In vitro anthelmintic activity and phytochemical characterization of Corallocarpus epigaeus (Rottler) Hook. f. tuber from ethyl acetate extracts

Ishnava, Kalpesh B.; Konar, Priya S.

doi:10.1186/s42269-020-00286-z

Cited by 17 publications

(13 citation statements)

References 11 publications

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“…Worms were cleaned with tap water for the removal of the adhering dirt. The procedure for the anthelmintic assay was followed as per the reported procedure 25 . Indian earthworm viz.…”

Section: Fig 1: Umbelliferone Structure Materials and Methodsmentioning

confidence: 99%

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2021

IJPSR

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The present study was aimed to evaluate the in-vitro anthelmintic potential of isolated phytoconstituent umbelliferone against Pheretima Posthuma (Indian earthworm). Methods: Three different concentrations (10, 20, 30 mg/ml in Dimethyl sulfoxide (DMSO) of umbelliferone were prepared, and six worms of similar type were placed in it. Observations were made for the time taken for paralysis and death of the individual worm. Meantime required for the paralysis (P) in min was noted when no movement of any sort could be observed, except when the worm was shaken vigorously; death time (D) in min was recorded after confirmation with lack of movement when shaken vigorously/ dipped in warm water (50 °C). Piperazine citrate (10 mg/ml) was used as a reference standard. Results: Umbelliferone demonstrated paralysis as well as the death of worms, especially at a higher concentration of 30 mg/ml in a shorter time as compared to reference drug Piperazine citrate. Conclusion: In the present study, Umbelliferone was tested for its anthelmintic activity against Pheretima posthuma. Various concentrations were used in the bioassay, which involved paralysis and death time of the worms. The phytoconstituent showed significant anthelmintic activity.

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“…Worms were cleaned with tap water for the removal of the adhering dirt. The procedure for the anthelmintic assay was followed as per the reported procedure 25 . Indian earthworm viz.…”

Section: Fig 1: Umbelliferone Structure Materials and Methodsmentioning

confidence: 99%

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2021

IJPSR

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“…Anthelmintic potential of the stem-bark extracts of V. camporum was examined using earthworms (i.e., Lumbricus terrestris), due to their physiological and anatomical resemblance with that of intestinal roundworm parasites of human beings as well as their ease of availability [41,66]. Methanol and hexane extracts and albendazole (standard drug) displayed concentration-dependent anthelmintic activity at test concentrations of 0.5 to 8.0 mg/mL as shown in Figures 2 and 3.…”

Section: Anthelminthic Activitymentioning

confidence: 99%

Anthelminthic, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem-Bark

Akoto

Acheampong

Boakye

et al. 2021

Journal of Chemistry

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Vernonia camporum is used ethnomedicinally to treat diseases such as malaria, fever, hypertension, mouth sores, pains, inflammation, and skin rashes in Africa. This study aims at investigating the biological activities (anthelminthic, anti-inflammatory, antioxidant, and antimicrobial) and separating some of the biologically active components in the methanol crude extract of the stem-bark of Vernonia camporum. Phytochemical screening, anthelminthic (Lumbricus terrestris (earthworm)), anti-inflammatory (egg albumin denaturation), antioxidant (DPPH and H2O2 scavenging and total antioxidant capacity (TAC)), and antimicrobial (agar and broth dilution method) assays were performed on the extracts. Column chromatography and FTIR spectroscopic analysis were employed to separate and analyze the least polar constituents of the methanol extract. The preliminary phytochemical analyses revealed the presence of secondary metabolites such as alkaloids, phenols, tannins, saponins, terpenoids, flavonoids, steroids, phytosterols, proteins and amino acids, phlobatannins, glycosides, cardiac glycosides, coumarins, and anthocyanins. The extracts (methanol and hexane) showed a higher concentration-dependent anthelminthic activity. The extracts exhibited high concentration-dependent anti-inflammatory activities with IC50 values of 35.83 ± 3.984 and 53.91 ± 5.8413 µg/mL, respectively. The IC50 values for methanol and hexane extracts in the DPPH assay were 17.70 ± 3.430 and 29.54 ± 1.5437 µg/mL, and those of the H2O2 assay were 243.20 ± 0.1528 and 352.20 ± 12.64 µg/mL, respectively. TAC results for methanol and hexane extracts were recorded as 31.592 ± 1.682 and 30.232 ± 0.445 gAAE/100 g, respectively. The extracts were observed to exhibit antimicrobial activity against test organisms with MICs ranging from 0.1953 to 25.00 mg/mL. Chromatographic separations gave four fractions. FTIR analysis indicated the presence of various functional groups in purified fractions of the methanol extract that confirms the presence of the phytochemicals identified in the screening test. The results indicate that both extracts of V. camporum possess anthelminthic, anti-inflammatory, antioxidant, and antimicrobial activities supporting the traditional usage of this plant.

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“…In traditional medicine, the root tuber is used for leprosy, goiter, anemia, abdominal pain, skin disorders, and as an antidote for poisoning. [29]…”

Section: Corallocarpus Epigaeus (Rottler) (Redfruit Creeper)mentioning

confidence: 99%

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Varnakulendran¹

2021

IJGP

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Introduction:The crude drugs are untreated substances derived from organic or inorganic sources such as plants, metal, mineral, and animal or their parts. This study is focused on plants sources obtained from underneath the ground chiefly roots and underground stems. Siddha system of medicine is an ancient system of indigenous medicine practiced in several parts of the world. Materials and Methods: The information is gathered through scientific research articles from the reputed website such as PubMed, Elsevier, and Web of Science. Results and Discussion: The roots are specially modified into underground stems for storage of starch and water. Rhizomes are also a type of swollen stem that grows underground, such as ginger and turmeric. Tubers with their lumpy, swollen, and starchy underground stem, potatoes are a very familiar example of tubers. Onions are a classic example of a bulb when sliced in half, they clearly display the concentric rings that are the telltale sign of a bulb. Elephant yam is a type of corm, swollen stem base that is modified into a mass of storage tissue. A corm does not have visible storage rings when cut into half. This distinguishes it from a true bulb. This review highlights the medicinal plant's roots and underground stems used in the traditional medicines. Electronic databases were utilized to analyze the ethnopharmacological effects of the roots and underground stems of the herbs. Conclusion: Plenty of phytochemical studies done in different analytical ways which revealed the potency of the roots and underground stems of these herbs. Moreover, this information has prompted to compile the list of roots and underground stems used in the traditional system of medicine to cure many health manifestations.

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In vitro anthelmintic activity and phytochemical characterization of Corallocarpus epigaeus (Rottler) Hook. f. tuber from ethyl acetate extracts

Cited by 17 publications

References 11 publications

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Anthelminthic, Anti-Inflammatory, Antioxidant, and Antimicrobial Activities and FTIR Analyses of Vernonia camporum Stem-Bark

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