In situ reverse-transcription loop-mediated isothermal amplification (in situ RT-LAMP) for detection of Japanese encephalitis viral RNA in host cells

Liu, Yi; Chuang, Ching-Kai; Chen, Wei-June

doi:10.1016/j.jcv.2009.06.010

Cited by 20 publications

(16 citation statements)

References 39 publications

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“…The cultivation of BHK-21 cells and amplification of JE virus stock are described in previous studies [ 9 , 10 ]. The JE virus used in this study was the T1P1 strain isolated from field-caught Armigeres subalbatus [ 11 ] (provided by Wei-June Chen, Chang Gung University, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles

Wang

Chen

et al. 2016

Viruses

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Evidence has emerged that RNA viruses utilize the host secretory pathway for processing and trafficking mature viral particles and for exiting the infected cells. Upon completing the complex assembly process, the viral particles take advantage of the cellular secretory trafficking machinery for their intracellular trafficking toward the Golgi organelle and budding or export of virions. In this study, we showed that Japanese encephalitis virus (JEV)-induced extracellular GRP78 contains no KDEL motif using an anti-KDEL-specific antibody. Overexpression of the KDEL-truncated GRP78 in the GPR78 knocked down cells significantly reduced JEV infectivity, suggesting that the KDEL motif is required for GRP78 function in the release of JE viral particles. In addition, we demonstrated the KDELR protein, an ER-Golgi retrieval system component, is associated with viral envelope proteins and is engaged in the subcellular localization of viral particles in Golgi. More importantly, accumulation of intracellular virions was observed in the KDELR knocked down cells, indicating that the KDELR protein mediated the intracellular trafficking of JE viral particles. Altogether, we demonstrated that intracellular trafficking of JE assembled viral particles was mediated by the host ER-Golgi retrieval system prior to exit by the secretory pathway.

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Section: Methodsmentioning

confidence: 99%

A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles

Wang

Chen

et al. 2016

Viruses

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“…Recently, derivative LAMP assays, such as reversetranscription LAMP assay [12], multiplex LAMP assay [40], in situ LAMP assay [38,116], and real-time reversetranscription LAMP assay [55], have been developed and employed for the detection of various foodborne pathogens, such as Bacillus anthracis [79], Vibrio parahaemolyticus [69,113,120], Staphylococcus aureus [114], Salmonella [13,116,119], Pseudomonas aeruginosa [121], Escherichia coli O157 [71,118], and Listeria monocytogenes [105].…”

Section: Isothermal Amplificationmentioning

confidence: 99%

Advances in Rapid Detection Methods for Foodborne Pathogens

Zhao¹,

Lin²,

Wang³

et al. 2014

Journal of Microbiology and Biotechnology

597

377

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Food safety is increasingly becoming an important public health issue, as foodborne diseases present a widespread and growing public health problem in both developed and developing countries. The rapid and precise monitoring and detection of foodborne pathogens are some of the most effective ways to control and prevent human foodborne infections. Traditional microbiological detection and identification methods for foodborne pathogens are well known to be time consuming and laborious as they are increasingly being perceived as insufficient to meet the demands of rapid food testing. Recently, various kinds of rapid detection, identification, and monitoring methods have been developed for foodborne pathogens, including nucleic-acid-based methods, immunological methods, and biosensor-based methods, etc. This article reviews the principles, characteristics, and applications of recent rapid detection methods for foodborne pathogens.

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“…JEV RT-LAMP assay has improved sensitivity compare to routine RT-PCR with equal sensitivity to qRT -PCR assay with the detection limits ranging between 0.1 and 10 plaque forming units (PFUs) of virus (Toriniwa & Komiya, 2006). Recently, digoxigenin was combined with the deoxycytidine triphosphate reaction mixture as a new detection method for detecting JEV in blood samples with low viral genome copies (Liu et al, 2009). RT-LAMP coupled with a lateral flow dipstick is used to detect multiple strains of JEV which eliminate the need for potentially unstable fluorescent dyes (Deng et al, 2015).…”

Section: Molecular Diagnosismentioning

confidence: 99%

Japanese Encephalitis, Recent Perspectives on Virus Genome, Transmission, Epidemiology, Diagnosis and Prophylactic Interventions

Karthikeyan¹,

Shanmuganathan²,

Pavulraj³

et al. 2017

JEBAS

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In situ reverse-transcription loop-mediated isothermal amplification (in situ RT-LAMP) for detection of Japanese encephalitis viral RNA in host cells

Abstract: The newly designed method can detect viral RNAs in peripheral blood mononuclear cells (PBMCs) in a short time with high sensitivity and efficiency.

Cited by 20 publications

References 39 publications

A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles

A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles

Advances in Rapid Detection Methods for Foodborne Pathogens

Japanese Encephalitis, Recent Perspectives on Virus Genome, Transmission, Epidemiology, Diagnosis and Prophylactic Interventions

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