In situ seed baiting techniques in Dendrobium officinale Kimuraet Migo and Dendrobium nobile Lindl. : the endangered Chinese endemic Dendrobium (Orchidaceae)

Wang, Hui; Fang, Hao; Wang, Yunqiang; Li-sheng, Duan; Guo, Shun‐Xing

doi:10.1007/s11274-011-0667-9

Cited by 47 publications

(35 citation statements)

References 16 publications

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“…Similarly, fungi from the protocorm phase of D. chrysanthum were mainly anamorphic Sebacinaceae, which differ from those isolated from the protocorms of D. nobile. Moreover, preliminary symbiosis germination experiments have demonstrated that two strains (3597 and 3601), originating from the protocorms of D. chrysanthum that were found in the seeds packets, can promote seed germination and seedling development in vitro of D. candidum and D. nobile (Wang et al 2011), while isolate 3564 has not had any conspicuous effects on the germination of D. nobile. This implies that D. nobile may not be a suitable mycorrhizal host for fungi from D. nobile protocorms or that the seed germination of Dendrobium species requires the presence of specific fungal species (Sebacinales but not Tulasnella spp.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

2011

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The seed germination of orchids under natural conditions requires association with mycorrhizal fungi. Dendrobium nobile and Dendrobium chrysanthum are threatened orchid species in China where they are considered medicinal plants. For conservation and application of Dendrobium using symbiosis technology, we isolated culturable endophytic and mycorrhizal fungi colonized in the protocorms and adult roots of two species plants and identified them by morphological and molecular analyses (5.8S and nrLSU). Of the 127 endophytic fungi isolated, 11 Rhizoctonia-like strains were identified as Tulasnellales (three strains from protocorms of D. nobile), Sebacinales (three strains from roots of D. nobile and two strains from protocorms of D. chrysanthum) and Cantharellales (three strains from roots of D. nobile), respectively. In addition, species of Xylaria, Fusarium, Trichoderma, Colletotrichum, Pestalotiopsis, and Phomopsis were the predominant non-mycorrhizal fungi isolated, and their probable ecological roles in the Dendrobium plants are discussed. These fungal resources will be of great importance for the large-scale cultivation of Dendrobium plants using symbiotic germination technology and for the screening of bioactive metabolites from them in the future.

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Section: Discussionmentioning

confidence: 99%

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

2011

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“…One group of seeds was cultured with Sebacina sp., which was isolated from D . officinale symbiotic seeds [8] (Genbank number EU910926 and public culture collection accession number CGMCC No. 3398) on oatmeal agar (OMA) medium [21] was germinated to the third stage, which is characterized by the appearance of protomeristem [22].…”

Section: Methodsmentioning

confidence: 99%

“…Several species of fungi that engage in symbiosis and provide nutrients, such as nitrogen (N) and carbon (C), during orchid seed germination have been isolated and characterized. Most of these species have been classified as orchid-associated Rhizoctonia-type fungi [6,7], in which Tulasnellaceae, Sebacinaceae and Ceratobasidiaceae have been reported to support seed germination and protocorm development [6,8,9]. …”

Section: Introductionmentioning

confidence: 99%

“…officinale has been used in traditional Chinese medicine as a Yin tonic to nourish the stomach, relieve throat inflammation and fatigue, promote the secretion of body fluids, reduce peripheral vascular obstruction, prevent the development of cataracts and enhance the immune system. However, limited natural resources and high demand threaten the survival of the species, which has been listed as an endangered species and catalogued in the Chinese Plant Red Book since 1987 [8]. The low germination rate under natural conditions is the main factor that limits D .…”

Section: Introductionmentioning

confidence: 99%

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ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale

Zhao

Zhang

et al. 2013

PLoS ONE

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Dendrobium officinale (Orchidaceae) is one of the world’s most endangered plants with great medicinal value. In nature, D . officinale seeds must establish symbiotic relationships with fungi to germinate. However, the molecular events involved in the interaction between fungus and plant during this process are poorly understood. To isolate the genes involved in symbiotic germination, a suppression subtractive hybridization (SSH) cDNA library of symbiotically germinated D . officinale seeds was constructed. From this library, 1437 expressed sequence tags (ESTs) were clustered to 1074 Unigenes (including 902 singletons and 172 contigs), which were searched against the NCBI non-redundant (NR) protein database (E-value cutoff, e-5). Based on sequence similarity with known proteins, 579 differentially expressed genes in D . officinale were identified and classified into different functional categories by Gene Ontology (GO), Clusters of orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expression levels of 15 selected genes emblematic of symbiotic germination were confirmed via real-time quantitative PCR. These genes were classified into various categories, including defense and stress response, metabolism, transcriptional regulation, transport process and signal transduction pathways. All transcripts were upregulated in the symbiotically germinated seeds (SGS). The functions of these genes in symbiotic germination were predicted. Furthermore, two fungus-induced calcium-dependent protein kinases (CDPKs), which were upregulated 6.76- and 26.69-fold in SGS compared with un-germinated seeds (UGS), were cloned from D . officinale and characterized for the first time. This study provides the first global overview of genes putatively involved in D . officinale symbiotic seed germination and provides a foundation for further functional research regarding symbiotic relationships in orchids.

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“…The PLBs at the stage 3 (Stewart et al 2003) were maintained in hormone-free 1/2 Murashige-Skoog medium consisting of 1/2-strength macro and micro elements and all of the vitamins described by Murashige & Skoog (1962), 20 g/L sugar, and 6.5 g/L agar (Men et al 2003). Capsules of D. candidum were collected from Xishuanbanna, Yunnan, China (November, 2010) and germinating seeds at the stage 1-2 (Stewart et al 2003) were obtained according to the designated procedures (Wang et al 2011). All plant materials were kept in a high-humidity (75 ± 5%) chamber at 25±2…”

Section: Plant Materials and Samplingsmentioning

confidence: 99%

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

et al. 2012

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AbstractCyclin-dependent kinases (CDKs) play an essential role in cell cycle regulation during the embryonic and postembryonic development of organisms. To better understand the molecular mechanisms of CDKs involved in embryogenesis regulation in the endangered medicinal plant Dendrobium candidum Wall. ex Lindl., a 1229-bp full-length cDNA of an A-type CDK gene, Denca;CDKA;1, was identified using 3′ rapid amplification of cDNA end (RACE) PCR. Denca;CDKA;1 was predicted to encode a 294 amino acid residue-long protein of 33.76 kDa with an isoelectric point of 7.72. The deduced Denca;CDKA;1 protein contained a conserved serine/threonine-protein kinase domain (S-TKc) and a canonical cyclinbinding “PSTAIRE” motif. Multiple sequence alignment indicated that members of CDKA family from various plants exhibited a high degree of sequence identity ranging from 82% to 93%. A neighbor-joining phylogenetic tree showed that Denca;CDKA;1 was clustered into the plant group and was distant from the animal and fungal groups. The modeled three-dimensional structure of Denca;CDKA;1 exhibited the similar functional structure of a fold consisting of β-sheets and α-helices joined by discontinuous random coils forming two relatively independent lobes. Quantitative real-time PCR analysis revealed that Denca;CDKA;1 transcripts were the most abundant in protocorm-like bodies with 4.76 fold, followed by that in roots (4.19 fold), seeds (2.57 fold), and stems (1.57 fold). This study characterized the novel Denca;CDKA;1 gene from D. candidum for the first time and the results will be useful for further functional determination of the gene.

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In situ seed baiting techniques in Dendrobium officinale Kimuraet Migo and Dendrobium nobile Lindl. : the endangered Chinese endemic Dendrobium (Orchidaceae)

Cited by 47 publications

References 16 publications

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium (Orchidaceae)

ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

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