In vitro bioactivity and stem cells attachment of three-dimensionally ordered macroporous bioactive glass incorporating iron oxides

Abstract

Three dimensionally ordered macroporous bioactive SiO 2 CaO Na 2 O P 2 O 5 glass (3DOM BG) is synthesized by using the sol gel method. After an in vitro test in simulated body fluid (SBF), the hydroxyapatite (HAp) crystalline phase is clearly formed on its surface as confirmed by X ray diffractometry (XRD) and Raman spectroscopy. Mag netic 3DOM BG/Fe samples are synthesized by partial substitution of SiO 2 with iron oxide. Whilst the HAp layer is not confirmed, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR) and XRD analysis reveal calcium phosphate layer on the surface of 3DOM BG/Fe samples after the SBF soaking. The growth of HAp like layer is slower with increasing iron oxides. The initial mechanism that thought to induce bone forma tion is reduced due to the replacement of Ca 2+ with Fe ions in the glass network. The formation of HAp like layer is modified by the sedimentation of Ca and P while the nonmagnetic 3DOM BG forms the calcium phosphate by the ionic exchange following the Hench mechanism. The adult human adipose tissue derived stem cells (hADSCs) can be closely attached and well spread on the flat plate of all 3DOM BG/Fe and 3DOM BG. Without detectable cytotoxicity possibly induced by iron oxides, the osteoblast can be grown and proliferated. In addition to these bioactivity and biocompatibility, porous structures can allow their possible use in targeted drug delivery and magnetic properties of 3DOM BG/Fe can essentially be implemented in hyperthermia therapy.

Preparation and In Vitro Osteogenic Evaluation of Biomimetic Hybrid Nanocomposite Scaffolds Based on Gelatin/Plasma Rich in Growth Factors (PRGF) and Lithium-Doped 45s5 Bioactive Glass Nanoparticles

“…2 e–l) [ 12 , 70 ]. Other researchers have confirmed these findings [ 102 , 103 ]. There is much interest today in using PRGF for bone repair in dentistry applications [ 104 ].…”

An Investigation on Spray-Granulated, Macroporous, Bioactive Glass Microspheres for a Controlled Drug Delivery System

“…Initially, Figure 4 a shows the FTIR spectra of all BG specimens before SBF immersion. The resulting peaks at 1095, 802 and 482 cm −1 can be observed, which correspond to various Si–O–Si stretching and the bending bonds of the SiO 2 tetrahedral structure [ 37 , 38 ]. In contrast, the FTIR spectra after SBF immersion are shown in Figure 4 b.…”

Recent advances and future perspectives of sol–gel derived porous bioactive glasses: a review

“…279 Of course, the bone-bonding ability of bioactive glasses depends on biodegradability, the dissolution rate of its ions, formation of new bone tissues and the stimulation of osteoblasts upon soaking in SBF. 280 The inclusion of iron oxide is beneficial because it does not induce cytotoxicity and the osteoblast can be grown and proliferated. However, the growth of the HA like layer was slower with increasing iron oxide content.…”