In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica

Peng, Zhenfei; Liu, Min; Fang, Zhexiang; Zhang, Qiqing

doi:10.1016/j.ijbiomac.2012.04.006

Cited by 38 publications

(15 citation statements)

References 25 publications

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“…Oxidation is an essential biological process to many organisms for the production of energy (Peng et al . ). Reactive oxygen species (ROS) are constantly formed in the human body and removed by antioxidant defense systems.…”

Section: Introductionmentioning

confidence: 97%

Enhanced in Vitro Antioxidant Activity of Polysaccharides From Enteromorpha Prolifera by Enzymatic Degradation

Zhou

et al. 2015

Journal of Food Biochemistry

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In order to improve the antioxidant activity of polysaccharide derived from Enteromorpha prolifera (PEP), PEP was hydrolyzed to relatively low molecular weight polysaccharides in the presence of either pectinase or gluco-amylase. Response surface methodology was employed to optimize the hydrolysis conditions based on the polysaccharide degradation rate. Optimum hydrolysis for pectinase was found to be: enzyme concentration 9.36 U/mL, reaction temperature 56C and pH 4.45; for gluco-amylase, the optimum conditions were: enzyme concentration 14.20 U/mL, reaction temperature 49C and pH 4.48. The enzymatic hydrolysates of polysaccharide obtained under optimal conditions were evaluated for their antioxidant activities by determining their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl, hydroxyl and superoxide anion radicals. The results indicate that the enzymatic hydrolysates of polysaccharide exhibited stronger free radical scavenging ability than nondegraded polysaccharide, while pectinase hydrolyzed polysaccharide was found to possess superior radical scavenging ability to that of gluco-amylase hydrolyzed polysaccharide. PRACTICAL APPLICATIONSIn order to fully utilize polysaccharide resources, an effective method to improve the antioxidant activity of Enteromorpha prolifera was established by enzymatic degradation. The hydrolysates obtained by this method may be used as ingredients of functional foods due to their antioxidant activity.

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Section: Introductionmentioning

confidence: 97%

Enhanced in Vitro Antioxidant Activity of Polysaccharides From Enteromorpha Prolifera by Enzymatic Degradation

Zhou

et al. 2015

Journal of Food Biochemistry

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“…Oxidation, caused by reactive oxygen species (ROS), is a pervasive biological process in physiology and metabolism of many organisms [1]. ROS are normally generated in the human body and scavenged by antioxidant defenses system when ROS remains at physiological concentrations [2].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and antioxidant properties of novel inulin derivatives with amino-pyridine group

Zhang

Chen

et al. 2014

International Journal of Biological Macromolecules

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a b s t r a c tA series of novel inulin derivatives were synthesized via reaction of chloracetyl inulin (CAIL) with aminopyridines, including 2-(2-amino-pyridyl)acetyl inulin chloride (2APAIL), 2-(3-amino-pyridyl)acetyl inulin chloride (3APAIL), 2-(4-amino-pyridyl)acetyl inulin chloride (4APAIL), 2-(2,3-diaminopyridyl)acetyl inulin chloride (2,3DAPAIL), and 2-(3,4-diamino-pyridyl)acetyl inulin (3,4DAPAIL). The antioxidant property of the products and 2-pyridylacetyl inulin chloride (PAIL) against hydroxyl radicals (• OH), superoxide radicals (O 2 • ), and DPPH radicals (DPPH • ) were evaluated in vitro, respectively. Results showed that 4APAIL and 3,4DAPAIL exhibited remarkable improvement on scavenging• OH and DPPH• , which can scavenge the radical of • OH completely at 0.4 mg/mL. Besides, the scavenging activity of 2,3DAPAIL to O 2• was excellent among all of the tested samples, reaching 85% at 1.6 mg/mL. These data indicate that all of the inulin derivatives have better antioxidant activities than inulin, and the scavenging effect indices are affected by the number and position of the amino group on pyridine grafted to the inulin derivatives.

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“…While PubMed shows 179 FCD publications in the decade from 1984 to 1993, there were 370 from 1994 to 2003, and 541 from 2004 to 2013. Numerous investigations revealed that FCD exhibits various biological effects, such as antimetastatic activity by blocking the interactions between cancer cells and the basement membrane [ 3 ]; induction of cancer cells apoptosis [ 4 ]; anticoagulant activity [ 5 ], antii-nflammation [ 6 , 7 ]; antimicrobial activity [ 8 ]; and antioxidation [ 9 , 10 ]. These findings have stimulated an explosion of investigations on FCD, its bioactivities and its possible role in human health.…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Supplementation Improves Exercise Performance and Exhibits Anti-Fatigue Action in Mice

Chen

Tsai

et al. 2014

Nutrients

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Fucoidan (FCD) is a well-known bioactive constituent of seaweed extract that possess a wide spectrum of activities in biological systems, including anti-cancer, anti-inflammation and modulation of immune systems. However, evidence on the effects of FCD on exercise performance and physical fatigue is limited. Therefore, we investigated the potential beneficial effects of FCD on ergogenic and anti-fatigue functions following physiological challenge. Male ICR mice from three groups (n = 8 per group) were orally administered FCD for 21 days at 0, 310 and 620 mg/kg/day, which were, respectively, designated the vehicle, FCD-1X and FCD-2X groups. The results indicated that the FCD supplementations increased the grip strength (p = 0.0002) and endurance swimming time (p = 0.0195) in a dose-depend manner. FCD treatments also produced dose-dependent decreases in serum levels of lactate (p < 0.0001) and ammonia (p = 0.0025), and also an increase in glucose level (p < 0.0001) after the 15-min swimming test. In addition, FCD supplementation had few subchronic toxic effects. Therefore, we suggest that long-term supplementation with FCD can have a wide spectrum of bioactivities on health promotion, performance improvement and anti-fatigue.

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In vitro antioxidant effects and cytotoxicity of polysaccharides extracted from Laminaria japonica

Cited by 38 publications

References 25 publications

Enhanced in Vitro Antioxidant Activity of Polysaccharides From Enteromorpha Prolifera by Enzymatic Degradation

Enhanced in Vitro Antioxidant Activity of Polysaccharides From Enteromorpha Prolifera by Enzymatic Degradation

Synthesis, characterization, and antioxidant properties of novel inulin derivatives with amino-pyridine group

Fucoidan Supplementation Improves Exercise Performance and Exhibits Anti-Fatigue Action in Mice

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