In vitro and in vivo susceptibility of the Lyme disease spirochete, Borrelia burgdorferi, to four antimicrobial agents

Johnson, Russell C.; Kodner, Carrie; Russell, Marie

doi:10.1128/aac.31.2.164

Cited by 136 publications

(99 citation statements)

References 18 publications

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“…Cognizant of these results and that of a treatment study of Brucella infection in mice (12), Bockenstedt et al increased the dose of doxycycline to 50 mg/kg twice daily (8). The MIC of doxycycline for the N40 strain of B. burgdorferi used by Bockenstedt et al has not been reported, but for other strains of B. burgdorferi, MICs of doxycycline are typically 4-to Ͼ10-fold higher than the 0.06-g/ml MIC of doxycycline for the strain of Brucella melitensis in the study relied upon by Bockenstedt et al (19,23). Furthermore, 100% of the mice were cured of the Brucella infection with a 45-day course of doxycycline (50 mg/kg twice daily), whereas a 21-day course of therapy was less effective and a 30-day course of treatment was not attempted (12).…”

Section: Study By Hodzic Et Almentioning

confidence: 99%

“…Studies of the effects of antibiotic therapy in animals infected with B. burgdorferi have been conducted most often with mice (8,18,25,30,31,35,38,53,54) but also with hamsters (21,22), gerbils (15,32,42), dogs (48,49), and nonhuman primates (39). In most of the studies the animals were immunocompetent, but in several the animals were immunocompromised (25,37,48).…”

Section: Animal Studies: General Remarksmentioning

confidence: 99%

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Antibiotic Treatment of Animals Infected withBorrelia burgdorferi

Wormser

Schwartz

2009

Clin Microbiol Rev

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SUMMARY Despite resolution of the objective manifestations of Lyme disease after antibiotic treatment, a minority of patients have fatigue, musculoskeletal pain, and/or difficulties with concentration or short-term memory of uncertain etiology; these are called post-Lyme disease symptoms or, in more severe cases, post-Lyme disease syndrome or “chronic Lyme disease.” Several recent studies in which Borrelia burgdorferi-infected animals were treated with antibiotic therapy have demonstrated the presence of PCR positivity for B. burgdorferi DNA in the absence of culture positivity. In mice that were treated with antibiotic therapy, residual spirochetes could be taken up by ticks during a blood meal and could be transmitted to SCID mice. These spirochetes are attenuated; their presence is not associated with either inflammation or disease. In this review the methodology and findings of these studies are critically analyzed, and the significance of the results with regard to human Lyme disease is evaluated, with special emphasis on whether these studies provide useful insights into post-Lyme disease syndrome. A serious methodological concern is the failure to consider the pharmacokinetic-pharmacodynamic properties of the antibiotic in choosing the dosage regimen used. We conclude that there is no scientific evidence to support the hypothesis that such spirochetes, should they exist in humans, are the cause of post-Lyme disease syndrome.

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Section: Study By Hodzic Et Almentioning

confidence: 99%

Section: Animal Studies: General Remarksmentioning

confidence: 99%

Antibiotic Treatment of Animals Infected withBorrelia burgdorferi

Wormser

Schwartz

2009

Clin Microbiol Rev

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“…The microaerophilic character of the borrelia is indicated by its preference for the bottom portion of the culture medium during initial growth (11,92). Addition of low concentrations (0.1 to 0.2%) of agarose to further thicken the medium improves the recovery of B. burgdorferi from animal fluids and tissues (4,6,94,95). In some cases reducing agents, such as L-cysteine, dithiothreitol, and superoxide dismutase, have been added as supplements.…”

Section: Differential Diagnosismentioning

confidence: 99%

“…In one published study, hamsters were used to assess the efficacy of different antimicrobial agents on B. burgdorferi (94). The (29,135).…”

Section: Differential Diagnosismentioning

confidence: 99%

Laboratory aspects of Lyme borreliosis

Barbour

1988

Clin Microbiol Rev

130

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Lyme borreliosis (Lyme disease), a common tick-borne disorder of people and domestic animals in North America and Europe, is caused by the spirochete Borrelia burgdorferi. Following the discovery and initial propagation of this agent in 1981 came revelations that other tick-associated infectious disorders are but different forms of Lyme borreliosis. A challenge for the clinician and microbiology laboratory is confirmation that a skin rash, a chronic meningitis, an episode of myocarditis, or an arthritic joint is the consequence of B. burgdorferi infection. The diagnosis of Lyme borreliosis may be established by (i) directly observing the spirochete in host fluid or tissue, (ii) recovering the etiologic spirochete from the patient in culture medium or indirectly through inoculation of laboratory animals, or (iii) carrying out serologic tests with the patient's serum or cerebrospinal fluid. The last method, while lacking in discriminatory power, is the most efficacious diagnostic assay for most laboratories at present.

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“…Lyme disease is a multistage disease which has been divided into 3 stages, defined by different clinical features and duration after infection (11,27,28). Since Lyme disease has been a serious social problem in North America and Europe, susceptibilities of Borrelia related to Lyme disease against various antibiotics have been examined for development of successful treatment (1,5,6,9,10,14,15,(25)(26)(27).…”

mentioning

confidence: 99%

In Vitro Antibiotic Susceptibilities of Borrelia Isolates from Erythema Migrans Lesion of Lyme Disease Patients in Japan

Masuzawa

Yamada

Kawabata

et al. 1994

Microbiology and Immunology

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Antibiotic susceptibilities of twelve borrelial isolates from skin of patients with erythema migrans (EM) and ticks (Ixodes persulcatus and I. ovatus) in Japan were examined by in vitro microdilution MIC method and macrodilution MBC method. Nine EM isolates and 3 tick isolates were susceptible to amoxicillin, erythromycin, and minocycline. MICs for Japanese isolates were 0.038–0.30 μg/ml, < 0.012 μg/ml, and < 0.012–0.05 μg/ml, respectively. MBCs were as follows: 0.038–0.88 μg/ml, < 0.012–0.10 μg/ml, and <0.025–0.78 μg/ml, respectively. These antibiotics could be recommended for treatment of patients in early stage of Lyme disease in Japan.

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In vitro and in vivo susceptibility of the Lyme disease spirochete, Borrelia burgdorferi, to four antimicrobial agents

Cited by 136 publications

References 18 publications

Antibiotic Treatment of Animals Infected withBorrelia burgdorferi

Antibiotic Treatment of Animals Infected withBorrelia burgdorferi

Laboratory aspects of Lyme borreliosis

In Vitro Antibiotic Susceptibilities of Borrelia Isolates from Erythema Migrans Lesion of Lyme Disease Patients in Japan

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