In vitro anthelmintic effects of Bridelia ferruginea, Combretum glutinosum, and Mitragyna inermis leaf extracts on Haemonchus contortus, an abomasal nematode of small ruminants

Alowanou, Géorcelin Goué; Olounladé, Pascal Abiodoun; Akouedegni, Guénolé Coovi; Faihun, A. M. L.; Koudandé, Delphin O.; Hounzangbé-Adoté, Sylvie

doi:10.1007/s00436-019-06262-5

Cited by 22 publications

(27 citation statements)

References 20 publications

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“…Numerous other applied studies have demonstrated the effect of tannin in the control of GIN at all stages of development, from the inhibition of larval exsheathment [11,32,[34][35][36], inhibition of larval migration of L3s [12,37,38], inhibition of egg hatch [30], inhibition of adult worm motility [11,30] or structural lesions on larvae or adult worms [39,40]. In this anthelmintic response, the hypothesis of a direct mode of action of the tannins has often been evoked but also the improvement of the host immune response [41].…”

Section: Discussionmentioning

confidence: 99%

“…Extraction procedure and the yield of extracts were previously described [30]. Briefly, 50 grams (50 g) of powder from the leaves of each plant (B. ferruginea and M. inermis) were weighed and mixed in 500 mL of acetone and distilled water in a 70:30 ratio of distilled water-acetone.…”

Section: Extraction Proceduresmentioning

confidence: 99%

“…Third-stage infective larvae (L3s) were obtained following procedure described previously [30]. Briefly, faeces from sheep previously artificially infested with pure strains of H. contortus were collected and left in culture at room temperature for 10 days.…”

Section: Obtaining L3s Of H Contortusmentioning

confidence: 99%

“…Scientific work has been carried out on B. ferruginea and M. inermis on phytochemical screening [19,[26][27][28][29][30] and the evaluation of the AHs properties attributed to them by the traditional pharmacopeia [10,31]. However, the mechanism of action of these plants on the GINs of small ruminants and the biological activity of secondary metabolites are not yet well known both in vivo and in vitro.…”

Section: Introductionmentioning

confidence: 99%

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In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus

Tchetan

Azando

Olounladé

et al. 2020

International Journal of Veterinary Science and Medicine

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Bridelia ferruginea (Euphorbiaceae) and Mitragyna inermis (Rubiaceae) are two plants of the beninese pharmacopeia used in vivo for the control of gastrointestinal nematodes (GINs) in small ruminants. The objective of the present study is to explore the mechanism of bioactive compounds involved in the action of these two plants on the third-stage infective larvae (L3s) of Haemonchus contortus. Thus, sheathed L3s of H. contortus were incubated with acetone extracts of B. ferruginea and M. inermis at concentrations of 0, 150, 300, 600 and 1200 µg/mL for 3 h at 25°C. The L3s were then washed and artificially submitted to exsheathment in the presence of sodium hypochlorite solution. The role of tannins was verified by adding a tannin inhibitor, polyvinyl polypyrrolidone (PVPP), to the acetone extracts of these two plants for 2 h at 25°C. Acetone extracts from B. ferruginea and M. inermis inhibited the exsheathment of H. contortus larvae (p < 0.001) and this inhibitory effect was dose-dependent for M. inermis at the concentrations tested. Treatment of B. ferruginea and M. inermis extracts with PVPP was associated with a partial restoration of the exsheathment kinetics of H. contortus larvae (p < 0.001), confirming the predominant role of tannins but also the residual role of other secondary metabolites. These in vitro results suggest that these plants are endowed with anthelmintic (AHs) properties and therefore likely to be used as alternatives to synthetic molecules.

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Section: Discussionmentioning

confidence: 99%

Section: Extraction Proceduresmentioning

confidence: 99%

Section: Obtaining L3s Of H Contortusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus

Tchetan

Azando

Olounladé

et al. 2020

International Journal of Veterinary Science and Medicine

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“…Indeed, the leaves of this plant are widely consumed by ruminants as a drug plant. They are also combined with dietary supplements by breeders to help small ruminants resist helminthiasis (Alowanou et al, 2019). Fresh leaves can be used as a borehole for infested animals, they are directly used to prepare the sorted extract that was used for the various tests as part of this work.…”

Section: Inhibition Of the Motility Of Adult Worms:-mentioning

confidence: 99%

Phytochemical Screening and in Vitro Effect of Watery Triturated Extract of Mitragyna Inermis (Willd.) Kuntz (Rubiaceae) on Haemonchus Contortus

Mahougnan¹,

Yayi²,

Sakirigui³

et al. 2019

IJAR

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Environmental impact of phytobiotic additives on greenhouse gas emission reduction, rumen fermentation manipulation, and performance in ruminants: an updated review

Ahmed,

Elwakeel,

El-Zarkouny

et al. 2024

Environ Sci Pollut Res

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Ruminal fermentation is a natural process involving beneficial microorganisms that contribute to the production of valuable products and efficient nutrient conversion. However, it also leads to the emission of greenhouse gases, which have detrimental effects on the environment and animal productivity. Phytobiotic additives have emerged as a potential solution to these challenges, offering benefits in terms of rumen fermentation modulation, pollution reduction, and improved animal health and performance. This updated review aims to provide a comprehensive understanding of the specific benefits of phytobiotic additives in ruminant nutrition by summarizing existing studies. Phytobiotic additives, rich in secondary metabolites such as tannins, saponins, alkaloids, and essential oils, have demonstrated biological properties that positively influence rumen fermentation and enhance animal health and productivity. These additives contribute to environmental protection by effectively reducing nitrogen excretion and methane emissions from ruminants. Furthermore, they inhibit microbial respiration and nitrification in soil, thereby minimizing nitrous oxide emissions. In addition to their environmental impact, phytobiotic additives improve rumen manipulation, leading to increased ruminant productivity and improved quality of animal products. Their multifaceted properties, including anthelmintic, antioxidant, antimicrobial, and immunomodulatory effects, further contribute to the health and well-being of both animals and humans. The potential synergistic effects of combining phytobiotic additives with probiotics are also explored, highlighting the need for further research in this area. In conclusion, phytobiotic additives show great promise as sustainable and effective solutions for improving ruminant nutrition and addressing environmental challenges.

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In vitro anthelmintic effects of Bridelia ferruginea, Combretum glutinosum, and Mitragyna inermis leaf extracts on Haemonchus contortus, an abomasal nematode of small ruminants

Cited by 22 publications

References 20 publications

In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus

In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus

Phytochemical Screening and in Vitro Effect of Watery Triturated Extract of Mitragyna Inermis (Willd.) Kuntz (Rubiaceae) on Haemonchus Contortus

Environmental impact of phytobiotic additives on greenhouse gas emission reduction, rumen fermentation manipulation, and performance in ruminants: an updated review

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