In Vitro Anti-Helicobacter pylori Effects of Medicinal Mushroom Extracts, with Special Emphasis on the Lion's Mane Mushroom, Hericium erinaceus (Higher Basidiomycetes)

Abstract

Although the medicinal mushroom Hericium erinaceus is used extensively in traditional Chinese medicine to treat chronic superficial gastritis, the underlining pharmaceutical mechanism is yet to be fully understood. In this study, minimum inhibitory concentration (MIC) values of extracts prepared from the fruiting bodies of 14 mushroom species (H. erinaceus, Ganoderma lucidum, Cordyceps militaris, Pleurotus eryngii, P. ostreatus, Agrocybe aegerita, Lentinus edodes, Agaricus brasiliensis, A. bisporus, Coprinus comatus, Grifola frondosa, Phellinus igniarius, Flammulina velutipes, and Hypsizygus marmoreus) were determined against Helicobacter pylori using laboratory strains of ATCC 43504 and SS1 as well as 9 clinical isolates via an in vitro microplate agar diffusion assay. Ethanol extracts (EEs) of 12 mushrooms inhibited the growth of H. pylori in vitro, with MIC values <3 mg/mL. EEs of H. erinaceus and G. lucidum also inhibited Staphylococcus aureus (MIC 7360;10 mg/mL) but had no effect on the growth of two Escherichia coli test strains (MIC >10 mg/mL). MIC values of ethyl acetate fractions (EAFs) of H. erinaceus against 9 clinical isolates of H. pylori ranged between 62.5 and 250 µg/mL. The bacteriostatic activity of EAFs was found to be concentration-dependant, and the half maximal inhibitory concentration and minimum bactericidal concentration values for H. pylori ATCC 43504 were 73.0 and 200 µg/mL, respectively. The direct inhibitory effect of EEs and EAFs of H. erinaceus against H. pylori could be another pharmaceutical mechanism of medicinal mushrooms-besides the immunomodulating effect of polysaccharides, suggested previously-in the treatment of H. pylori-associated gastrointestinal disorders. Further research to identify the active component(s) is currently undertaking in our laboratory.

Chemistry, Nutrition, and Health-Promoting Properties of Hericium erinaceus (Lion’s Mane) Mushroom Fruiting Bodies and Mycelia and Their Bioactive Compounds

“…H. pylori is a human gastrointestinal pathogen that may contribute to the cause of adverse effects, possibly including ulcers. Shang et al 85 determined the minimum inhibitory concentration (MIC) values of extracts prepared from the fruiting bodies of 14 mushroom species including H. erinaceus against H. pylori clinical strains using a microplate agar diffusion assay. MIC values of ethyl acetate fractions of H. erinaceus against nine clinical isolates of H. pylori ranged between 62.5 and 250 μg/mL.…”

The preventive effect of cellulose from Pleurotus eryngii on fatty liver of hyperlipidemic rats

“…Additionally, Pleurotus eryngii could inhibit the growth of helicobacter pylori, Escherichia coli (E. coli), staphylococcus aureus and listeria strains, prevent from virus infection including H1N1, HSV-2 and so on [9][10][11]. [12].…”

Bioactivities and Health Benefits of Mushrooms Mainly from China

“…Agaricus bisporus Neurospora sitophila, phytopathogenic fungi [83] Lenzites betulina Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Fusarium graminearum, Gibberella zeae and Cercosporella albo-maculans [84] Agrocybe cylindracea Several fungal species [85] Tricholoma giganteum Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola [86] Hericium erinaceus Helicobacter pylori [87] Clitocybe sinopica Agrobacterium rhizogenes, A. tumefaciens, A. vitis, Xanthomonas oryzae and X. malvacearum [78] Pleurotus ostreatus Fusaerium oxysporum, Mycosphaerella arachidicola and Physalospora piricola [88] …”