In Vitro Anti Sickling Activity of HyphaeneThebaica Fruits Extract on Sickle Cell

Abstract

Background: Traditional medicine had been used alongside synthetic pharmaceutical products to enhance health management. Due to the high mortality rate of sickle cell patients, previous studies have been indicated that some medicinal plants have shown an anti-sickling activity, which indicates a new therapeutic way to manage people who are affected by these disorders. The current study aimed to assess in vitro-anti-sickling activity of Hyphaene thebaica (H thebaica) (Doum) fruit. Materials and methods: Blood samples used in the evaluation of the anti-sickling activity of Hyphaene Thebaica Fruits extract in this study was taken from patients known to had Sickle cell disease (HB -SS ) attending the Sickle Cell Clinic in Khartoum state. Emmel test was used to assess anti-sickling activity of this plant. Result: A significant increase in the percentage of unsickled Red blood cells with p-value 0.05 >was observed after incubation of RBCs with 2% sodium metabisulfite in the presence of three different concentration (1000, 500 and 250) μg/ml of Hyphened thebaica for two type of extraction (aqueous and methanol) extract. Conclusion: This study approved that H.thebaica (Doum ) fruit extract had a strong anti- sickling activity ; it could be used for management of sickle cell disease.