In vitro cell irradiation systems based on alpha source: construction and characterisation

Szabó, J.; Feher, Ioana; Palfálvi, J.; Balásházy, Imre; Dám, Annamária; Polonyi, István; Bogdándi, Enikő Noémi

doi:10.1016/s1350-4487(02)00089-6

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Such particles lose part of the energy they carry, in gold, air and Mylar layers, and then deposit the dose in the cell. The energy loss can be simulated using the SRIM program [5]. Calculations were made for three examples of the angle measured: 0°, 30° and 60°.…”

Section: Alpha Energy Spectrometrymentioning

confidence: 99%

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Radiobiological Research and Dosimetry Using a Flat Alpha Source

Szefliński¹,

Filipek²,

Gotlib³

et al. 2020

RAP 2019 Conference Proceedings

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The irradiation system consisting of an α-source and disc holder has been developed in the Heavy Ion Laboratory, University of Warsaw. A simple exposure system for irradiation of biological samples consists of the Am-241 disc source, source holder and biological samples cultured in special Petri dishes. The irradiation system has been investigated to determine the alpha spectrum and dose distribution in irradiated single cell layer attached to the Mylar foil. Commercial Am-241 disc source of 50 mm in diameter, with a radioactive element embedded into a substrate layer was examined to established the uniformity of surface radioactivity over the disc source. The experimental device is equipped with cell dishes of 40 mm in diameter and a 6 µm thick Mylar foil bottom. Care was taken for homogenous irradiation of the cells. Dose calibration for the irradiation system was calculated taking into account source-to-target geometry.

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Section: Alpha Energy Spectrometrymentioning

confidence: 99%

“…Similar exposure apparatus for in vitro irradiation using Am-241 α-source were constructed previously [4]. Irradiation systems with collimators providing an almost parallel α-particle beam and to provide a perpendicular angle of incidence to the cell layer are also being built [5].…”

Section: Introductionmentioning

confidence: 99%

Radiobiological Research and Dosimetry Using a Flat Alpha Source

Szefliński¹,

Filipek²,

Gotlib³

et al. 2020

RAP 2019 Conference Proceedings

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“…Log-phase cells were seeded onto 3.5 cm diameter culture dishes with 6.3 μm of Mylar ® foil base, 24 hours before irradiation. Confluent culture cells were exposed to a monoenergetic 210 Po source developed by Szabó et al (2002) and characterized by emitting 5.297 MeV α-particles with an average LET of 156 keV/μm (Belchior et al, 2010) and a dose rate of 10 mGy/min. During irradiation, part of the culture dish was shielded using 25 μm of aluminum, and in the unshielded area the cells were irradiated with the average cellular doses of 5, 10, 50 and 100 mGy ( Figure!1).…”

Section: Cell Culture and 210 Po Irradiationmentioning

confidence: 99%

Does the Number of Irradiated Cells Influence the Spatial Distribution of Bystander Effects?

Belchior

Balásházy

Gil³

et al. 2014

Dose-Response

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There is growing evidence that the radiation effects at low doses are not adequately described by a simple linear extrapolation from high doses, due, among others, to bystander effects. Though several studies have been published on this topic, the explanation of the mechanisms describing the bystander effects remains unclear. This study aims at understanding how the bystander signals are or can be propagated in the cell culture, namely if the number of irradiated cells influences the bystander response. An A549 cell line was exposed to several doses of α-particles, being the bystander response quantified in two non-irradiated areas. The radius of irradiated areas differs by a factor of 2, and the non-irradiated areas were optimally designed to have the same number of cells. Our results show evidence for bystander effects occurring in cells far away from the irradiated ones, meaning that bystander signals can easily spread throughout the cell culture. Additionally, our study highlights that the damage caused by radiation on the surrounding of irradiated areas could be different according to the number of irradiated cells, i.e., for the same dose value; the overall cellular damage could be different.

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“…The distance between the source and the detector can be adjusted and by this way the alpha energy can be changed. The source here is 210 Po, deposited onto a silver plate and placed into a copper holder and covered by a collimator which ensures the perpendicular incidence on the detector (Szabo et al, 2002). A standard series of track diameters on the dependence of alpha energy and for different etching conditions can be experimentally obtained, which helps to check whether an etching is performed as required or planned.…”

Section: Control Of Etching With An Alpha Irradiation Facilitymentioning

confidence: 99%

“…Garty et al, (2005)proposed a novel microbeam facility for biological irradiation studies. Dosimetric characterisation of a simple irradiation device is given by Szabo et al, (2002). A calibration protocol for boron bio-distribution by autoradiography has been developed by Skvarc et al, (2002).…”

Section: Radioisotopes -Applications In Bio-medical Sciencementioning

confidence: 99%