In-syringe dispersive solid phase filter extraction cleanup followed by liquid chromatography-triple quadrupole mass spectrometry for fast determination of colchicine in plasma/urine

Qian, Mingrong; Chen, Zhimin; Tao, Xue-xin; Feng, Yao; Xu, Xia

doi:10.1016/j.jpba.2023.115317

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The proposed method demonstrated a linearity range of 0.04-20 ng/mL and limits of detection (LOD) and quantitation (LOQ) of 0.06 ng/mL and 0.2 ng/mL for colchicine in plasma and urine. Mean extraction yields at three different spiking levels in plasma and urine are quite reproducible (93.9-102.68%), with acceptable relative standard deviation (RSD) values [24]. Moreover, the investigation thoroughly validates the analytical approach by assessing matrix effect, stability, dilution integrity, and carryover.…”

Section: Solid-phase-based Extraction Proceduresmentioning

confidence: 67%

“…The approach, also known as dispersive micro-solid phase extraction (D-µ-SPE), utilizes small quantities (milligrams) of sorbent. Qian et al [24] proposed a fast and extremely precise approach for reliable colchicine measurement in plasma and urine. The method involves a combination of in-syringe d-SPE and LC-QqQ with ESI (d-SPE-ESI-LC-MS/MS) in MRM mode, demonstrating very good linearity and accuracy at therapeutic drug levels.…”

Section: Solid-phase-based Extraction Proceduresmentioning

confidence: 99%

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Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples

Mahdavijalal,

Petio,

Staffilano

et al. 2024

Molecules

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In the past few decades, considerable scientific strides have been made in the subject of drug analysis in human biological samples. However, the risk caused by incorrect drug plasma levels in patients still remains an important concern. This review paper attempts to investigate the advances made over the last ten years in common sample preparation techniques (SPT) for biological samples based on solid sorbents, including solid-phase extraction (SPE) and solid-phase micro-extraction (SPME), and in particular in the field of molecularly imprinted polymers (MIPs), including non-stimuli-responsive and stimuli-responsive adsorbents. This class of materials is known as ‘smart adsorbents’, exhibiting tailored responses to various stimuli such as magnetic fields, pH, temperature, and light. Details are provided on how these advanced SPT are changing the landscape of modern drug analysis in their coupling with liquid chromatography-mass spectrometry (LC-MS) analytical techniques, a general term that includes high-performance liquid chromatography (HPLC) and ultra-high performance liquid chromatography (UHPLC), as well as any variation of MS, such as tandem (MS/MS), multiple-stage (MSn), and high-resolution (HRMS) mass spectrometry. Some notes are also provided on coupling with less-performing techniques, such as high-performance liquid chromatography with ultraviolet (HPLC-UV) and diode array detection (HPLC-DAD) detection. Finally, we provide a general review of the difficulties and benefits of the proposed approaches and the future prospects of this research area.

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Section: Solid-phase-based Extraction Proceduresmentioning

confidence: 67%

Section: Solid-phase-based Extraction Proceduresmentioning

confidence: 99%

Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples

Mahdavijalal,

Petio,

Staffilano

et al. 2024

Molecules

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“…The primary objective of this study was to establish a rapid and effective quantitation method for DIZ, TOZ, TZSO, TZS, and NICA in chicken muscle and eggs. In this study, we used an in-syringe DSPE, a widely utilized technique for rapid sample pretreatment [ 21 , 22 ]. The whole procedure is easy to operate and fast, involving extraction with ACN, the addition of NaCl to remove large amounts of protein from the samples, followed by the addition of a suitable sorbent and further n-hexane to eliminate both polar and fat-soluble interferences in the samples.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Analysis of Nicarbazin, Diclazuril, Toltrazuril, and Its Two Metabolites in Chicken Muscle and Eggs by In-Syringe Dispersive Solid-Phase Filter Clean-Up Followed by Liquid Chromatography–Tandem Mass Spectrometry

An,

Pan,

Cai

et al. 2024

Foods

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Nicarbazin (NICA) and triazine anticoccidial drugs (diclazuril (DIZ) and toltrazuril (TOZ)) are the primary strategy for preventing and treating coccidiosis. To prevent the development of drug resistance and mitigate the potential chronic toxicity to humans resulting from prolonged exposure, a liquid chromatography–tandem mass spectrometry method with high reliability and sensitivity was developed to determine NICA, DIZ, TOZ, and its two metabolites in chicken muscle and eggs. Upon establishing the extraction conditions involving 10 mL of acetonitrile and 10 min of sonication, in-syringe dispersive solid-phase extraction with silica was performed in combination with n-hexane clean-up. The selection of isotope peaks of precursor ions and low-mass range scanning allowed the two transitions for the quantification of all compounds. The limits of detection for DIZ and NICA were both 0.1 μg/kg, and for TOZ and metabolites, they were 0.3 μg/kg; the limits of quantitation were 0.3 and 1 μg/kg, respectively. The linear range was 0.25–50 ng/mL with a correlation coefficient r > 0.999. The average recoveries at three spiking levels in muscle and eggs were 90.1–105.2% and 94.0–103.7% with the relative standard deviations of 3.0–8.1% and 3.1–14.4%, respectively. The precision, accuracy, and stability were evaluated by three quality control samples.

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Colchicine overdose and misuse

2023

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In-syringe dispersive solid phase filter extraction cleanup followed by liquid chromatography-triple quadrupole mass spectrometry for fast determination of colchicine in plasma/urine

Cited by 4 publications

References 25 publications

Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples

Innovative Solid-Phase Extraction Strategies for Improving the Advanced Chromatographic Determination of Drugs in Challenging Biological Samples

Simultaneous Analysis of Nicarbazin, Diclazuril, Toltrazuril, and Its Two Metabolites in Chicken Muscle and Eggs by In-Syringe Dispersive Solid-Phase Filter Clean-Up Followed by Liquid Chromatography–Tandem Mass Spectrometry

Colchicine overdose and misuse

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