In vitro antifungal evaluation and studies on mode of action of eight selected species from the Argentine flora

Zacchino, Susana; Santecchia, C.; López, Silvia N.; Gattuso, Susana; Muñoz, Juan de Dios; Cruañes, A.; Vivot, Eduardo P.; Cruañes, M. del C.; Salinas, Adriana; Ruiz, R.E. de; Ruiz, S.

doi:10.1016/s0944-7113(98)80022-6

Cited by 41 publications

(28 citation statements)

References 16 publications

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“…The dichloromethane extract of its aerial parts showed a strong antifungal activity in our screening procedure using the bioautographic assay with spores of C. sphaerospermum. This result is in agreement with the findings published by Zacchino et al (1998) showing that the dichloromethane extracts of the aerial parts of P. punctatum was active against a panel of yeasts, filamentous fungi and dermatophytes. Interestingly, Rahalison et al (1993) reported that the extract from the roots of P. punctatum collected in Panama was inactive when tested in a similar assay using Cladosporium curcumerium and Candida albicans.…”

Section: Resultssupporting

confidence: 93%

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Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum

Alves

Kloos

et al. 2001

Mem. Inst. Oswaldo Cruz

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Polygonum punctatum (Polygonaceae) is an herb known in some regions of Brazil as "erva-debicho" and is used Several plant species of the genus Polygonum (Polygonaceae) are used in the folk medicine at different parts of the world to treat many diseases including skin infections, dysentery, snake-bite, hemorrhoids, insomnia and heart diseases and is also used for liver protection (WHO 1989a(WHO ,b, 1998a. According to the NAPRALERT database, in Latin America and North America Polygonum punctatum Elliot is used in some areas to treat a diversity of ailments including hemorrhoids, diarrhoea, colds and influenza. In Brazil, P. punctatum (syn. Persicaria punctata and P. acre) is popularly known as "erva-de-bicho", "capiçoba", "pimenta-d'água", "pimenta-do-brejo" and "cataia" (Corrêa 1984). It is used to treat hemorrhoids and rheumatism, as an abortive, a diuretic and a hemenagogue (Martins et al. 1995). Previous pharmacological studies with the ethanol/water extract of the entire plant disclosed antihistaminic, anti-inflammatory, antipyretic and hypotensive activities (Oliveira-Simões et al. 1989). Toxicity assays of the methanolic and aqueous extracts in the rat model indicated an LC 50 > 1g/kg (Bhakuni et al. 1969).In our search for bioactive compounds from the Brazilian flora, we screened hundreds of plant extracts, including those from medicinal plants, for fungitoxic activity against Cladosporium sphaerospermum (Alves et al. 2000) using a bioautographic assay based on the protocol described by Homans and Fuchs (1970). The crude dichloromethane extract of aerial parts of P. punctatum was one of the most active. Aiming to isolate and identify the fungitoxic compound(s) we investigated this extract using a bioassay-guided chemical fractionation protocol. MATERIALS AND METHODSPlant collection -The aerial parts of the plant were collected at Governador Valadares, State of Minas Gerais, Brazil. A voucher specimen was deposited in the Department of Botany Herbarium, Universidade Federal de Minas Gerais, under the code BHCB 41475.Extraction -The plant was dried at 45°C in a convection oven and powdered in a knife mill. The powder (85 g) was macerated at room temperature for 24 h in dichloromethane. After filtration, the solvent was removed by rotary evaporation under reduced pressure and at temperatures below 45°C affording 2 g of crude extract.Isolation and identification of pure compound -The crude extract (500 mg) was submitted to centrifugal circular chromatography using hexane/ ethanol/water (10:9:1). Thirty fractions were collected. The fungitoxic fractions 11 to 13 were grouped (31 mg) and chromatographed on a silicagel column eluted with dichloromethane. A white solid (5 mg, 0.23% yield from dry plant) was obtained as the active compound.Bioautographic assay with C. sphaerospermum-The procedure of Homans and Fuchs (1970) was adapted. Briefly, the extract, fractions 832 Fungitoxic Component from P. punctatum Tânia Maria de Almeida Alves et al.and pure compound (100 µg) were spotted on TLC plates using the app...

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Section: Resultssupporting

confidence: 93%

“…Hagendoorn et al (1994) demonstrated that in P. hydropiper, polygodial is present only in the aerial parts of the plant. This fact, together with our findings and those of Zacchino et al (1998) and Rahalison et al (1993), allow us to hypothesize that in P. punctatum too, polygodial is restricted to the aerial parts of the plant.…”

Section: Resultssupporting

confidence: 84%

Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum

Alves

Kloos

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…(Tiliaceae), known in Brazil as "açoita-cavalo", is a tree which grows in Brazil, Argentina and Paraguay. 2,3 The L. divaricata is used in Brazilian folk medicine for different purposes: the leaves are used as diuretic, the stems as antiinflammatory, 4 the bark and aerial parts are used for healing skin wounds, pimples, and for vaginal washes.…”

Section: Introductionmentioning

confidence: 99%

A New tormentic acid derivative from Luehea divaricata Mart. (Tiliaceae)

Tanaka

Vidotti

Silva

2003

J. Braz. Chem. Soc.

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Um extrato metanólico das folhas de Luehea divaricata (Tiliaceae), vulgarmente conhecida no Brasil como "açoita-cavalo", forneceu um novo triterpeno caracterizado como ácido 3β-phidroxibenzoiloxitormentico [ácido 3β-(p-hidroxibenzoiloxi)-2α-hidroxiurs-12-en-28-óico], juntamente com uma mistura contendo o ácido maslínico, um triterpeno conhecido. As estruturas dos compostos foram estabelecidas por métodos espectroscópicos.A methanolic extract from leaves of Luehea divaricata (Tiliaceae), known in Brazil as "açoita-cavalo", yielded two triterpene: a novel characterized as 3β-p-hydroxybenzoyloxytormentic acid [3β-(p-hydroxybenzoyloxy)-2α-hydroxyurs-12-en-28-oic acid] and a mixture containing the maslinic acid. The new compound's structure was established by spectroscopic methods.

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“…The fungistatic activity was evaluated by the agar dilution method using Sabouraud-chloramphenicol agar for yeast, filamentous fungi and dermatophytes, according to reported procedures (Mitscher et al, 1972;Zacchino et al, 1998Zacchino et al, , 1999Feresin et al, 2001 …”

Section: Antifungal Assays Of P Glomeratamentioning

confidence: 99%

Botanical (morphological, micrographic), chemical and pharmacological characteristics of Pfaffia species (Amaranthaceae) native to South Brazil

Gosmann¹,

Gattuso²,

Gattuso³

et al. 2003

Rev. Bras. Cienc. Farm.

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Some parameters for the quality control of P. glomerata and P. paniculata roots using their botanical and chemical characteristics are presented. It was also carried out an in vitro pharmacological screening to evaluate some biological properties of P. glomerata that could be related to its popular use as "tonic". Relating to biological assays, ethanolic extract from P. glomerata roots did not present antiviral, antiproliferative, antifungal or MAO inhibitory activities. The cytotoxicity evaluation of P. glomerata determined that IC 50 is >2,000 μg/mL. The main morphological and micrographic characteristics of P. glomerata and P. paniculata roots are described in this paper in order to aid in their unequivocal identification. UNITERMS:• Pfaffia glomerata

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In vitro antifungal evaluation and studies on mode of action of eight selected species from the Argentine flora

Cited by 41 publications

References 16 publications

Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum

Polygodial, the fungitoxic component from the Brazilian medicinal plant Polygonum punctatum

A New tormentic acid derivative from Luehea divaricata Mart. (Tiliaceae)

Botanical (morphological, micrographic), chemical and pharmacological characteristics of Pfaffia species (Amaranthaceae) native to South Brazil

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