In Situ Synthesis of Silver Nanoparticles on the Polyelectrolyte-Coated Sericin/PVA Film for Enhanced Antibacterial Application

Abstract

To develop silk sericin (SS) as a potential antibacterial biomaterial, a novel composite of polyelectrolyte multilayers (PEMs) coated sericin/poly(vinyl alcohol) (SS/PVA) film modified with silver nanoparticles (AgNPs) has been developed using a layer-by-layer assembly technique and ultraviolet-assisted AgNPs synthesis method. Ag ions were enriched by PEMs via the electrostatic attraction between Ag ions and PEMs, and then reduced to AgNPs in situ with the assistance of ultraviolet irradiation. PEMs facilitated the high-density growth of AgNPs and protected the synthesized AgNPs due to the formation of a 3D matrix, and thus endowed SS/PVA film with highly effective and durable antibacterial activity. Scanning electron microscopy, energy dispersive spectroscopy, X-ray diffractometry, Fourier transfer infrared spectroscopy, water contact angle, mechanical property and thermogravimetric analysis were applied to characterize SS/PVA, PEMs-SS/PVA and AgNPs-PEMs-SS/PVA films, respectively. AgNPs-PEMs-SS/PVA film has exhibited good mechanical performance, hydrophilicity, water absorption capability as well as excellent and durable antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa and good stability and degradability. This study has developed a simple method to design and prepare AgNPs-PEMs-SS/PVA film for potential antibacterial application.

Polydopamine-Assisted Silver Nanoparticle Self-Assembly on Sericin/Agar Film for Potential Wound Dressing Application

“…Silkworm cocoons were cut into pieces and treated at 121 °C and 0.1 MPa for 15 min. Then, sericin was extracted into solution and separated from silk fibroin by filtration, which is descripted in our previous report [ 41 , 42 , 43 ]. Agar was dissolved in water with agitation at 90 °C to a final concentration of 2% ( w/v ).…”

Fabrication of Sericin/Agrose Gel Loaded Lysozyme and Its Potential in Wound Dressing Application

“…The swelling ability of the SS/AR gel was analyzed using a conventional gravimetric method [ 32 ]. Briefly, a pre-weighed dry gel (Wd) was immersed into water at 37 °C for 30 min to achieve equilibrium.…”

Photothermally Active Inorganic Nanoparticles: from Colloidal Solutions to Photothermally Active Printed Surfaces and Polymeric Nanocomposite Materials

“…During the preparation of these nanocomposites, the nanoparticles can be directly blended together with polymers, deposited on the top of material surface, or in‐situ synthesized in the polymeric matrix. In the latter case, many approaches were reported in the literature: in‐situ generation of silver nanoparticles in poly(vinyl alcohol film),, in‐situ synthesis of gold nanoparticles – poly(dimethylsiloxane) nanocomposites and in situ reduction of gold(III) chloride inside a hydrogel network, or in situ synthesis of silver and gold nanoparticles inside polymer films by mild thermal annealing . Nanoparticles displaying particular functional groups can also act as cross‐linkers of polymer networks …”