In vitro-based doubled haploid production: recent improvements

Niazian, Mohsen; Shariatpanahi, Mehran E.

doi:10.1007/s10681-020-02609-7

Cited by 68 publications

(49 citation statements)

References 104 publications

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“…Thus, the breeding time can be reduced, accelerating varietal improvement since bamboo's vegetative growth phase is too long. However, this method also varied between genotypes and developmental stage of immature pollen grain/ microspores (Niazian & Shariatpanahi, 2020). Due to this varying clonal requirement, development of a common protocol for large‐scale and efficient in vitro regeneration has been a major bottleneck in micropropagation of bamboo (Ye et al., 2017).…”

Section: Transgenic Approaches To Crop Improvementmentioning

confidence: 99%

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

Ramakrishnan

Yrjälä

Vinod

et al. 2020

Food and Energy Security

101

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Section: Transgenic Approaches To Crop Improvementmentioning

confidence: 99%

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

Ramakrishnan

Yrjälä

Vinod

et al. 2020

Food and Energy Security

101

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“…Among the first examples, prior to the 1930s, corn ( Zea mays ) was an open pollinated crop that displayed a high degree of variation but has since transitioned to the F1 hybrid seed that is over 99% genetically uniform [ 10 ]. In addition to greater uniformity, this transition has resulted in increased yields, stress tolerance, and overall productivity [ 11 ]. In the case of cannabis, transitioning to F1 hybrid seed production might lead to similar achievements as well as help to ensure a consistently low level of THC to meet regulatory requirements.…”

Section: Introductionmentioning

confidence: 99%

“…This process is challenging in cannabis as it is a dioecious plant meaning the male and female reproductive systems occur on separate plants. Researchers have developed methods to overcome this challenge by applying various chemical compounds, such as gibberellic acid, silver nitrate, or silver thiosulphate, to induce male flowers on female plants [ 11 ]. This method facilitates the production of inbred lines as well as of feminized seeds (genetically female seeds).…”

Section: Introductionmentioning

confidence: 99%

“…Feminized seeds are ideal for hemp cultivation to produce cannabinoids. While this approach provides an interesting solution to overcome the dioecy in cannabis plants, it is time-consuming and there is a belief that repeated artificial induction of male flowers results in an increased rate of hermaphroditism in the offspring [ 11 , 12 ]. Although the increased prevalence of hermaphroditism has yet to be evaluated, it is theoretically plausible through epigenetic mechanisms [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Synergizing Off-Target Predictions for In Silico Insights of CENH3 Knockout in Cannabis through CRISPR/Cas

Hesami

Yoosefzadeh-Najafabadi

Adamek

et al. 2021

Molecules

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The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-mediated genome editing system has recently been used for haploid production in plants. Haploid induction using the CRISPR/Cas system represents an attractive approach in cannabis, an economically important industrial, recreational, and medicinal plant. However, the CRISPR system requires the design of precise (on-target) single-guide RNA (sgRNA). Therefore, it is essential to predict off-target activity of the designed sgRNAs to avoid unexpected outcomes. The current study is aimed to assess the predictive ability of three machine learning (ML) algorithms (radial basis function (RBF), support vector machine (SVM), and random forest (RF)) alongside the ensemble-bagging (E-B) strategy by synergizing MIT and cutting frequency determination (CFD) scores to predict sgRNA off-target activity through in silico targeting a histone H3-like centromeric protein, HTR12, in cannabis. The RF algorithm exhibited the highest precision, recall, and F-measure compared to all the tested individual algorithms with values of 0.61, 0.64, and 0.62, respectively. We then used the RF algorithm as a meta-classifier for the E-B method, which led to an increased precision with an F-measure of 0.62 and 0.66, respectively. The E-B algorithm had the highest area under the precision recall curves (AUC-PRC; 0.74) and area under the receiver operating characteristic (ROC) curves (AUC-ROC; 0.71), displaying the success of using E-B as one of the common ensemble strategies. This study constitutes a foundational resource of utilizing ML models to predict gRNA off-target activities in cannabis.

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“…Plants respond to harsh environments by applying an array of tolerance mechanisms. Cellular detoxification activation, osmoprotectants accumulation, antioxidant machinery activation (both enzymatic and non-enzymatic), signaling pathways activation/regulation, and ROS scavenging are involved in enhancing plant stress tolerance (Niazian and Shariatpanahi, 2020 ; Shailani et al, 2020 ). Cell osmotic pressure adjustment and the protection of cell membranes and photosynthesis machinery coupled with other functional macromolecules are vital measures to eliminate the harmful effects of abiotic stresses (Niazian et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Betaine Aldehyde Dehydrogenase (BADH) vs. Flavodoxin (Fld): Two Important Genes for Enhancing Plants Stress Tolerance and Productivity

et al. 2021

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Abiotic stresses, mainly salinity and drought, are the most important environmental threats that constrain worldwide food security by hampering plant growth and productivity. Plants cope with the adverse effects of these stresses by implementing a series of morpho-physio-biochemical adaptation mechanisms. Accumulating effective osmo-protectants, such as proline and glycine betaine (GB), is one of the important plant stress tolerance strategies. These osmolytes can trigger plant stress tolerance mechanisms, which include stress signal transduction, activating resistance genes, increasing levels of enzymatic and non-enzymatic antioxidants, protecting cell osmotic pressure, enhancing cell membrane integrity, as well as protecting their photosynthetic apparatus, especially the photosystem II (PSII) complex. Genetic engineering, as one of the most important plant biotechnology methods, helps to expedite the development of stress-tolerant plants by introducing the key tolerance genes involved in the biosynthetic pathways of osmolytes into plants. Betaine aldehyde dehydrogenase (BADH) is one of the important genes involved in the biosynthetic pathway of GB, and its introduction has led to an increased tolerance to a variety of abiotic stresses in different plant species. Replacing down-regulated ferredoxin at the acceptor side of photosystem I (PSI) with its isofunctional counterpart electron carrier (flavodoxin) is another applicable strategy to strengthen the photosynthetic apparatus of plants under stressful conditions. Heterologous expression of microbially-sourced flavodoxin (Fld) in higher plants compensates for the deficiency of ferredoxin expression and enhances their stress tolerance. BADH and Fld are multifunctional transgenes that increase the stress tolerance of different plant species and maintain their production under stressful situations by protecting and enhancing their photosynthetic apparatus. In addition to increasing stress tolerance, both BADH and Fld genes can improve the productivity, symbiotic performance, and longevity of plants. Because of the multigenic and complex nature of abiotic stresses, the concomitant delivery of BADH and Fld transgenes can lead to more satisfying results in desired plants, as these two genes enhance plant stress tolerance through different mechanisms, and their cumulative effect can be much more beneficial than their individual ones. The importance of BADH and Fld genes in enhancing plant productivity under stress conditions has been discussed in detail in the present review.

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In vitro-based doubled haploid production: recent improvements

Cited by 68 publications

References 104 publications

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry

Synergizing Off-Target Predictions for In Silico Insights of CENH3 Knockout in Cannabis through CRISPR/Cas

Betaine Aldehyde Dehydrogenase (BADH) vs. Flavodoxin (Fld): Two Important Genes for Enhancing Plants Stress Tolerance and Productivity

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