Efficient Stem Cell Delivery Approach for Cutaneous Wound Healing

Abstract

Mesenchymal stem cell (MSC) therapies have been brought forward as a promising treatment modality for cutaneous wound healing. However, current approaches for stem cell delivery have many drawbacks, such as lack of targetability and cell loss, leading to poor efficacy of stem cell therapy. To overcome these problems, in the present study, an in situ cell electrospinning system is developed as an attractive approach for stem cell delivery. MSCs have a high cell viability of over 90% even with a high applied voltage of 15 kV post‐cell electrospinning process. In addition, cell electrospinning does not show any negative effect on the surface marker expression and differentiation capacity of MSCs. In vivo studies demonstrate that in situ cell electrospinning treatment can promote cutaneous wound healing through direct deposition of bioactive fish gelatin fibers and MSCs onto wound sites, leading to a synergic therapeutic effect. The approach enhances extracellular matrix remodeling by increasing collagen deposition, promotes angiogenesis by increasing the expression of vascular endothelial growth factor (VEGF) and forming small blood vessels, and dramatically reduces the expression of interleukin‐6 (IL‐6) during wound healing. The use of in situ cell electrospinning system potentially provides a rapid, no touch, personalized treatment for cutaneous wound healing.

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

“…Furthermore, it also increased the expression of vascular endothelial growth factor (VEGF) and the formation of small blood vessels to promote angiogenesis but significantly reduced the expression of interleukin-6 (IL-6), and ultimately promoted skin wound healing [107]. When comparing poly (ε-caprolactone) (PCL) and poly (ε-caprolactone)/type I collagen (PCol) in the ability to promote biological signaling, wound coverage, and tissue repair processes, it was found that PCol/human Wharton's jelly mesenchymal stromal cells hWJ-MSCs had a better effect on skin tissue repair [108].…”

Dual-Drug-Loaded Core–Shell Electrospun Nanofiber Dressing for Deep Burns

“…As the largest organ on the surface of the human body, the skin has the basic ability of thermoregulation, immunity, and resistance to external bacteria and viruses. , Deep burns resulting from high temperatures or electric shocks can lead to damage to both the epidermis and a portion of the dermis in the skin. It can cause intense pain and pose a significant threat to life if not promptly treated.…”

The synergetic effect of alginate-derived hydrogels and metal–phenolic nanospheres for chronic wound therapy

“…Wound dressings represent a current approach in the treatment of diabetic ulcers, encompassing film dressings, 21,22 electrospun nanofibers, 23,24 hydrophilic colloids and hydrogels. 25–27 Among them, hydrogels show tremendous potential in wound healing therapy due to its unique extracellular matrix-like (ECM) three-dimensional (3D) structure, long-lasting moisturizing properties, and drug encapsulation capability.…”