In vitro Antioxidant Activity and Inhibition of Fe2+ and SNP Lipid Peroxidation of African Mistletoes (Tapinanthus globiferus) from Three Selected Host Plants in Jos Plateau State Nigeria

Abstract

Aim: The aim of this study was to investigate and compare the antioxidative properties of the mistletoe plant obtained from three different host species namely Psidium guajava, Vernonia amygdalina and Moringa olifera lam.
 Study Design: Experimental Design
 Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences and Department of Biochemistry, College of Health Sciences, University of Jos, Nigeria.
 Methodology: Crude methanolic leaf extracts were studied for their antioxidative properties; Iron reducing and Iron-chelating activities, Nitric oxide (NO) radical and 2,2-diphenyl 1-picrylhydrazyl (DPPH) radical scavenging activities and the lipid peroxidation and thiobarbituric acid reaction (TBAR) methods. One way ANOVA was used for the result analysis with P<.05 for significant difference.
 Results: Mistletoes from Psidum guajava (MSPG) had significantly higher reducing property (0.16 – 0.20mg/mL); the chelating property of Mistletoes from Moringa olifera (MSMO) was significantly lower (45.7 – 58.9%); DPPH radical scavenging activity had no significant difference; and Nitric oxide scavenging activity was significantly higher in MSPG (72.1% in 75mg/mL) than the extracts from other hosts. MSPG had significantly higher TBAR inhibition using both FeSO4 (77.8% at 125µg/mL) and Sodium nitroprusside (61.6+1.0% at 125µg/mL) with an IC50 of 30.27µg/mL . Extract of Tapinanthus globiferus leaves from Psidium guajava had more antioxidative activities in the TBARs followed by Tapinanthus globiferus leaf extract from Vernonia amygdalina (MSVA).
 Conclusion: From the study, mistletoes from Psidium guajava had higher antioxidant activity compared to other hosts, which probably justifies its use for treatment of cancer in traditional medicinal practice.

Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices

“…The chelating activities of pomegranate leaves and peels, fig leaves, guava leaves and olive leaves crude juices were determined following the method described by Oche et al [ 13 ]. The absorbance at 510 nm was recorded using a UV–vis spectrophotometer (Shimadzu, UVmini-1240, Japan).…”

Comparative Study of the Effects of Annona muricata and Tapinanthus globiferus Extracts on Biochemical Indices of Diabetic Rats

“…5,17,18 In other related works it was reported that AME and TGE could protect serum and brain lipids from peroxidation through the enhancement of antioxidant enzyme levels. [10][11][12][13][14][15][16][17][18] The most common lipid abnormalities in diabetes are hypertriglyceridemia and hypercholesterolemia. The increase in the levels of serum levels of cholesterol and triglycerides in the diabetic rats is a result of insulin deficiency and altered activity of enzymes such as lipases and lecithin cholesterol acyl transferase (LCAT) for regulation of blood lipids.…”

“…10 These pharmacological benefits could be explained by the rich antioxidant contents evidenced by the ability to scavenge free radicals and inhibit lipid peroxidation. 11 Therefore, the present study was designed to compare the benefits of the two plants in combating the complications of diabetes on one hand, and on the other hand establish the benefits or otherwise of the combination of both plants.…”

Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees

“…Hemiparasitic plants (Mistletoe) have been investigated for the absorption of nutrients, water, and metabolites from their respective hosts [31,32], and it has been revealed that the metabolic profile of Mistletoe changes significantly with the type of host plant, which affects its antioxidant activity [33][34][35] and could be ascribed to differences in polyphenolic contents [36]. For determining the antioxidant potential of the plant extracts, there is no specific evaluation method due to the structural diversity of compounds, their modes of action, and multiple patterns of interactions [37].…”