In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells

Gholipour, Yousef; Erra‐Balsells, Rosa; Nonami, Hiroshi

doi:10.5702/massspectrometry.a0003

Cited by 16 publications

(21 citation statements)

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“…By recording depth of penetration and with anatomy information in hand, the cell layer in which capillary is located can be determined with a position indicator of a piezomotor manipulator system (Gholipour et al, 2012). In addition, it is uniquely possible to access and sample a deeper cell with minimum contaminations of shallower cell solution (Gholipour et al, 2012).…”

Section: The Techniquementioning

confidence: 99%

“…Unlike other cell sampling methods based on the penetration of simple glass capillary tip (Tejedor et al, 2009) or of a metal needle (as in probeelectrospray, Yu et al, 2009), use of a cell PP provided fully manageable probing and picoliter sampling that included confirmation of capillary localization, the absence of leakage (hydraulic continuity test), and cell sample volume measurement (Gholipour et al, 2012). The cell type and location can be distinguished by turgor pressure probing (Gholipour et al, 2012). By recording depth of penetration and with anatomy information in hand, the cell layer in which capillary is located can be determined with a position indicator of a piezomotor manipulator system (Gholipour et al, 2012).…”

Section: The Techniquementioning

confidence: 99%

“…A part of cell solution sample is loaded onto a cryoosmometer plate to measure osmotic potential (Shackel, 1987;Nonami and Schulze, 1989) as shown in a photo in Gholipour et al (2012). Water potential of the cell is then obtained by summing up value of turgor and that of osmotic potential.…”

Section: Plant Materialsmentioning

confidence: 99%

“…By summing the turgor pressure ( P) and osmotic potential ( S) values, water potential ( W) of the cell is calculated ( W P S). PP can be also used for cell volume measurement after turgor measurement (Malone and Tomos, 1990;Gholipour et al, 2012). Capillary movement and penetration depth were monitored and measured carefully by a piezo manipulator (PM101, Märzhäuser Wetzlar, Germany) and corresponding cells were identified under microscope for volume measurement (Gholipour et al, 2012).…”

Section: Plant Materialsmentioning

confidence: 99%

See 3 more Smart Citations

Blossom End Rot Tomato Fruit Diagnosis for <i>In Situ</i> Cell Analyses with Real Time Pico-Pressure Probe Ionization Mass Spectrometry

Gholipour

Balsells

Nonami

2017

Environmental Control in Biology

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By combining a cell pressure probe and an Orbitrap mass spectrometer, quantitative snapshot profiles of metabolites of in situ plant single cells during development of blossom end rot in tomato (Solanum lycopersicum L.) fruit were analyzed while tomato plants were grown hydroponically in a greenhouse. By using the pressure probe, cell turgor, cell volume, cell wall elastic modulus, and hydraulic conductivity of plasma membrane were measured, followed by managed cytoplasm sampling, and osmotic and water potentials determination. It was found that the cell bursting results from alterations in water relations and cell wall properties accompanied with changes in metabolites of cells located at blossom end rot area in tomato fruit. From the water relations point of view, the loss of the elasticity and cell wall weakening, with decreased water potential and excess turgor resulted in mechanical rupture of membrane and cell wall. Abscisic acid was detected in damaged cells as a possible evidence of triggered precocious maturity. Simultaneously, a sharp rise in the concentration of phenols (coumarinate-glucoside and chlorogenic acid) and salicylic acid and decline in ascorbic acid reflected the activation of cell death process that would facilitate the deterioration of cell wall and plasma membrane.

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Section: The Techniquementioning

confidence: 99%

Section: The Techniquementioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

See 2 more Smart Citations

Blossom End Rot Tomato Fruit Diagnosis for <i>In Situ</i> Cell Analyses with Real Time Pico-Pressure Probe Ionization Mass Spectrometry

Gholipour

Balsells

Nonami

2017

Environmental Control in Biology

Self Cite

View full text Add to dashboard Cite

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“…Before the end of desorption, the surface temperature decreased and thermal desorption at low vapor pressure was the dominant process. Under these conditions, the velocities become small and the In situ pressure probe sampling and UV-MALDI MS for profiling metabolites in living single cells Plant cells 37 (Gholipour et al, 2012) Identification of pathogens Several techniques. MALDI detection of lipid A etc.…”

Section: Theorymentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Harvey

2015

Mass Spectrometry Reviews

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This review is the seventh update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2012. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, and fragmentation are covered in the first part of the review and applications to various structural types constitute the remainder. The main groups of compound are oligo- and poly-saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2015 Wiley Periodicals, Inc. Mass Spec Rev 36:255-422, 2017.

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Application of porous metal enrichment probe sampling to single cell analysis using matrix‐assisted laser desorption ionization time of flight mass spectrometry (MALDI‐TOF‐MS)

Tang

Cui

et al. 2015

J. Mass Spectrom.

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There is an increasing need for analyzing metabolism in a single cell, which is important to understand the nature of cellular heterogeneity, disease, growth and specialization, etc. However, single cell analysis is often challenging for the traces of samples. In the present study, porous metal enrichment probe sampling combined with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) has been applied for in situ analysis of live onion epidemic cell. Porous probe, treated by corroding copper wire with HCl, was directly inserted into a single cell to get cell solution. A self-made linear actuator was enough to control the penetration of probe into the target cell accurately. Then samples on the tip of probe were eluted and detected by a commercial MALDI-TOF-MS directly. The formation of porous microstructure on the probe surface increased the adsorptive capacity of cell solution. The sensitivity of porous probe sampling was 6 times higher than uncorroded probes generally. This method provides a sensitive and convenient way for the sampling and detection of single cell solution. Copyright © 2015 John Wiley & Sons, Ltd.

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In Situ Pressure Probe Sampling and UV-MALDI MS for Profiling Metabolites in Living Single Cells

Cited by 16 publications

References 50 publications

Blossom End Rot Tomato Fruit Diagnosis for <i>In Situ</i> Cell Analyses with Real Time Pico-Pressure Probe Ionization Mass Spectrometry

Blossom End Rot Tomato Fruit Diagnosis for <i>In Situ</i> Cell Analyses with Real Time Pico-Pressure Probe Ionization Mass Spectrometry

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2011–2012

Application of porous metal enrichment probe sampling to single cell analysis using matrix‐assisted laser desorption ionization time of flight mass spectrometry (MALDI‐TOF‐MS)

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