In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita

Taju, G.; Majeed, Shahid; Nambi, K.S.N.; Hameed, A.S. Sahul

doi:10.1016/j.cbpc.2014.01.007

Cited by 60 publications

(31 citation statements)

References 52 publications

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“…However, at higher concentrations of TiO 2 NPs, the CAT activity significantly decreased. A similar decrease in SOD, CAT and GSH content was observed in SICH, ICG and LRG cells exposed to silver nanoparticles (Ag-NPs) in a dose-dependent manner at higher concentrations (Taju et al 2014). The decline in GSH content is the result of detoxification by the ROS generated due to TiO 2 NPs.…”

Section: Discussionmentioning

confidence: 69%

Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells

Srikanth

Pereira

Duarte

et al. 2015

Environ Sci Pollut Res

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Titanium oxide nanoparticles (TiO2 NPs) have received wide attention in diverse application, but the potential impact of these nanomaterials on the environment, aquatic life and especially on fish cell lines is lacking. The present study aimed to investigate the cytotoxicity and oxidative stress induced by TiO2 NPs on Chinook salmon cells derived from Oncorhynchus tshawytscha embryos (CHSE-214). The The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] and neutral red (NR) assays in CHSE-214 cells exposed to TiO2 NPs revealed concentration-dependent cytotoxic effect in the range of 10 to 60 μg/ml for 24 h. CHSE-214 cells exposed to TiO2 NPs (10-60 μg/ml) exhibited significant decline in superoxide dismutase (SOD), catalase (CAT) glutathione (GSH) content and increased lipid peroxidation (LPO) in a concentration-dependent manner. TiO2 NPs induced cytotoxicity and oxidative stress in CHSE-214 cells which serve as a base line studies for future studies.

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Section: Discussionmentioning

confidence: 69%

Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells

Srikanth

Pereira

Duarte

et al. 2015

Environ Sci Pollut Res

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“…Increased H 2 O 2 levels resulting from CAT inhibition could ultimately further inhibit SOD activity (Kono and Fridovich, 1982). The combined inhibition of SOD and CAT, together with those of both GPxs, could lead to a severe accumulation of oxyradicals owing to the decrease of detoxifying activities and increase of ROS production (Ahn et al, 2014;Taju et al, 2014). However, compensatory mechanisms between antioxidants may occur.…”

Section: Discussionmentioning

confidence: 98%

Bioaccumulation and oxidative stress responses measured in the estuarine ragworm (Nereis diversicolor) exposed to dissolved, nano- and bulk-sized silver

Cozzari

Elia

Pacini

et al. 2015

Environmental Pollution

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“…It is well known that ionic Ag is one of the most toxic metals for aquatic organisms and that invertebrates are sensitive to very low concentrations of this metal [4]. Other studies have also shown that ionic Ag is more toxic than Ag NPs in vivo [11, 50, 51] and in vitro [15, 51–53]. Bulk Ag showed a relatively lower toxicity to mussel cells as well as in other studies with human cells [54] and fish cell lines [50].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of Toxicity of Ag Nanoparticles in Comparison to Bulk and Ionic Ag on Mussel Hemocytes and Gill Cells

et al. 2015

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Silver nanoparticles (Ag NPs) are increasingly used in many products and are expected to end up in the aquatic environment. Mussels have been proposed as marine model species to evaluate NP toxicity in vitro. The objective of this work was to assess the mechanisms of toxicity of Ag NPs on mussel hemocytes and gill cells, in comparison to ionic and bulk Ag. Firstly, cytotoxicity of commercial and maltose stabilized Ag NPs was screened in parallel with the ionic and bulk forms at a wide range of concentrations in isolated mussel cells using cell viability assays. Toxicity of maltose alone was also tested. LC50 values were calculated and the most toxic Ag NPs tested were selected for a second step where sublethal concentrations of each Ag form were tested using a wide array of mechanistic tests in both cell types. Maltose-stabilized Ag NPs showed size-dependent cytotoxicity, smaller (20 nm) NPs being more toxic than larger (40 and 100 nm) NPs. Maltose alone provoked minor effects on cell viability. Ionic Ag was the most cytotoxic Ag form tested whereas bulk Ag showed similar cytotoxicity to the commercial Ag NPs. Main mechanisms of action of Ag NPs involved oxidative stress and genotoxicity in the two cell types, activation of lysosomal AcP activity, disruption of actin cytoskeleton and stimulation of phagocytosis in hemocytes and increase of MXR transport activity and inhibition of Na-K-ATPase in gill cells. Similar effects were observed after exposure to ionic and bulk Ag in the two cell types, although generally effects were more marked for the ionic form. In conclusion, results suggest that most observed responses were due at least in part to dissolved Ag.

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In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita

Cited by 60 publications

References 52 publications

Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells

Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells

Bioaccumulation and oxidative stress responses measured in the estuarine ragworm (Nereis diversicolor) exposed to dissolved, nano- and bulk-sized silver

Mechanisms of Toxicity of Ag Nanoparticles in Comparison to Bulk and Ionic Ag on Mussel Hemocytes and Gill Cells

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