In syringe hybrid monoliths modified with gold nanoparticles for selective extraction of glutathione in biological fluids prior to its determination by HPLC

Mompó-Roselló, Óscar; Vergara-Barberán, María; Simó‐Alfonso, Ernesto Francisco; Herrero‐Martínez, José Manuel

doi:10.1016/j.talanta.2019.120566

Cited by 23 publications

(10 citation statements)

References 30 publications

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“…As shown in Figure a, the recoveries of GSH and Hcy increased with pH from 4 to 5. This was due to the reason that the H + concentration increased along with the decrease in pH value, and the carboxyl group of glutathione was protonated, which formed strong hydrogen bonds between aminothiol molecules and facilitated strong electrostatic repulsion with SiO 2 @50Benz-Cys-Hg + . On the other hand, the extraction recoveries for both GSH and Hcy significantly decreased with the increase in pH in the range of 5–9.…”

Section: Resultsmentioning

confidence: 99%

“…Sample Extraction and Pretreatment. The pre-processing method referred to the report of Mompu-Rosellúet al 20 with a little modification. After being unfrozen, 100 μL of urine sample was mixed with 10 μL of TCEP solution (50 mmol L −1 in borate buffer, pH 7.4) in a tube and then incubated at 25 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…This was due to the reason that the H + concentration increased along with the decrease in pH value, and the carboxyl group of glutathione was protonated, which formed strong hydrogen bonds between aminothiol molecules and facilitated strong electrostatic repulsion with SiO 2 @ 50Benz-Cys-Hg + . 20 On the other hand, the extraction recoveries for both GSH and Hcy significantly decreased with the increase in pH in the range of 5−9. It was reported that the zwitterionic forms of these aminothiol molecules were usually required to achieve their maximum adsorption amount on the material surface.…”

Section: Optimized Conditions Of the Extractionmentioning

confidence: 99%

“…As shown in Table 1, the developed methods established the LOD at 0.5 nmol L −1 for GSH and 1 nmol L −1 for Hcy, which were notably lower than those reported previously. 20 Compared with the determination of derivatized aminothiols, this strategy can offer better selectivity and sensitivity toward underivatized aminothiols (Table S3). In virtue of the good sensitivity and accuracy, the proposed method can be an important alternative for selective extraction and enrichment of low-concentration aminothiols in biological samples.…”

Section: Optimized Conditions Of the Extractionmentioning

confidence: 99%

“…Gold nanoparticles are commonly used as adsorbents for GSH and Hcy in light of their good biocompatibility and affinity for compounds containing thiol groups . However, high cost, lack of selectivity, and additional derivatization procedures are the main disadvantages during the process of fluorescence detection. − However, in the derivatization procedure, the complicated pretreatment steps hardly prevent the oxidation and degradation of the analyte. Especially in biological samples such as urine and saliva, the content of such analytes is extremely low.…”

Section: Introductionmentioning

confidence: 99%

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Targeting Enrichment and Correlation Studies of Glutathione and Homocysteine in IgAVN Patient Urine Based on a Core–Shell Zr-Based Metal–Organic Framework

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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Aminothiols are closely related to chronic kidney disease, but little is known regarding levels of related aminothiols in the urine of immunoglobulin A vasculitis with nephritis (IgAVN) patients. Herein, a well-defined core−shell Zr-based metal−organic framework (Zr-MOF) composite SiO 2 @50Benz-Cys was constructed as a mercury ion affinity material via a solvent-assisted ligand exchange strategy for the selective extraction and enrichment of low-concentration aminothiols in IgAVN patient urine. SiO 2 @50Benz-Cys was competent to enrich the total glutathione (GSH) and total homocysteine (Hcy) in virtue of the excellent affinity after chelation with mercury ions. The extraction efficiencies were closely related to the pH, dithiothreitol amount, and the dose of functional Zr-MOF. Coupled with HPLC−MS/MS in optimized conditions, GSH and Hcy were determined with low detection limits of 0.5 and 1 nmol L −1 , respectively. The recoveries of GSH and Hcy for the urine sample at three spiked levels were in the range of 85.3−105% and 79.5−103%, which showed good precision and accuracy. Benefiting from the matrix interference elimination in the process of extraction, the simultaneous detection of aminothiols in the urine of the healthy group and immunoglobulin A vasculitis (IgAV) and IgAVN patients was successfully carried out, suggesting that the Zr-MOF and the robust method together provided a potential application in the analysis of urinary biomolecules. The analysis of variance (ANOVA) showed that the levels of GSH and Hcy had significant differences between the patients and the control. This work is very valuable as it provides a better understanding of concentration alterations of GSH and Hcy in urine involved with IgAVN for clinical research.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Optimized Conditions Of the Extractionmentioning

confidence: 99%

Section: Optimized Conditions Of the Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Targeting Enrichment and Correlation Studies of Glutathione and Homocysteine in IgAVN Patient Urine Based on a Core–Shell Zr-Based Metal–Organic Framework

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

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Recent developments and applications of polymer monolithic stationary phases

Eeltink

Meston

Švec

2021

Analytical Science Advances

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This review highlights the current trends and the most recent advances in the field of preparation and application of organic polymer‐based monolithic materials and covers literature published in 2020. A short historical background is provided and protocols to anchor monoliths covalently to the wall of the column/separation device are discussed. Furthermore, advances in tuning the macroporous structure and establishing its link to separation performance are conferred. Finally, method development and key applications using novel monolithic columns are discussed.

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Use of metal nanoparticles for preconcentration and analysis of biological thiols

2022

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Metal nanoparticles (NPs) exhibit several unique physicochemical properties, including redox activity, surface plasmon resonance, ability to quench fluorescence, biocompatibility, or a high surface‐to‐volume ratio. They are being increasingly used in analysis and preconcentration of thiol containing compounds, because they are able to spontaneously form a stable Au/Ag/Cu–S dative bond. They thus find wide application in environmental and particularly in medical science, especially in the analysis of biological thiols, the endogenous compounds that play a significant role in many biological systems. In this review article, we provide an overview of various types of NPs that have been applied in analysis and preconcentration of biological thiols, mainly in human biological fluids. We first discuss shortly the types of NPs and their synthesis, properties, and their ability to interact with thiol compounds. Then we outline the sample preconcentration and analysis methods that were used for this purpose with special emphasis on optical, electrochemical, and separation techniques.

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In syringe hybrid monoliths modified with gold nanoparticles for selective extraction of glutathione in biological fluids prior to its determination by HPLC

Cited by 23 publications

References 30 publications

Targeting Enrichment and Correlation Studies of Glutathione and Homocysteine in IgAVN Patient Urine Based on a Core–Shell Zr-Based Metal–Organic Framework

Targeting Enrichment and Correlation Studies of Glutathione and Homocysteine in IgAVN Patient Urine Based on a Core–Shell Zr-Based Metal–Organic Framework

Recent developments and applications of polymer monolithic stationary phases

Use of metal nanoparticles for preconcentration and analysis of biological thiols

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