In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM

Ahmad, Mohd Ridzuan; Nakajima, Masahiro; Kojima, Seiji; Homma, Michio; Fukuda, Toshio

doi:10.1109/mhs.2007.4420816

Cited by 11 publications

(24 citation statements)

References 42 publications

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“…In general, the fermentation process of the yeast decreases the average surface roughness from 0.32 μm to 0.22 μm ( Fig. 2a,b ) accordingly to the yeast morphology reported elsewhere 21 . Representative images of the samples (see Fig.…”

Section: Resultssupporting

confidence: 78%

“…4a the experimental curve slope and fracture strength (see also Supplementary Fig. S5 ) are lower than the cell elastic parameters reported in literature 21 30 , thus we deduce that the composite failure occurs due to interface rupture. The curve was then used to determine the yeast interface fracture energy that is estimated to be G f-yeast = 0.0193 N/m assuming a cell diameter, and then distance from interfaces, of 5 μm.…”

Section: Resultsmentioning

confidence: 48%

“…The dimension of the cell, mother and daughter, and their elastic properties as well, were taken from the work of Ahmad et al . 21 They report for the mother cell a diameter d m = 5.565 μm and Young’s modulus E m = 1.46 MPa, while for the daughter cell d d = 4.467 μm and Young’s modulus E d = 1.10 MPa. The cell dimensions reported in this work are consistent with our estimations (see Fig.…”

Section: Resultsmentioning

confidence: 98%

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Fermentation based carbon nanotube multifunctional bionic composites

Valentini

Bon

Signetti

et al. 2016

Sci Rep

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The exploitation of the processes used by microorganisms to digest nutrients for their growth can be a viable method for the formation of a wide range of so called biogenic materials that have unique properties that are not produced by abiotic processes. Here we produced living hybrid materials by giving to unicellular organisms the nutrient to grow. Based on bread fermentation, a bionic composite made of carbon nanotubes (CNTs) and a single-cell fungi, the Saccharomyces cerevisiae yeast extract, was prepared by fermentation of such microorganisms at room temperature. Scanning electron microscopy analysis suggests that the CNTs were internalized by the cell after fermentation bridging the cells. Tensile tests on dried composite films have been rationalized in terms of a CNT cell bridging mechanism where the strongly enhanced strength of the composite is governed by the adhesion energy between the bridging carbon nanotubes and the matrix. The addition of CNTs also significantly improved the electrical conductivity along with a higher photoconductive activity. The proposed process could lead to the development of more complex and interactive structures programmed to self-assemble into specific patterns, such as those on strain or light sensors that could sense damage or convert light stimulus in an electrical signal.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 98%

See 1 more Smart Citation

Fermentation based carbon nanotube multifunctional bionic composites

Valentini

Bon

Signetti

et al. 2016

Sci Rep

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show abstract

“…This could be an advantage by integrating tools such as nanomanipulators or patch clamp to analyze cells and their characteristics while performing experiments. From our previous work, a nanomanipulator was successfully incorporated inside ESEM ( Figure 1 ) for various single cell manipulation and characterization [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Environmental Scanning Electron Microscope-Nanomanipulation System on Spheroplast Yeast Cells Surface Observation

et al. 2017

Self Cite

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The preparation and observations of spheroplast W303 cells are described with Environmental Scanning Electron Microscope (ESEM). The spheroplasting conversion was successfully confirmed qualitatively, by the evaluation of the morphological change between the normal W303 cells and the spheroplast W303 cells, and quantitatively, by determining the spheroplast conversion percentage based on the OD800 absorbance data. From the optical microscope observations as expected, the normal cells had an oval shape whereas spheroplast cells resemble a spherical shape. This was also confirmed under four different mediums, that is, yeast peptone-dextrose (YPD), sterile water, sorbitol-EDTA-sodium citrate buffer (SCE), and sorbitol-Tris-Hcl-CaCl2 (CaS). It was also observed that the SCE and CaS mediums had a higher number of spheroplast cells as compared to the YPD and sterile water mediums. The OD800 absorbance data also showed that the whole W303 cells were fully converted to the spheroplast cells after about 15 minutes. The observations of the normal and the spheroplast W303 cells were then performed under an environmental scanning electron microscope (ESEM). The normal cells showed a smooth cell surface whereas the spheroplast cells had a bleb-like surface after the loss of its integrity when removing the cell wall.

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“…Also atomic force microscope (AFM) method has been performed by several researchers [14], although positioning of AFM probe and probe-to-cell attachment are not easily conducted. There are some other methods for investigating the mechanical properties of cells, such as micro fluidic cell method [15,16] and a microtool method [17,18]. However, these methods are for cell level observation, and inappropriate for local mechanical property measurement.…”

Section: Introductionmentioning

confidence: 99%

Cell palpation with an optically trapped particle

Sugiura

Miyoshi

Nishio

et al. 2012

J. Micro-Nano Mech.

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We have developed a cell palpation system to investigate cell stiffness from the reaction force generated on a particle that is fixed on a cell. In this method, a particle is used as a probe, and is manipulated towards a cell using optical tweezers. Using this method, we can obtain information of local stiffness of a cell. We investigate focal adhesion formation of a cell probed by a particle and we report different particle coating utilized for attaching certain protein in cell membrane. Also we discuss the effects of endocytosis.

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In-situ Single Cell Mechanics Characterization of W303 Yeast Cells using Nanoneedles inside Environmental-SEM

Cited by 11 publications

References 42 publications

Fermentation based carbon nanotube multifunctional bionic composites

Fermentation based carbon nanotube multifunctional bionic composites

Application of Environmental Scanning Electron Microscope-Nanomanipulation System on Spheroplast Yeast Cells Surface Observation

Cell palpation with an optically trapped particle

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