In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2

Abstract

The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography-mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future.

Anticancer activity of secondary metabolite isolated from the rhizospheric fungus Fusarium oxysporum isolate-ABRF1, 2-propenoic acid, pentadecyl ester

“…Rhizospheric fungi were collected from the rhizospheric region of soil from the Achanakmar Biosphere (Bilaspur) in the central mainland of the Indian subcontinent renowned as "Herbal State-Chhattisgarh" by the method of (Radhika and Rodrigues 2010). The fungus was identified and characterized as described previously by (Sahu et al 2020).…”

“…Recent studies showed several bioactive compounds, including antifungal and antibacterial agents, have been isolated from fungi (Sahu et al 2020). The promotion of plant growth is the significant contribution of fungal symbiosis.…”

Study on Antioxidant, Antidiabetic and Antibacterial Activity of Rhizospheric Fungi from Achanakmar Biosphere Reserve, Bilaspur

“…The column purified fractions containing secondary metabolites from YESB extract were also tested for antimicrobial activity based on inhibition of growth of B. Cerculans , B. Subtilis , E. coli , S. Aureus and Ralstonia eutropha (MTCC-2487), (Sahu et al, 2020) at a maximum concentration of 100µg/mL for qualitative assay (Fig. 10 and Table 2).…”