In vitro characterization and clinical use of platelet‐rich plasma‐derived Endoret‐Gel as an autologous treatment for atrophic scars

Abstract

Background Normal healing process becomes severely dysregulated in pathophysiological conditions such as inflammation, infection or underlaying diseases. These scenarios hamper the standard healing pattern and dermal fibrotic tissue develops. Objective In the present study a novel three‐dimensional formulation (Endoret‐Gel) based on plasma rich in growth factors technology (Endoret‐PRGF) has been assessed for atrophic scar management. Materials and methods Microstructure analysis, growth factor content, and projection capacity of both formulations (Endoret‐Gel and Endoret‐PRGF) was assessed. Additionally, a clinical evaluation of Endoret‐Gel treatment was also performed in a case of an extense atrophic scar. Results Endoret‐Gel presented high molecular weight plasmatic proteins that formed solid thermal aggregates enclosed by a stable fibrin network. This formulation has a higher cutaneous projection capacity compared with Endoret‐PRGF. Both formulations presented a high load of bioactive proteins such as EGF, PDGF‐AB, and IGF‐I being higher in liquid Endoret‐PRGF. Clinical results evidenced that infiltrations of Endoret‐Gel derived in an early volumetric disposal that was maintained for several months. The treatment provided and immediate soft tissue augmentation and scar amelioration effect that was translated into a noticeable clinical improvement of the injury. No side effects or adverse events were reported during ten‐month follow‐up period. Conclusion These preliminary findings suggest that Endoret‐Gel may act not only as a temporary volumizer but also as soft tissue stimulator that might be used as a monotherapy for scar management.

Eficácia clínica do Concentrado- Plasma Rico em Fibrina (C-PRF) associado à vitamina C como bioestimulador

“…A centrifugação do C-PRF demonstrou histologicamente que, além de apresentar grande concentração celular, há viabilidade na sua separação de forma mais adequada quando for empregado o protocolo de plasma gel, pois as células estão localizadas dentro da camada leucocitária diretamente acima da camada de hemácias (0,3 a 0,5 ml) 17 . Atualmente, os métodos conhecidos têm usado apenas o gel de albumina, que é completamente desprovido de células e fatores de crescimento em função das altas temperaturas de processamento [18][19] .…”