In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var. Amaou)

Zhu, Qing; Nakagawa, Toshinori; Kishikawa, Asuka; Ohnuki, Koichiro

doi:10.1016/j.jff.2014.12.026

Cited by 61 publications

(55 citation statements)

References 42 publications

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“…The inhibition of tyrosinase was determined in a cell-free system using mushroom tyrosinase using the method reported by Aoki, Tanigawa, Abe, and Fujiwara (2007) and Zhu, Nakagawa, Kishikawa, Ohnuki, and Shimizu (2015), with slight modifications. One thousand units of mushroom tyrosinase in 0.1M phosphate buffer at pH 6.8 and samples or AsA were coincubated for 10 min at 37°C.…”

Section: Measurement Of Mushroom Tyrosinase Activitymentioning

confidence: 99%

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Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

Chang

Dai

Leu

et al. 2015

Journal of Functional Foods

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Section: Measurement Of Mushroom Tyrosinase Activitymentioning

confidence: 99%

“…Tyrosinase activity was determined using absorbance at 475 nm in the reaction mixture, and expressed as a percentage of the control value. (Zhu et al, 2015) 2.5.…”

Section: Measurement Of Mushroom Tyrosinase Activitymentioning

confidence: 99%

Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

Chang

Dai

Leu

et al. 2015

Journal of Functional Foods

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“…The free radical scavenging ability of the mushroom extracts was also tested using the ABTS assay, following the method described by Zhu et al [22]. The ABTS radical cation (ABTS ? )…”

Section: Abts Assaymentioning

confidence: 99%

Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

et al. 2016

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Mushrooms have garnered immense popularity for their nutritional as well as medicinal values. The therapeutic potential of mushrooms in Nepal, a country well known for its biodiversity and natural medicinal resources, remains largely unstudied. Therefore, this study attempts to unveil the antioxidative properties of Nepalese wild mushrooms. Sixty-two wild mushroom samples were collected from several forests in different parts of Nepal. Ethanol and water extracts of the dried samples were tested for their antioxidative activities using total phenolic content (TPC), oxygen radical absorbance capacity (ORAC) assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power (RP) assays. Ethanol extracts of samples belonging to the order Hymenochaetales showed significantly high activity in all the assays. Inonotus clemensiae had an exceptionally high TPC of 643.2 mg gallic acid equivalent (GAE)/g extract and also exhibited the lowest EC50 values in DPPH (0.081 mg/mL), ABTS (0.409 mg/mL), and EC0.5 value in reducing power (RP; 0.031 mg/mL) assays. High-performance liquid chromatography (HPLC) analysis of the top ten samples with the highest TPC was done to identify the phenolic compounds in the extracts, followed by liquid chromatography-mass spectrometry (LC-MS) analysis for some unknown compounds. These findings highlight the very strong antioxidative activity of Nepalese mushrooms, and paves the way for further research to explore their economic potential.

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“…as whitening agents), and agriculture (as bioinsecticides) by inhibiting enzymatic oxidation [4,5]. Tyrosinase inhibitor is one of the candidates for reduction of melanogenesis [6][7][8]. The majority of tyrosinase inhibitors that have been identified use phenolic or metal-chelating structures in their activities.…”

Section: Introductionmentioning

confidence: 99%

Antitumoral, antioxidant, and antimelanogenesis potencies of Hawthorn, a potential natural agent in the treatment of melanoma

et al. 2016

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The lack of an efficient agent that does not have the disadvantage of low activity (kojic acid), high cytotoxicity, and mutagenicity (hydroquinone), poor skin penetration (arbutin), or low stability in formulation (glabridin) led us to continue our research on new antipigmentation/skin-lightening agents. Therefore, research of natural products that can modulate the metabolism of pigmentation is of great interest. Otherwise, malignant melanoma is one of the most aggressive forms of skin cancer, with high metastatic potential, and currently, there is no effective chemotherapy against invasive melanoma. Therefore, it is necessary to develop new drugs with potent activity and weak side effects against melanoma. The in-vitro anticancer effect of hawthorn was analyzed against B16F10 melanoma cells using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The effect of isolated compounds from hawthorn on melanogenesis in B16F10 melanoma cells was investigated by measuring the amounts of melanin and tyrosinase spectrophotometrically at 475 nm. Balb/c mice models inoculated with B16F10 mouse tumor cells were used to evaluate the in-vivo antitumoral potential of hawthorn by assessing its effect on the growth of transplanted tumors. The antioxidant potential of tested samples was evaluated in B16F10 and primary human keratinocyte cells using a cellular antioxidant activity assay. Hawthorn tested samples inhibited effectively the growth of melanoma cells in vitro. Furthermore, it appears that tested samples from hawthorn reduced melanogenesis by inhibiting the tyrosinase activity of B16F10 cells in a dose-dependent manner. In-vivo studies showed that hawthorn total oligomer flavonoids extract treatment at a dose of 150 mg/kg body weight for 21 days in implanted tumor mice resulted in significant inhibition of the tumor growth volume and weight. In addition, tested samples showed significant cellular antioxidant capacity against the reactive oxygen species in B16F10 and primary human keratinocyte cells. Our results indicate that hawthorn could be considered as a promising agent for the treatment of melanoma as it shows antitumor activity in vitro and in vivo. Moreover, hawthorn constituents are shown to be highly effective at inhibiting tyrosinase-mediated melanogenesis in vitro on melanoma cells by preventing oxidation in these cells and without affecting the viability of normal human keratinocyte cells. Then, hawthorn might also be used as a new candidate of natural skin depigmenting agents in skin care products.

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In vitro bioactivities and phytochemical profile of various parts of the strawberry (Fragaria × ananassa var. Amaou)

Cited by 61 publications

References 42 publications

Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

Effects of the melanogenic inhibitor, uracil, derived from Lactobacillus plantarum TWK10-fermented soy milk on anti-melanogenesis in B16F0 mouse melanoma cells

Antioxidative activities of 62 wild mushrooms from Nepal and the phenolic profile of some selected species

Antitumoral, antioxidant, and antimelanogenesis potencies of Hawthorn, a potential natural agent in the treatment of melanoma

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