In utero exposure to perfluorooctanoate (PFOA) or perfluorooctane sulfonate (PFOS) did not increase body weight or intestinal tumorigenesis in multiple intestinal neoplasia (Min/+) mice

Abstract

We examined whether perfluorooctanoate (PFOA) or perfluorooctane sulfonate (PFOS) had obesogenic effects and if they increased spontaneous intestinal tumorigenesis in the mouse model C57BL/6J-Min/+ (multiple intestinal neoplasia) after in utero exposure. The dams were exposed to PFOA or PFOS (0.01, 0.1 or 3.0 mg/kg bw/day) by po gavage on GD1-17. TheMin/+ and wild-type offspring were terminated at week 11 for examination of intestinal tumorigenesis or at week 20 for obesogenic effect, respectively. Body weights of the dams and pups were recorded throughout life. Food intake was determined at week 6 and 10. Blood glucose (non-fasted) was measured at week 6 and 11. No obesogenic effect of PFOA or PFOS was observed up to 20 weeks of age. PFOA or PFOS did not increase the incidence or number of tumors in the small intestine or colon of the Min/+ mice or affect their location along the intestines. Feed intake was not affected. There were some indications of toxicity of PFOA, but not of PFOS. There was lower survival of pups after 3.0 mg/kg PFOA, lower body weight in pups after 3.0 and possibly 0.1 mg/kg PFOA, and increased relative liver weight after 0.01 and possibly 0.1 mg/kg PFOA. Plasma glucose was lower after 0.01 and 0.1 mg/kg PFOA. In conclusion, exposure to PFOA and PFOS in utero with the doses used did not have obesogenic effect on either Min/+ or wild-type mice, at least not up to 11 or 20 weeks of age, nor increased intestinal tumorigenesis in Min/+ mice.

Effects of Periconception Cadmium and Mercury Co-Administration to Mice on Indices of Chronic Diseases in Male Offspring at Maturity

“…In the present studies, due to potential differences in susceptibility of adverse in utero conditions between each sibling within a litter, individual offspring were used as an experimental unit (Chen et al 2015; Ngo et al 2014). Effects on birth weights, litter size, organ weights, area under the curve, hormone concentrations, differences in mRNA expression, and open arm entries in the EPM were analyzed using a one-way ANOVA with Proc GLM in SAS (version 9.2; SAS Institute, Cary, NC, USA).…”

Metabolism disrupting chemicals and metabolic disorders

“…For example, prenatal exposure to perfluorinated compounds, chemicals used to repel grease stains in carpets and clothing, was not associated with subsequent adiposity in childhood [396] in a recent study, though a prior study found such an association at age 20y [397]. Similarly, in animal models, one study found in utero exposure to perfluorooctanoic acid (PFOA) led to weight gain in offspring in outbred CD-1 mice [398] whereas another inbred transgenic mouse model did not show any weight gain [399].…”

Prenatal perfluoroalkyl substance exposure and child adiposity at 8 years of age: The HOME study

“…13 Four prospective epidemiological studies have examined the relationship between early life PFAS exposure and child or adult adiposity (Table S1). 14–17 Two studies reported no associations between prenatal or early childhood PFAS exposure and child or adult adiposity.…”

“…Some experimental rodent and in vitro studies show that PFAS exposure may cause impaired glucose homeostasis, increased body weight, and altered adipocyte differentiation (9)(10)(11)(12), while others do not (13). Four prospective epidemiological studies have examined the relationship between early life PFAS exposure and child or adult adiposity (Supporting Information Table S1) (14)(15)(16)(17).…”