In vitro antioxidant activity on roots of Limnophila heterophylla

Sundararajan, Raja; Koduru, Ravindranadh

doi:10.5530/fra.2016.2.8

Cited by 16 publications

(12 citation statements)

References 29 publications

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“…Our results are in tune with the results reported by Sundararajan and Koduru, while studying the antioxidant activity of Limnophila heterophylla [41]. Furthermore, similar results were obtained by Saiah et al while analyzing the antioxidant activity of six Algerian medicinal plants [42].…”

Section: Superoxide Anion (Osupporting

confidence: 81%

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

Bhat

Ganie

Dar

et al. 2018

Asian J Pharm Clin Res

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Objectives: Taxus wallichiana, the member of family Taxaceae is used for the cure of various ailments by a large ethnic population native to the forests in Kashmir valley. The aim of this study is to evaluate antioxidant potential and hepatoprotective action of T. wallichiana as claimed by the ethnic population segment.Methods: Antioxidant potential of different T. wallichiana extracts was evaluated by estimating the total phenolics, 1,1-diphenyl -2-picrylhydrazyl (DPPH), reducing power, microsomal lipid peroxidation (LPO), and hydroxyl radical scavenging activity through standard protocols. Hepatoprotective activity of T. wallichiana was determined by evaluation of liver marker enzyme (aspartate transaminase, alanine transaminase, and alkaline phosphatase) levels using standard protocols and by histological analysis of rat liver tissues of different animal groups against CCl 4 induced liver damage.Results: Phenolic content expressed as gallic acid equivalents (GAE/g) was found maximum in case of ethanolic extract (153.34 mg GAE/g), followed by extracts of methanol (149.24 mg GAE/g), hexane (134 mg GAE/g), ethyl acetate (128.9 mg GAE/g), chloroform (121.37 mg GAE/g), and aqueous (113.00 mg GAE/g). At concentration of 700 µg/ml, DPPH radical scavenging activity of methanol extract was 88.29% with IC 50 (212.00 µg/ml), aqueous (82.47%) with IC 50 (258.29 µg/ml), and ethyl acetate (79.57%) with IC 50 (301.80%µg/ml). The reducing power of the extracts increased in a concentration dependent manner. At concentration of 700 µg/ml, 79%, 68%, and 56% inhibition was observed with extracts of methanol, aqueous, and ethyl acetate on microsomal LPO with IC 50 values of 126.09, 168.83, and151.96 µg/ml, respectively. Superoxide radical scavenging activity of T. wallichiana extracts increased in a dose-dependent manner with IC 50 values 170.30 µg/ml (methanol), 257.00 µg/ml (aqueous), and 297.55 µg/ml ethyl acetate extract. T. wallichiana extracts exhibited antioxidant effects on calf thymus DNA damage. The results further depict the hepatoprotective action of T. wallichiana extract at doses of 100 and 300 mg/kg and were comparable with that of standard treatment comprising 50 mg/kg-day of Vitamin C, a known hepatoprotective agent. The data were substantiated with histopathological studies of rat liver sections. Conclusion:These results comprehensively depict that T. wallichiana extracts are endowed with the free radical sequestering potential and be employed as potential antioxidant and hepatoprotective arsenal against many oxidative stress linked diseases.

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Section: Superoxide Anion (Osupporting

confidence: 81%

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

Bhat

Ganie

Dar

et al. 2018

Asian J Pharm Clin Res

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“…Our results corroborates with earlier reports. 24 In the present study, the qualitative and quantitative estimation of total phenolic and flavonoid contents of tartary buckwheat revealed that methanolic extract exhibits the highest concentration of total phenol and flavonoid content. Our results are in accordance with earlier reports where total flavonoid and phenolic content are found in higher amount in different parts.…”

Section: Discussionmentioning

confidence: 52%

“…Our results are in accordance with earlier reports where total flavonoid and phenolic content are found in higher amount in different parts. 25,24 It has been reported that plants rich in phenolic and flavonoids could be a potential source of therapeutics against oxidative stress. 26 Reducing power is often an indicator of antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Fingerprinting and Antioxidant Potential of Tartary Buckwheat- an Underutilized Pseudocereal crop from Kashmir Region

Pirzadah

Malik

Tahir

et al. 2016

FRA

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Objective: Buckwheat is an underutilized pseudocereal crop used as a staple food especially in Himalayan regions. The aim of the present study was to evaluate the phytochemical screening, antioxidant potential and metabolite profiling of tartary buckwheat extract. Methods: Tartary buckwheat leaf samples were tested for total phenols, flavonoids and in vitro antioxidant potential in terms of total antioxidant activity, free radical scavenging (superoxide, hydrogen peroxide) and DPPH assay. GC-MS profiling was done to identify and quantify the various metabolites from the methanolic leaf and groat extract. Results: Preliminary phytochemical screening of methanolic extract revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, phlobatannins, coumarins, glycosides and anthoquinones. Methanolic extract exhibits higher TPC (28.32 ± 5.31 mg gallic acid equivalent g -1 DW) and TFC (25.18 ± 3.5 mg rutin equivalent g -1 DW). DPPH radical (EC 50 =1.8 µg ml ) potential of tartary buckwheat leaf extract shows promising results. From GC-MS metabolite fingerprinting, over 111 and 24 metabolites were identified among leaf and groat extract respectively. The major compounds present in the extracts were fatty acids, hydrocarbons, steroids, terpenoids, esters, organic acids and aldehydes with excellent pharmaceutical properties. Conclusion:The tartary buckwheat extract were found to contain numerous metabolites with potent antioxidant and other pharmacological actions. Thus, tartary buckwheat could be a promising alternative in functional food sector to improve social well-being and neutraceutical to diminish malnutrition especially for the impoverished community.

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“…28 Our results are in tune with the results reported by Sundararajan and Koduru, while studying the antioxidant activity of Limnophila heterophylla. 29 It is well known that the vitality of cells depends on the integrity of their membranes. Exposure of red blood cell to injurious substances such as hypotonic medium and phenylhydrazine results in lysis of its membrane accompanied by haemolysis and oxidation of haemoglobin.…”

Section: Resultsmentioning

confidence: 99%

In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii

Tantary

Akbar

Bhat

et al. 2016

FRA

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Background: Euphorbia wallichii belongs to family Euphorbiaceae is used to treat various diseases on folklore levels in Kashmir valley. Objective of the study is to explore antioxidant potential and anti-inflammatory activities of Euphorbia wallichii. Materials and Methods: Antioxidant potential of extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal LPO, and hydroxyl radical scavenging activity by using standard procedures. Anti-inflammatory activity was assessed using hypotonic solution induced human erythrocyte haemolysis. Results: The highest phenolic content of 465 mg GAE/g was observed in methanol extract followed by ethyl acetate (359 mg GAE/g) and aqueous extract (291 mg GAE/g). At concentration of 700 µg/mL, DPPH radical scavenging activity of methanol extract was (94.85%) IC 50 (160 µg/ml), ethyl acetate (92.68%) IC 50 (200 µg/ml) and aqueous (90%) IC 50 (250 µg/ml). The reducing power of the extracts increased in a concentration dependent manner. At concentration of 70 µg/mL, 92.72, 80.74 and 75.75% inhibition was observed with methanol, ethyl acetate and aqueous extract on microsomal LPO with IC 50 values 31.5, 34.5 and 42 gµ/ml. Superoxide radical scavenging activity of Euphorbia wallichii extracts increased in a dose dependent manner with IC 50 values 36.05 µg/ml (methanol), 45 µg/ml (ethyl acetate) and 34.5 µg/ml (aqueous extract). Euphorbia wallichii extracts exhibited antioxidant effects on Calf thymus DNA damage. At the higher concentration of plant extracts (12 µg/ml), 90, 86 and 78% increase in RBC membrane stabilization was observed with methanol, ethyl acetate and aqueous extracts of Euphorbia wallichii. Conclusion: These results clearly indicate that Euphorbia wallichii extracts possesses the free radical savaging activity as such can be employed as potential antioxidant and anti-inflammatory agent against various oxidative stress related pathological conditions.

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In vitro antioxidant activity on roots of Limnophila heterophylla

Cited by 16 publications

References 29 publications

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

In Vitro Antioxidant Potential and Hepatoprotective Activity of Taxus Wallichiana

Metabolite Fingerprinting and Antioxidant Potential of Tartary Buckwheat- an Underutilized Pseudocereal crop from Kashmir Region

In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii

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