In vitro challenge using thymoquinone on (HepG2) cell line

Hassan, Sohair A.; Ahmed, Wafaa; Galeb, Fayek M.; El-Taweel, M.

doi:10.1016/j.toxlet.2009.06.400

Cited by 24 publications

(25 citation statements)

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“…Caspase-3 has been found to be necessary for typical role in apoptosis, where it is responsible for chromatin condensation and DNA fragmentation [31] . In addition, we observed that fucoidan treatment enhanced intracellular ROS levels in dose-dependent manner, suggesting that induction of oxidative stress was an important event in the cell death induced by fucoidan [32] .…”

Section: Discussionmentioning

confidence: 77%

Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Banafa¹,

Roshan²,

Liu³

et al. 2014

IOSRJPBS

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Summary: Previous studies have shown fucoidan could induce apoptosis in breast cancers, while the detailed mechanisms need to be investigated.Results of this study illustrated that number of viable MDA-MB-231 cells was decreased by fucoidan with a dose-dependent manner, while the fucoidan did not affect the viable epithelial cells number of normal human mammary (MCF10A).. After incubation with fucoidan, several apoptotic events such as chromatin condensation and increase of the population of G2/M phase were observed in MDA-MB-231 cells. RT-PCR and Western blot results showed that the activation of caspase cascade such caspase-3, caspase-8 and caspase-9, as well as the expression of XIAP (X-linked inhibitor of apoptosis protein), was involved in the apoptosis process. Besides that, reactive oxygen species(ROS) production was increased after fucoidan treatment. These results indicated that fucoidan could induce the apoptotic cell death through modulation of XIAP and caspases activation in MDA-MB-231 cells and could also induce the generation of ROS.

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Section: Discussionmentioning

confidence: 77%

Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Banafa¹,

Roshan²,

Liu³

et al. 2014

IOSRJPBS

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“…Thymoquinone was also reported to possess chemotherapeutic effects on SW-626 colon cancer cells which was comparable to 5-fluorouracil (Norwood et al, 2006;. Moreover, thymoquinone showed promising anti-cancer activity against hepatocellular carcinoma by the inhibition of HepG2 cells in a dose-dependent manner (Ahmed et al, 2008;Hassan et al, 2008). Recently, thymoquinone was shown to inhibit the proliferation of a panel of human cancer cell lines (Caco-2, HCT-116, LoVo, DLD-1 and HT-29), with no cytotoxicity to normal human intestinal cells (FHs74Int); (El-Najjar et al, 2010).…”

Section: Thymoquinone and Related Compoundsmentioning

confidence: 99%

Anticancer Activity of Nigella sativa (Black Seed) — A Review

2011

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Nigella sativa (N. sativa) seed has been an important nutritional flavoring agent and natural remedy for many ailments for centuries in ancient systems of medicine, e.g. Unani, Ayurveda, Chinese and Arabic Medicines. Many active components have been isolated from N. sativa, including thymoquinone, thymohydroquinone, dithymoquinone, thymol, carvacrol, nigellimine-N-oxide, nigellicine, nigellidine and alpha-hederin. In addition, quite a few pharmacological effects of N. sativa seed, its oil, various extracts and active components have been identified to include immune stimulation, anti-inflammation, hypoglycemic, antihypertensive, antiasthmatic, antimicrobial, antiparasitic, antioxidant and anticancer effects. Only a few authors have reviewed the medicinal properties of N. sativa and given some description of the anticancer effects. A literature search has revealed that a lot more studies have been recently carried out related to the anticancer activities of N. sativa and some of its active compounds, such as thymoquinone and alpha-hederin. Acute and chronic toxicity studies have recently confirmed the safety of N. sativa oil and its most abundant active component, thymoquinone, particularly when given orally. The present work is aimed at summarizing the extremely valuable work done by various investigators on the effects of N. sativa seed, its extracts and active principles against cancer. Those related to the underlying mechanism of action, derivatives of thymoquinone, nano thymoquinone and combinations of thymoquinone with the currently used cytotoxic drugs are of particular interest. We hope this review will encourage interested researchers to conduct further preclinical and clinical studies to evaluate the anticancer activities of N. sativa, its active constituents and their derivatives.

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“…There is an emergent interest to study the therapeutic potential of Ns crude extract or pure compound in cancer therapy. Previous research reported about the cytotoxicity effects of TQ on several cancer cells such as human colon cancer cell [7], human pancreatic cancer cell [8], hepatocellular carcinoma [9] and etc. Zingiber zerumbet (Zz) or locally known as 'Lempoyang' is a wild ginger that belongs to Zingiberaceae family [10].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Study of Nigella Sativa and Zingiber zerumbet Extracts, Thymoquinone and Zerumbone Isolated on Human Myeloid Leukemia (HL60) Cell

Norfazlina¹,

Zuraina²,

N.F³

et al. 2014

TOPROCJ

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Abstract:The cytotoxicity effects of Nigella sativa (Ns) and Zingiber zerumbet (Zz) extracts on acute Human myeloid leukemia cell line (HL60) are reported. The HL60 cell line was purchased from the American Tissue Culture Company (ATCC) was treated with petroleum ether (PE) and aqueous (AQ) extracts of Ns and Zz extracts of ethanol (ET) and Hexane (HEX). In an attempt to explain cytotoxicity effects of Ns and Zz extracts by 3-[4,5-dimethylthizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, we examined the effects of Doxorubin (Dox) on their sensitivity as anticancer drugs which was assigns as a positive control in this experiment. Different plant species has different component as bioactive compound. The different activity is present by the different compound. The cytotoxicity effects of plant species are differed based on the presence of their bioactive compound. Therefore, this study was carried out to compare the cytotoxicity effects of Nigella sativa (Ns) and Zingiber zerumbet (Zz) extracts on HL60 cell lines since both of these plants are from different species. Furthermore, this study was also to compare the cytotoxicity effect of plant extract and its compound which are Ns extract and thymoquinone, Zz extract and zerumbone. In this study, Hexane extract of Zz significantly dropped off the percentage of cell viability of HL60 cell by giving the lowest IC 50 value which is 63.72ug/ml (p<0.05). Hexane extracts is more effective solvent compared to ethanol that gave IC 50 value of 345.3ug/ml. Ns extract is less effective on HL60 cell lines that gives the highest IC 50 value which are 654ug/ml for PE and greater than 1000ug/ml for AQ. It is concluded that the cytotoxicity effects of Zz is more proficient as compared to Ns in this cell. HEX extract is more appropriate solvent for the Zz extraction that gave the lowest IC 50 as compared to ET. In addition, PE extract shows the characteristic of more appropriate solvent for Ns extraction compared to AQ solvent since gives the lowest IC 50 value. On the other hand, both of plant species showed apoptosis as mode of cell death.

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In vitro challenge using thymoquinone on (HepG2) cell line

Cited by 24 publications

References 0 publications

Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Fucoidan induces Apoptosis in MDA-MB-231 Cells by Activating Caspase Cascade and Down-regulating XIAP

Anticancer Activity of Nigella sativa (Black Seed) — A Review

Cytotoxicity Study of Nigella Sativa and Zingiber zerumbet Extracts, Thymoquinone and Zerumbone Isolated on Human Myeloid Leukemia (HL60) Cell

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