In vitro adventitious shoot regeneration from leaf explants of some apricot cultivars

Abstract

Apricot is one of the most valuable commercial fruits. In vitro propagation of apricot is very important for rapid multiplication of cultivars with desirable traits and production of cleaning up and virus-free plants. Low frequency of multiplication is the main limiting factor for traditional propagation methods. In this regard, the objective of our investigation was to study the morphogenetic capacity of apricot leaf explants of the promising cultivars ‘Iskorka Tavridy’, ‘Magister’ and ‘Bergeron’ for regeneration system development and solving some breeding questions. The source of explants was in vitro plants regenerated and cultured on QL medium. Leaves were maintained in the dark at 24±1 °C in thermostat for three-four weeks. Morphogenic callus and structures were mainly formed at the central and proximal parts of leaves on MS, QL and WPM media with 1.5 or 2.0 mg L-1 BAP and 1.5 or 2.0 mg L-1 IAA in different combinations, or TDZ (0.6 and 1.3 mg L-1). Callus with adventive buds was transferred to regeneration medium and placed into a growth chamber at 24±1 °C and 16-hour photoperiod with a light intensity of 37.5 μmol m-2 s-1. The best results were obtained when adaxial leaf surface was in contact with the culture medium. Frequency of leaf callus formation on MS medium with 1.5 mg L-1 BAP and 1.5 mg L-1 IAA was higher in the explants of ‘Iskorka Tavridy’ (80.0%) than in - ‘Bergeron’ (50.0%) and ‘Magister’ (36.7%). The best results of callogenesis for ‘Magister’ was obtained on MS medium with 1.3 mg L-1 TDZ (53.3%). Active microshoot regeneration in ‘Iskorka Tavridy’ cultivar was shown on MS medium with BAP and IAA and in ‘Magister’ cultivar - on MS medium with TDZ. Rhizogenesis was obtained on half strength MS medium with 2.0 mg L-1 IBA.

Comparative Studies of In Vitro Regeneration Capacity in Some Breeding Forms of Prunus persica (L.) Batsch

“…Biotechnological methods were used in the presented research [8,11]. Morphogenesis induction and regenerative capacity of peach explants were studied on Murashige, Skoog (MS) [12] and Gamborg, Eveleigh (B5) [13] culture media supplemented with vitamins and plant growth regulators.…”

“…In this regard, an effective and reliable system of peach microshoots regeneration from somatic tissues under in vitro conditions is necessary [7,8]. It is known that peach is one of the crops to most difficult propagate under in vitro conditions [6,9,10]. The aim of the presented study was to identify morphogenetic capacity of organs and tissues in peach hybrid forms under in vitro conditions for further studies and use in the breeding process.…”

In vitro introduction of Prunus persica (L.) Batsch ‘Dostoyniy’

“…Among them, 288 cultivars were bred in the NBG [7]. For some native ('Posol Mira̕ , 'Rodzynka̕ , 'Zolotaya Moskva, 'Sovetskiy̕ , 'Pamyatniy Nikitskiy̕ , 'Lakomy̕ , 'Orfei̕ ) and foreign cultivars (Ambergold̕ , 'Dixired̕ , 'Red Haven̕ ) microshoot regeneration in vitro was performed [8,9]. Under cross-breeding of 'Zlatogor̕ and 'Uspar̕ , performed by Z.N.…”