In vitro BMP-2 peptide release from thiolated chitosan based hydrogel

Liu, Xujie; Yu, Bo; Huang, Qianli; Li, Rui; Feng, Qingling; Cai, Qiang; Mi, Shengli

doi:10.1016/j.ijbiomac.2016.08.048

Cited by 42 publications

(20 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The given timings were 1 h, 3 h, 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 9 days, 11 days, 13 days, 15 days, 17 days, and 19 days. The concentration of peptides in the release medium was determined with the bicinchoninic acid (BCA) protein assay kit (Biyuntian, China) according to the manufacturer's instructions …”

Section: Methodsmentioning

confidence: 99%

“…The concentration of peptides in the release medium was determined with the bicinchoninic acid (BCA) protein assay kit (Biyuntian, China) according to the manufacturer's instructions. 23 Isolation and culture of rat BMSCs This study was approved by The Institutional Animal Care and Use Committee of Zhejiang University (Hangzhou, China). Three-week-old male Sprague-Dawley (SD) rats were sacrificed for the isolation of BMSCs.…”

Section: Degradation In Vitromentioning

confidence: 99%

See 1 more Smart Citation

BMP2‐mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis

Miao

et al. 2019

J Biomed Mater Res

View full text Add to dashboard Cite

Commercial bone substitute, such as calcined bovine bone (CBB), is currently extensively used as an alternative to autogenous bone. However, CBB lacks osteoinductivity and merely serves as a scaffold for native bone formation. To address this issue, we designed and prepared a heptaglutamate (E7)‐modified BMP2‐mimicking peptide (7E) and carried out a series of comprehensive physical characterizations and in vivo and in vitro studies to evaluate its role in the repair of cranial defects. The data elucidated that the amount of peptide anchoring to the bone graft materials was remarkably increased after modified with E7. Of note, 7E had a relatively stable and durable release, which promoted the osteogenic differentiation of rat derived bone marrow mesenchymal stem cells (BMSCs) and enhanced the bone regeneration of a rabbit calvarial defect by regulating the expression of the Runx2/SP7 axis. In summary, the composite biomaterials incorporating the E7‐modified BMP2‐mimicking peptide and CBB prepared in this study is a novel bone augmentation material with the merits of non‐immunotoxicity, convenience, and low cost. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:80–93, 2020.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Degradation In Vitromentioning

confidence: 99%

BMP2‐mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis

Miao

et al. 2019

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…Compared with ordinary chitosan, sulfhydrylated chitosan has better tissue adhesion, stability, and protein release properties (Matsuda et al, 2005). A recent study showed a chitosan-4thiobutylamidine (CS-TBA) hydrogel prepared by sulfhydryl chitosan (Liu et al, 2016;Chen et al, 2017) has the following advantages: 1) self-sterilization: reduce the risk of surgical contamination; 2) low cost: can be synthesized in a large scale; 3) injectable: it is a liquid at room temperature, and it can quickly gel under the conditions in vivo (higher temperature); 4) high compatibility: can carry a variety of biological factors and composite materials, and has a wide range of applications (Liu et al, 2016;Chen et al, 2017). Therefore, the CS-TBA hydrogel could be a suitable candidate as the factor carrier for rotator cuff repair.…”

Section: Introductionmentioning

confidence: 99%

A Dual-Factor Releasing Hydrogel for Rotator Cuff Injury Repair

et al. 2021

View full text Add to dashboard Cite

Rotator cuff injury causes pain in the shoulder and is a challenge to be repaired even after surgical reconstruction. Here, we developed a dual-factor releasing hydrogel based on sulfhydrylated chitosan to deliver KGN and FGF-2 to the injured area to enable fast healing of the tendon–bone interface, which is essential for the repair of rotator cuff injury. We found that the two factors could be easily loaded into the hydrogel, which could in turn continuously release the factors in physiological conditions. The hydrogel was found to be a porous structure through a scanning electron microscope (SEM). The micropores in the hydrogel structure enable the loading and releasing of these molecules. This study showed that KGN and FGF-2 could play a synergistic effect by recruiting and promoting stem cell proliferation and chondrogenesis, thus accelerating the healing of the tendon–bone interface. An in vivo study based on a rabbit rotator cuff injury model demonstrated that the dual-factor releasing hydrogel possesses superior repair capacity than a single-factor releasing hydrogel and the untreated groups. In conclusion, the KGN and FGF-2 dual-factor releasing hydrogel could be a promising biomaterial for the regeneration of the tendon–bone interface and rotator cuff injury repair.

show abstract

“…A synthetic peptide corresponding to the residues 73-92 of the knuckle epitope of BMP-2 has been shown to stimulate bone precursor cells to induce calcification [17]. Furthermore, the use of this mimetic peptide encapsulated in thermosensitive hydrogels for minimally invasive surgery of bone repair was taken into consideration [18,19].…”

Section: Introductionmentioning

confidence: 99%

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

et al. 2020

View full text Add to dashboard Cite

Extensive efforts were undertaken to develop suitable biomaterials for tissue engineering (TE) applications. To facilitate clinical approval processes and ensure the success of TE applications, bioinspired concepts are currently focused on. Working on bone tissue engineering, we describe in the present study a method for biofunctionalization of collagen/hydroxyapatite composites with BMP-2 mimetic peptides. This approach is expected to be fundamentally transferable to other tissue engineering fields. A modified BMP-2 mimetic peptide containing a negatively charged poly-glutamic acid residue (E7 BMP-2 peptide) was used to bind positively charged hydroxyapatite (HA) particles by electrostatic attraction. Binding efficiency was biochemically detected to be on average 85% compared to 30% of BMP-2 peptide without E7 residue. By quartz crystal microbalance (QCM) analysis, we could demonstrate the time-dependent dissociation of the BMP-2 mimetic peptides and the stable binding of the E7 BMP-2 peptides on HA-coated quartz crystals. As shown by immunofluorescence staining, alkaline phosphatase expression is similar to that detected in jaw periosteal cells (JPCs) stimulated with the whole BMP-2 protein. Mineralization potential of JPCs in the presence of BMP-2 mimetic peptides was also shown to be at least similar or significantly higher when low peptide concentrations were used, as compared to JPCs cultured in the presence of recombinant BMP-2 controls. In the following, collagen/hydroxyapatite composite materials were prepared. By proliferation analysis, we detected a decrease in cell viability with increasing HA ratios. Therefore, we chose a collagen/hydroxyapatite ratio of 1:2, similar to the natural composition of bone. The following inclusion of E7 BMP-2 peptides within the composite material resulted in significantly elevated long-term JPC proliferation under osteogenic conditions. We conclude that our advanced approach for fast and cost-effective scaffold preparation and biofunctionalization is suitable for improved and prolonged JPC proliferation. Further studies should prove the functionality of composite scaffolds in vivo.

show abstract

In vitro BMP-2 peptide release from thiolated chitosan based hydrogel

Cited by 42 publications

References 36 publications

BMP2‐mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis

BMP2‐mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis

A Dual-Factor Releasing Hydrogel for Rotator Cuff Injury Repair

Establishment of Collagen: Hydroxyapatite/BMP-2 Mimetic Peptide Composites

Contact Info

Product

Resources

About