In Vitro Callus Development on Immature Leaf Explants of Liberica Coffee (Coffea liberica L. cv. Liberika Tungkal Komposit) by the Application of 2.4-D and BAP

Lizawati, Lizawati; Zulkarnain, Zulkarnain; Neliyati, Neliyati

doi:10.24252/bio.v8i2.11769

Cited by 2 publications

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“…The most successful callus formation achieved in the treatment with independently 2,4-D addition (T 0 D 1 , T 0 D 2 , T 0 D 3 ) and treatment with 10% tomato extract combined with auxin type 2,4-D (T 2 D 1 , T 2 D 2 , and T 2 D 3 ). Similar results were obtained by Lizawati et al (2020), which demonstrated a 100% success rate for callus formation across all treatments, including a single treatment of 2,4-D without cytokinin. According to Oliveira et al (2022), the auxin 2,4-D has been widely recognized for its effectiveness in initiating the callus induction stage in the somatic embryogenesis of coffee plants.…”

Section: Percentage Of Callus Formationsupporting

confidence: 79%

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

Fahira,

Latunra,

Johannes

2023

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Robusta coffee (Coffea canephora) is Indonesia's essential leading trade commodity. Robusta coffee is preferred for its bitter taste. However, robusta coffee productivity decreased due to bad agriculture practice. Tissue culture is an alternative to cultivation with various advantages. The plant growth regulators (PGRs) influence tissue culture’s success. Due to its stability, 2,4 dichlorophenoxyacetic acid has become a common synthetic PGRs. PGRs can be obtained from natural ingredients, such as tomatoes. This study aimed to analyze the effect of 2,4-dichlorophenoxyacetic acid and tomato extract addition and its appropriate concentration for callus induction of robusta coffee in vitro. This research was conducted at the Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This study used a Complete Randomized Design with two factors. The first factor was the concentration of tomato extract (0%; 7.5%; 10%; and 12.5%). Meanwhile, the second factor was the concentration of 2,4 dichlorophenoxyacetic acid (0 ppm; 1 ppm; 2 ppm; and 3 ppm). Observation parameters include the percentage of callus formation, callus growing time, callus fresh weight, callus color, and callus texture. The quantitative data were analyzed by the Kruskal-Wallis test followed by the Mann-Whitney Test to compare the effect of each treatment. The results showed that among other treatment combinations, treatment with the addition of 2 ppm 2,4-D and 10% tomato extract (T2D2) and treatment with the addition of 3 ppm 2,4-D and 10% tomato extract (T2D3) had the best effect in inducing the callus of robusta coffee.

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Section: Percentage Of Callus Formationsupporting

confidence: 79%

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

Fahira,

Latunra,

Johannes

2023

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“…It is easy to grow in lowlands area (< 800 m above sea level). Tungkal Composite of Liberica is a type of liberica coffee from Tanjung Jabung Timur Regency, Jambi Province [4] which has been released as a national superior variety. Currently the demand for this coffee tends to increase so that it is necessary to expand the planting area to increased production.…”

Section: Introductionmentioning

confidence: 99%

The effect of 2,4-D, BA and Thidiazuron on somatic embryo induction of liberica coffee of Tungkal Composite from Jambi

et al. 2023

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The Tungkal Composite of liberica coffee rejuvenation program at Jambi Province is urgently needed due to the latest status of the plants are old and showing diseased attacked. To support the rejuvenation, the provision of seedlings could be done by tissue culture approach through somatic embryogenesis. This research aimed to study the effect of 2,4-D, BA and TDZ on callus formation and embryo induction from liberica coffee leaf explants. Explants used were leaf sections with or without veins. Explants were grown on Murashige Skoog (MS) basal medium with 2,4-D + BA and 2,4-D + TDZ. A completely randomized design was employed in the trial, and each experimental unit was replicated three times. Results showed that callus could be formed on both types of explants, but leaf explants with veins could produce callus faster. The use of BA in medium could induce callus formation faster than TDZ. The higher the concentration of 2,4-D, BA and TDZ, the slower callus initiation. The addition of TDZ in the medium resulted in mostly callus, while callus from medium with BA showed a friable structure. Globular somatic embryos were formed in all treatments with varying amounts. TDZ can induce more globular somatic embryo than BA.

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In Vitro Callus Development on Immature Leaf Explants of Liberica Coffee (Coffea liberica L. cv. Liberika Tungkal Komposit) by the Application of 2.4-D and BAP

Cited by 2 publications

References 0 publications

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

Callus formation of Coffea canephora induced with 2,4 dichlorophenoxyacetic acid and tomato extract supplements

The effect of 2,4-D, BA and Thidiazuron on somatic embryo induction of liberica coffee of Tungkal Composite from Jambi

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