In vitro and in vivo anti-metastatic effect of the alkaliod matrine from Sophora flavecens on hepatocellular carcinoma and its mechanisms

Dai, Meiqin; Chen, Nana; Li, Jinzhou; Tan, Lizhuan; Li, Xiaojuan; Wen, Jiayong; Lei, Lin-sheng; Guo, Dan

doi:10.1016/j.phymed.2021.153580

Cited by 20 publications

(9 citation statements)

References 28 publications

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“…Huang et al [ 16 ] found that hsa-miR-199a-5p expression was lower in HCC tissues than in nearby non-tumor tissues. Recently, hsa-miR-199a-5p mimics achieved less HCC cell line survival or colony formation via decreasing the expression of hypoxia-induced factor-1 (HIF-1) [ 17 ]. Nevertheless, the effect of blood hsa-miR-199a-5p was similar to that of the now-mentioned miRs (hsa-miR-21-5p, hsa-miR-155-5p, and hsa-miR-192-5p) together as a unit/panel; HCC must be explored in Egyptian patients with cirrhosis associated with CHCV infection.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on Top of Liver Cirrhosis in HCV-Infected Patients

Eldosoky

Hammad

Elmadbouly

et al. 2023

IJMS

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Early hepatocellular carcinoma (HCC) diagnosis is challenging. Moreover, for patients with alpha-fetoprotein (AFP)-negative HCC, this challenge is augmented. MicroRNAs (miRs) profiles may serve as potential HCC molecular markers. We aimed to assess plasma homo sapiens—(hsa)-miR-21-5p, hsa-miR-155-5p, hsa-miR-192-5p, and hsa-miR-199a-5p—expression levels as a panel of biomarkers for HCC in chronic hepatitis C virus (CHCV) patients with liver cirrhosis (LC), especially AFP-negative HCC cases, as a step toward non-protein coding (nc) RNA precision medicine. Subjects and methods: 79 patients enrolled with CHCV infection with LC, subclassified into an LC group without HCC (n = 40) and LC with HCC (n = 39). Real-time quantitative PCR was used to measure plasma hsa-miR-21-5p, hsa-miR-155-5p, hsa-miR-192-5p, and hsa-miR-199a-5p. Results: Plasma hsa-miR-21-5p and hsa-miR-155-5p demonstrated significant upregulation, while hsa-miR-199a-5p demonstrated significant downregulation in the HCC group (n = 39) when compared to the LC group (n = 40). hsa-miR-21-5p expression was positively correlated with serum AFP, insulin, and insulin resistance (r = 0.5, p < 0.001, r = 0.334, p = 0.01, and r = 0.303, p = 0.02, respectively). According to the ROC curves, for differentiating HCC from LC, combining AFP with each of hsa-miR-21-5p, hsa-miR-155-5p, and miR199a-5p improved the diagnostic sensitivity to 87%, 82%, and 84%, respectively, vs. 69% for AFP alone, with acceptable specificities of 77.5%, 77.5%, and 80%, respectively, and AUC = 0.89, 0.85, and 0.90, respectively vs. 0.85 for AFP alone. hsa-miR-21-5p/hsa-miR-199a-5p and hsa-miR-155-5p/hsa-miR-199a-5p ratios discriminated HCC from LC at AUC = 0.76 and 0.71, respectively, with sensitivities = 94% and 92% and specificities = 48% and 53%, respectively. Upregulation of plasma hsa-miR-21-5p was considered as an independent risk factor for HCC development [OR = 1.198(1.063–1.329), p = 0.002]. Conclusions: Combining each of hsa-miR-21-5p, hsa-miR-155-5p, and hsa-miR-199a-5p with AFP made it possible to identify HCC development in the LC patients’ cohort with higher sensitivity than using AFP alone. hsa-miR-21-5p/hsa-miR-199a-5p and hsa-miR-155-5p/hsa-miR-199a-5p ratios are potential HCC molecular markers for AFP-negative HCC patients. hsa-miR-21-5p was linked, clinically and via in silico proof, to insulin metabolism, inflammation, dyslipidemia, and tumorigenesis in the HCC patients’ group as well as for an upregulated independent risk factor for the emergence of HCC from LC in the CHCV patients.

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Section: Introductionmentioning

confidence: 99%

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on Top of Liver Cirrhosis in HCV-Infected Patients

Eldosoky

Hammad

Elmadbouly

et al. 2023

IJMS

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“…Matrine exists in dried roots of Sophora flavescent and induces HepG2 cell apoptosis through increasing Bax/Bcl-2 ratio and inhibiting the ERK1/2 signaling pathway (18). Dai et al reported that matrine inhibits BEL-7402 cell and SMMC-7721 cell invasion and metastasis by upregulating the expression of microRNA-199a-5p (miR-199a-5p) and reduces the levels of hypoxia-inducible factor-1a (HIF-1a) (19). Moreover, matrine also inhibits liver cancer migration and induces apoptosis through increasing the expression of microRNA-345-5p (miR-345-5p) and reduces the levels of circular RNA_0027345 (circ_0027345) and homeobox-containingD3 (HOXD3) (20).…”

Section: Plant-derived Natural Products In the Treatment Of Liver Can...mentioning

confidence: 99%

Plant-derived natural products and combination therapy in liver cancer

Li¹,

Li²

2023

Front. Oncol.

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Liver cancer is one of the malignant cancers globally and seriously endangers human health because of its high morbidity and mortality. Plant-derived natural products have been evaluated as potential anticancer drugs due to low side effects and high anti-tumor efficacy. However, plant-derived natural products also have defects of poor solubility and cumbersome extraction process. In recent years, a growing numbers of plant derived natural products have been used in combination therapy of liver cancer with conventional chemotherapeutic agents, which has improved clinical efficacy through multiple mechanisms, including inhibition of tumor growth, induction of apoptosis, suppression of angiogenesis, enhancement of immunity, reversal of multiple drug resistance and reduction of side effects. The therapeutic effects and mechanisms of plant-derived natural products and combination therapy on liver cancer are reviewed to provide references for developing anti-liver-cancer strategies with high efficacy and low side effects.

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“…Extracted from the dried root of Sophora flavescent Ait. and Euchresta japonica, matrine has antiarrhythmia [ 85 ], antibacterial [ 56 ], antipulmonary [ 86 ], hepatic fibrosis [ 87 ], and antitumor [ 88 ] effects. As a broad-spectrum antiarrhythmia drug, matrine can prolong action potential duration and effective refractory period, slow down heart rate, improve myocardial contractility, and inhibit ectopic rhythm and atrioventricular reentry agitation by inhibiting potassium, sodium, and calcium channels [ 89 ].…”

Section: Natural Products With Bioactivity In Afmentioning

confidence: 99%

Research Progress on Natural Products’ Therapeutic Effects on Atrial Fibrillation by Regulating Ion Channels

Ding

et al. 2022

Cardiovascular Therapeutics

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Antiarrhythmic drugs (AADs) have a therapeutic effect on atrial fibrillation (AF) by regulating the function of ion channels. However, several adverse effects and high recurrence rates after drug withdrawal seriously affect patients’ medication compliance and clinical prognosis. Thus, safer and more effective drugs are urgently needed. Active components extracted from natural products are potential choices for AF therapy. Natural products like Panax notoginseng (Burk.) F.H. Chen, Sophora flavescens Ait., Stephania tetrandra S. Moore., Pueraria lobata (Willd.) Ohwi var. thomsonii (Benth.) Vaniot der Maesen., and Coptis chinensis Franch. have a long history in the treatment of arrhythmia, myocardial infarction, stroke, and heart failure in China. Based on the classification of chemical structures, this article discussed the natural product components’ therapeutic effects on atrial fibrillation by regulating ion channels, connexins, and expression of related genes, in order to provide a reference for development of therapeutic drugs for atrial fibrillation.

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In vitro and in vivo anti-metastatic effect of the alkaliod matrine from Sophora flavecens on hepatocellular carcinoma and its mechanisms

Cited by 20 publications

References 28 publications

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on Top of Liver Cirrhosis in HCV-Infected Patients

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on Top of Liver Cirrhosis in HCV-Infected Patients

Plant-derived natural products and combination therapy in liver cancer

Research Progress on Natural Products’ Therapeutic Effects on Atrial Fibrillation by Regulating Ion Channels

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