In vitro and ex vitro propagation of Stevia rebaudiana Bertoni with high Rebaudioside-A content—A commercial scale application

Yücesan, Buhara; Mohammed, Aliyu; Büyükgöçmen, Refik; Altuğ, Cevher; Kavas, Özge; Gürel, Songül; Gürel, Ekrem

doi:10.1016/j.scienta.2016.03.008

Cited by 26 publications

(8 citation statements)

References 26 publications

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“…Moreover, the study of ZAYOVA et al (2013) demonstrated that a medium with the addition of BAP at 1 mg•dm -3 concentration enables the production of a higher number of shoots in stevia than at 0.5 mg•dm -3 concentration. YÜCESAN et al (2016) demonstrated that addition of BAP and KIN at concentrations between 0.1 -2 mg•dm -3 results in the development of an equal number of shoots, yet the longest shoots were obtained when higher KIN concentrations were used (0.5, 1 and 2 mg•dm -3 ).…”

Section: Propagation Stagementioning

confidence: 95%

Micropropagation of Stevia rebaudiana plants

Rokosa

Kulpa

2020

Cienc. Rural

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The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.

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Section: Propagation Stagementioning

confidence: 95%

Micropropagation of Stevia rebaudiana plants

Rokosa

Kulpa

2020

Cienc. Rural

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“…López et al (2016) also reported that IBA at 1.0 ppm produces higher plants and longer roots in stevia [14]. Moreover, other studies have reported that other hormones, such as IAA at a concentration of 0.25 mg/L or ANA at 0.25 or 0.50 mg/L, also help in the formation of stevia roots [22]. ANA has been reported to stimulate cell elongation, growth, division, and differentiation, as well as to regulate abscission and stimulate the growth of adventitious roots [23].…”

Section: Growth and Development Of Stevia Plants In Different Months mentioning

confidence: 97%

Growth and Development of Stevia Cuttings During Propagation with Hormones in Different Months of the Year

Castañeda-Saucedo

Tapia‐Campos

Ramírez-Anaya

et al. 2020

Plants

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Stevia is an important non-caloric sweetener that has health-beneficial properties. The objective is to evaluate growth, development, and rooting of stevia plants during different seasons of the year using growth hormones. Eight experiments were set up in Ciudad Guzman, Jalisco, Mexico, with three treatments (T): T1, indol-3 butyric acid (IBA) 7.4 mM; T2, alphanaphthylacetamide (ANA) 6.4 mM + IBA 0.3 mM; and T3, control. The variables evaluated were rooted plantlets, plant height, root length, number of leaves, stem diameter, leaf dry weight, stem dry weight, root dry weight, leaf area, shoot biomass, total biomass, as well as development and growth indexes. Four samplings were conducted in each experiment. The results show that the most appropriate months for propagating stevia cuttings are February, March, April, May, and July, when 96% to 99% of the cuttings rooted. The hormones had the best results related to production of root development. The control was outstanding only in variables related to production of shoot biomass and not to root development. It is concluded that stevia can be propagated vegetatively using cuttings treated with IBA 7.4 mM or ANA 6.4 mM + IBA 0.3 mM, preferable in the period from February to July, with the exception of June.

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“…4). According to Yucesan et al (2016), IAA was more effective for root formation, producing 7.6 roots per shoot with 100% rooting frequency. Similar result was also obtained from 1.0 mgL -1 NAA (RockOkuyucu, 2016).…”

Section: Root Inductionmentioning

confidence: 99%

Mass Production of Stevia rebaudiana (Bertoni) Bertoni for Commercial Cultivation

Fonseka¹,

Wickramaarachchi²,

Aluthgamage³

2018

Int. J. Curr. Res. Biosci. Plant Biol.

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Stevia rebaudiana is a medicinally important herb belongs to Asteraceae family. It is highly important in anti-diabetic treatments due to sweet taste with zero calorific value. Stevia is a natural alternative source to traditional sugar (sucrose) obtained from sugarcane and sugar beet. The aim of this research was to develop a protocol for mass production of this important medicinal herb for commercialization. The experiments were carried out to develop an in vitro culture technique for the mass propagation of Stevia rebaudiana using shoot tips and nodal segments in a Completely Randomized Design (CRD). Explants were collected from 10 weeks old mother plants maintained in shade house conditions. Surface sterilized explants were cultured on MS (Murashiege and Skoog) medium for culture establishment. Established shoot tips and nodal segment explants were transferred to MS medium containing different concentrations and combinations of BAP, Kinetin and NAA. The highest plantlet proliferation was observed in the MS medium supplemented with 0.2 mg/L NAA, 0.5 mg/L BAP and 1 mg/L Kinetin after 6 th week of ex-plant establishment. Rooting was observed in the MS medium supplemented with 0.5 mg L -1 of IBA and 0.2 mg L -1 IAA leading to a successful plantlet regeneration with well-developed shoots and roots that are ready for acclimatization. Highest survival percentage (90%) was observed using coco-pellet as a substrate under high humid conditions.

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In vitro and ex vitro propagation of Stevia rebaudiana Bertoni with high Rebaudioside-A content—A commercial scale application

Cited by 26 publications

References 26 publications

Micropropagation of Stevia rebaudiana plants

Micropropagation of Stevia rebaudiana plants

Growth and Development of Stevia Cuttings During Propagation with Hormones in Different Months of the Year

Mass Production of Stevia rebaudiana (Bertoni) Bertoni for Commercial Cultivation

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