In vitro activity and Cytotoxicity of Crocus sativus Extract against Leihmania Major (MRHO/IR/75/ER)

Yousefi, Elham; Eskandari, Alborz; Gharavi, Mohammad Javad; Khademvatan, Shahram

doi:10.2174/1871526514666140827101901

Cited by 28 publications

(18 citation statements)

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“…C . sativus and its bio‐active compounds have also been indicated to possess anticancer (Tseng et al ., ; Wang et al ., ) anti‐microbial (Yousefi et al ., ), anti‐mutagenic (Bhandari, ), and anti‐genotoxic (Premkumar et al ., ) activities. The therapeutic effects of C .…”

Section: Introductionmentioning

confidence: 99%

Antiinflammatory, Antioxidant, and Immunomodulatory Effects ofCrocus sativusL. and its Main Constituents

2016

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Crocus sativus L. (C. sativus), commonly known as saffron, is used as a food additive, preservative, and medicinal herb. Traditionally, it has been used as an alternative treatment for different diseases. C. sativus' medicinal effects are related to its major constituents like crocins, crocetin, and safranal. According to the literature, C. sativus and its constituents could be considered as an effective treatment for neurodegenerative disorders, coronary artery diseases, asthma, bronchitis, colds, fever, diabetes, and so on. Recently, numerous studies have reported such medicinal properties and found that the underlying mechanisms of action may be mediated by antioxidant, inflammatory, and immunomodulatory effects. C. sativus enhances the antioxidant capacity and acts as a free radical scavenger. As an antiinflammatory and immunomodulatory agent, it modulates inflammatory mediators, humoral immunity, and cell-mediated immunity responses. This review highlights in vitro and animal findings regarding antiinflammatory, antioxidant, and immunomodulatory effects of C. sativus and its constituents. Present review found that the C. sativus and its main constituents such as safranal, crocins, and crocetin could be effective against various diseases because of their antioxidant, anti-inflammation, and immunomodulatory effects. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Antiinflammatory, Antioxidant, and Immunomodulatory Effects ofCrocus sativusL. and its Main Constituents

2016

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“…nested-PCR technique was utilized to amplify a fragment of SSU 18s rRNA using 2 sets of primers as follow: (F1): 5' -TTCTAGAGCTAATACATGCG -3' and (R1): 5' -CCCATTTCCTTCGAAACAGGA -3' for first stage of PCR and (F2): 5' -GGAAGGGTTGTATTTATTAGATAAAG-3' and (R2): 5' -CTCATAAGG TGCTGAAGGAGTA -3' for secondary PCR (7,9,19,20). The PCR products were loaded on 1.5% agarose gel and visualized under UV light using Gel Doc device (Uvidoc, Gel Documentation System, and Cambridge, UK) (12,13,21,22).…”

Section: Dna Extraction and Nested-pcrmentioning

confidence: 99%

Molecular Characterization of Cryptosporidium Species Isolated from Cattle in Southwest of Iran

Saki

Asadpouri

2017

Jundishapur J Microbiol

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Background: Cryptosporidium spp. is an ubiquitous intracellular protozoan parasite that affect a wide range of vertebrates like humans and livestock. Objectives: The current survey was aimed to determine the prevalence and molecular characterization of Cryptosporidium spp. in cattle of Ahvaz city, southwest of Iran. Methods: In total, 240 cattle fecal specimens were collected from 5 geographical regions of Ahvaz city and microscopically tested for the presence of Cryptosporidium spp. oocysts. Then, all positive samples were examined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) to detect and genotyping of Cryptosporidium spp., respectively. Finally, the obtained results were sequenced. Results: The overall prevalence of Cryptosporidium infection by modified Ziehl-Neelsen staining and nested-PCR methods were 2.1% (5/240) in cattle. Digestion of secondary PCR products using the RFLP method and employing SspI and VspI enzymes, revealed C. parvum pattern in all positive cases and also confirmed by sequencing. Conclusions: Current finding suggests that C. parvum is the main species in cattle of Ahvaz city and could be considered as an important reservoir for zoonotic infection.

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“…In many developing countries, people pay special attention to traditional medicines and use them to meet their health needs. Given that herbal medicines often do not have any side effects and are available and cheap, the importance of using native herbs in each region is highlighted (9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

The In Vitro Effect of Hydroalcoholic Extract of Artemisia absinthium on the Growth of Leishmania major (MRHO/IR/75/ER) in Peritoneal Macrophages from BALB/c Mice

Rahiminejad¹,

Tappeh²,

Seyyedi³

et al. 2018

Jundishapur J Microbiol

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Background: Leishmaniasis is a protozoan disease ranging from simple to mucocutaneous, diffuse, and visceral lesions. Current chemical medicines, including Glucantime, have high toxicity, side effects, and drug resistance. Herbal medicines are unlimited sources for discovering new medications to treat infectious diseases. Objectives: This study aimed to examine the in vitro effect of hydroalcoholic extract of Artemisia absinthium on the growth of Leishmania major in peritoneal macrophages from BALB/c mice. Methods: In this experimental study, the hydroalcoholic extract of A. absinthium was prepared and dissolved in 2% dimethyl sulfoxide (DMSO). Leishmania major (MRHO/IR/75/ER) was cultured in RPMI1640 medium containing 15% fetal bovine serum (FBS). Peritoneal macrophages were extracted from BALB/c mice and were infected with promastigotes at a ratio of 1:10. The extract was added to Leishmania-infected macrophages and promastigotes at different concentrations. After 24, 48, and 72 hours, the effectiveness of A. absinthium on promastigotes was assessed using Trypan blue test and methyl thiazole tetrazolium (MTT) colorimetric assay, and the half maximal inhibitory concentration (IC50) of A. absinthium extract was obtained. Giemsa staining was used to evaluate the effect of A. absinthium extract on amastigotes. Also, inducible nitric oxide synthase (iNOs) level was measured from Leishmania-infected macrophages. The data were analyzed by ANOVA in SPSS software. Results: The hydroalcoholic extract of A. absinthium showed a significant anti-Leishmania activity against promastigotes and amastigotes at various concentrations after 48 and 72 hours. The IC50 values of the alcoholic extract of A. absinthium were obtained at 56 mg/mL and 51 mg/mL concentrations of promastigotes and amastigotes, respectively. The lowest levels of iNOs were seen at 100 and 50 mg/mL concentrations of A. absinthium in treated macrophages. The former was obtained at 60.8 and 58.8 ng/mL after 24 and 48 hours and the latter at 55.5 ng/mL and 50 mg/mL of A. absinthium, respectively, after 72 hours. Conclusions: Findings of the present study showed the anti-parasitic effects of A. absinthium on both promastigotes and amastigotes. Further studies are recommended to be conducted on animal models and the active ingredients of A. absinthium extract to accurately assess the effects of this extract on the growth of L. major.

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In vitro activity and Cytotoxicity of Crocus sativus Extract against Leihmania Major (MRHO/IR/75/ER)

Cited by 28 publications

References 0 publications

Antiinflammatory, Antioxidant, and Immunomodulatory Effects ofCrocus sativusL. and its Main Constituents

Antiinflammatory, Antioxidant, and Immunomodulatory Effects ofCrocus sativusL. and its Main Constituents

Molecular Characterization of Cryptosporidium Species Isolated from Cattle in Southwest of Iran

The In Vitro Effect of Hydroalcoholic Extract of Artemisia absinthium on the Growth of Leishmania major (MRHO/IR/75/ER) in Peritoneal Macrophages from BALB/c Mice

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