In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 – 2015

Deshmukh, Anand; Hagen, Ferry; Sharabasi, Ola Al; Abraham, Mariamma; Wilson, Godwin; Sanjay, Doiphode; Maslamani, Muna Al; Meis, Jacques F.

doi:10.1186/s12866-015-0458-9

Cited by 38 publications

(34 citation statements)

References 29 publications

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“…Moreover, in another study of 231 isolates of B. melitensis in Qatar, increased MIC of RIF was reported in 48% of isolates. All isolates were sensitive to DOX, STR, GEN, CIP, and SXT (23). Increased MIC of RIF was reported in 70% of 41 Brucella isolates in Malaysia (21).…”

Section: Discussionmentioning

confidence: 97%

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

et al. 2017

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Section: Discussionmentioning

confidence: 97%

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

et al. 2017

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“…Micro-dilution and/or gradient strip (E-test) methods are used to establish minimum inhibitory concentration (MIC) for antimicrobials [45,46]. PCR assays and the subsequent sequencing of genes associated with resistance are used to identify the genetic bases of resistance [47][48][49].…”

Section: Introductionmentioning

confidence: 99%

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

et al. 2019

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Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their antimicrobial resistance. In total, 34-suspected Brucella isolates were recovered from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differentiation and genotyping were confirmed using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR. Antimicrobial susceptibility testing against clinically used antimicrobial agents (chloramphenicol, ciprofloxacin, erythromycin, gentamicin, imipenem, rifampicin, streptomycin, and tetracycline) was performed using E-Test. The antimicrobial resistance-associated genes and mutations in Brucella isolates were confirmed using molecular tools. In total, 29 Brucella isolates (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were identified and typed. The resistance of B. melitensis to ciprofloxacin, erythromycin, imipenem, rifampicin, and streptomycin were 76.2%, 19.0%, 76.2%, 66.7%, and 4.8%, respectively. Whereas, 25.0%, 87.5%, 25.0%, and 37.5% of B. abortus were resistant to ciprofloxacin, erythromycin, imipenem, and rifampicin, respectively. Mutations in the rpoB gene associated with rifampicin resistance were identified in all phenotypically resistant isolates. Mutations in gyrA and gyrB genes associated with ciprofloxacin resistance were identified in four phenotypically resistant isolates of B. melitensis. This is the first study highlighting the antimicrobial resistance in Brucella isolated from different animal species in Egypt. Mutations detected in genes associated with antimicrobial resistance unravel the molecular mechanisms of resistance in Brucella isolates from Egypt. The mutations in the rpoB gene in phenotypically resistant B. abortus isolates in this study were reported for the first time in Egypt.

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“…Brucellosis is transmitted to humans following direct contact with infected animals or indirectly through the consumption of unpasteurized animal products (Deshmukh et al, 2015). The disease causes severe morbidity in humans and results in many medical challenges globally, especially in poor regions (Corbel, 1997; Pappas et al, 2006; Ducrotoy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

Liu

Wang

et al. 2017

Front. Microbiol.

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Brucellosis is a serious public health problem in Ulanqab, which is a region located in the middle of the Inner Mongolia Autonomous Region adjacent to Shanxi and Hebei provinces. The disease is prevalent in both the latter provinces and Ulanqab with the highest prevalence of brucellosis occurring in Inner Mongolia. The MLVA-16 scheme is a genotyping tool for assessing genetic diversity and relationships among isolates. Moreover, this genotyping tool can also be applied to epidemiological trace-back investigations. This study reports the occurrence of at least two B. melitensis biovars (1 and 3) in Ulanqab, encompassing 22 and 94 isolates, respectively. B. melitensis biovar 3 was the predominant biovar in the area examined. Panel 1 (MLVA-8) identified three genotypes (42, 63, and 114), with genotype 42 (n = 101) representing 87% of the tested strains. MLVA-11 identified eight genotypes (116, 111, 297, 291, and 342–345) from 116 of the analyzed isolates. All of these isolates were identified as belonging to the East Mediterranean group. Genotype 116 (n = 94) was the predominant genotype and represented 81% of the isolates. The isolates pertaining to this genotype were distributed throughout most of Ulanqab and neighboring regions. The MLVA-16 scheme showed the presence of 69 genotypes, with 46 genotypes being represented by single isolates. This analysis revealed that Ulanqab brucellosis cases had epidemiologically unrelated and sporadic characteristics. The remaining 23 genotypes were shared (between a total of 70 isolates) with each genotype being represented by two to eight isolates. These data indicate that these cases were epidemiologically related. MLVA genotyping confirmed the occurrence of a multipoint outbreak epidemic and intrafamilial brucellosis. Extensive genotype-sharing events were observed among isolates from different regions of Ulanqab and from other provinces of China. These findings suggest either a lack of control of animal movement between different regions or the circulation of contaminated animal products in the market. Our study is the first comprehensive genotyping and genetic analysis of B. melitensis in Ulanqab. We believe that this study will help to improve the effectiveness of brucellosis control programs.

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In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 – 2015

Cited by 38 publications

References 29 publications

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

Clinical and Diagnostic Aspects of Brucellosis and Antimicrobial Susceptibility of <i>Brucella</i> Isolates in Hamedan, Iran

Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt

MLVA Genotyping Characteristics of Human Brucella melitensis Isolated from Ulanqab of Inner Mongolia, China

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