In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols

Ramirez-Mares, Marco Vinicio; Chandra, Sonia; Mejía, Elvira González de

doi:10.1016/j.mrfmmm.2004.03.002

Cited by 117 publications

(70 citation statements)

References 45 publications

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“…The extraction process used in this work made it possible to obtain extracts with a satisfactory content of phenolic compounds even though we did not use any extraneous antioxidantsto protect the samples from deterioration. Polyphenolic compounds found in mate differ significantly from green tea because mate leaves contain high concentrations of chlorogenic acid and no catechins [23]. Literature shows that I. paraguariensis is especially rich in chlorogenic acids [12,24], and in our work we proved that the selected plant belonging to the genus Ilex, possess a similar antioxidant pattern with high amounts of chlorogenic acid as well as its isomers and similar in chemical character diesters.…”

Section: Discussionsupporting

confidence: 62%

Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.

Zwyrzykowska

Kupczyński

Jarosz³

et al. 2015

Open Chemistry

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Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp. DOI: 10.1515/chem-2015-0142 received February 15, 2015 accepted October 1, 2015. Abstract: Natural compounds are an important source of desired biological activity which help to improve nutritional status, enhance productivity and bring many health benefits. The leaves of the Ilex paraguariensis (Aquifoliaceae) are used for preparing a beverage known as yerba mate and represent a proven source of natural polyphenols which are known to foster biological activity with the emphasis on antioxidant properties. In present work we focused on the polyphenolic content of air-dried leaves of Ilex aquifolium L., Ilex aquifolium 'Argentea Mariginata', Ilex meserveae 'Blue Angel', and a commercially available mate as the reference product. Liquid chromatography combined with mass spectrometry (HPLC and LC-MS) and thin layer chromatography (TLC), were used to establish polyphenolic substances content in aqueous methanolic extracts obtained from the biological matter. Up to 20 polyphenolic compounds were identified in the extracts, including rutin, quinic acid and its caffeoyl esters, i.e. chlorogenic acid and its isomers as well as dicaffeoyl derivatives. We took chlorogenic acid and rutin as reference compounds to quantify their levels in the extracts. It was determined that in all tested plants, high levels of these antioxidants were present. This led us to the conclusion that their leaves might serve as valuable food additives.

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Section: Discussionsupporting

confidence: 62%

Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.

Zwyrzykowska

Kupczyński

Jarosz³

et al. 2015

Open Chemistry

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“…Several studies on yerba maté have reported the presence of xanthines such as caffeine and theobromine, saponines, and several phenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives (FILIP et al, 2000;SCHINELLA et al, 2000;RAMIREZ-MARES;BORTOLUZZI et al, 2006;GUGLIUCCI et al, 1996); Dudonne et al (2009) reported 200 mg gallic acid equivalents per g of powder extract and Bravo et al (2007) reported 45 mg caffeoyquinic acids per g of dry samples. It has also been reported that yerba maté extracts have an in vitro antioxidant capacity (AOC), which is due to the presence of polyphenolic compounds that have an antioxidant capacity equal to or higher than that of ascorbic acid and vitamin E (FILIP et al, 2000;SCHINELLA et al, 2000;RAMIREZ-MARES;GUGLIUCCI et al, 1996;GONZALEZ DE MEJIA et al, 2005).…”

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confidence: 99%

“…It has also been reported that yerba maté extracts have an in vitro antioxidant capacity (AOC), which is due to the presence of polyphenolic compounds that have an antioxidant capacity equal to or higher than that of ascorbic acid and vitamin E (FILIP et al, 2000;SCHINELLA et al, 2000;RAMIREZ-MARES;GUGLIUCCI et al, 1996;GONZALEZ DE MEJIA et al, 2005). Dudonné et al (2009) placed yerba maté aqueous extracts between the fifth plant extracts with higher antioxidant activity among 30 selected plants analyzed.…”

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confidence: 99%

A novel procedure to measure the antioxidant capacity of Yerba maté extracts

et al. 2012

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Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 µL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 µM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.

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“…Em estudos in vitro, o extrato de erva-mate contribuiu na prevenção do câncer. 3,4 Estudos in vivo com infusão de erva-mate demonstraram a capacidade de inibição da auto-oxidação do LDL (lipoproteína de baixa densidade), 5 a eficácia no tratamento contra obesidade 6 e ação cardioprotetora. 7 Os benefícios atribuídos ao consumo da infusão de erva-mate estão relacionados aos compostos fenólicos, metabólitos secundários produzidos pelas plantas, 8 que atuam como antioxidantes, 9 sendo oxidados em preferência a outros constituintes do alimento ou componentes celulares e tecidos.…”

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Determinação de compostos fenólicos por cromatografia líquida de alta eficiência isocrática durante estacionamento da erva-mate

Dutra¹,

Ribani²,

Ribani³

2010

Quím. Nova

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Recebido em 14/2/09; aceito em 9/6/09; publicado na web em 13/11/09 DETERMINATION OF PHENOLIC COMPOUNDS BY ISOCRATIC HIGH PERFORMANCE LIQUID CHROMATOGRAFIC METHOD DURING STORAGE OF YERBA-MATE. Different phenolic compound, 5-caffeoylquinic acid (5-CQA), caffeic acid (AC) and rutin (Ru) contents of yerba-mate (Ilex paraguariensis) Brazilian samples of 06 different regions of São Mateus -Paraná, during natural and accelerated industrial storage, were evaluated. For quantification, a reverse phase HPLC isocratic method was developed and validated using methanol:water (35:65 v/v) acidified with 0.5% acetic acid as mobile phase and a photodiode array detector. The six sample global average contents were (34.90 and 36.10 mg g ) for AC and (7.12 and 7.18 mg g -1 ) for Ru, respectively, for the natural and accelerated storage systems. The results showed that the 5-CQA and Ru content are kept constant during the storage while AC content increase only during accelerated storage.Keywords: HPLC analysis; phenolic compounds; Ilex paraguariensis. INTRODUÇÃOA erva-mate (Ilex paraguariensis Saint Hilaire) é uma espécie nativa brasileira, destacando-se como fonte econômica, social e ecológica para a região sul do Brasil, norte e leste da Argentina e Paraguai, sendo consumida principalmente na forma de chimarrão. 1,2Vários efeitos benéficos à saúde humana têm sido atribuídos ao consumo da erva-mate. Em estudos in vitro, o extrato de erva-mate contribuiu na prevenção do câncer. 3,4 Estudos in vivo com infusão de erva-mate demonstraram a capacidade de inibição da auto-oxidação do LDL (lipoproteína de baixa densidade), 5 a eficácia no tratamento contra obesidade 6 e ação cardioprotetora. 7Os benefícios atribuídos ao consumo da infusão de erva-mate estão relacionados aos compostos fenólicos, metabólitos secundários produzidos pelas plantas, 8 que atuam como antioxidantes, 9 sendo oxidados em preferência a outros constituintes do alimento ou componentes celulares e tecidos.10 Estão presentes na erva-mate, dentre outros compostos, a rutina (Ru), um flavonol pertencente à classe dos flavonoides e os ácidos 5-cafeoilquínico (5-CQA) e cafeico (AC), pertencente aos ácidos fenólicos. 11,12 Filip et al. 13 correlacionaram a atividade antioxidante da infusão de erva-mate com conteúdo de rutina, quercetina, kampferol e derivados cafeoilquínicos desta.De acordo com a literatura, 14-18 os teores em base seca de Ru, AC e 5-CQA encontrados na erva-mate, variam de 0,60 a 13,00 mg g A cromatografia líquida de alta eficiência (CLAE) tem sido a técnica mais empregada na análise de compostos fenólicos em ervamate, utilizando principalmente gradiente da fase móvel para separação de diferentes classes de compostos, como os flavonoides e ácidos hidroxicinâmicos, principais componentes presentes na erva-mate. 7,14,22 Muitos laboratórios de instituições disponibilizam sistemas CLAE equipados apenas com bomba simples, de menor custo, e empregando eluição isocrática, mantendo constante o fluxo da fase móvel na coluna, por ser um sistema de fácil operação e com...

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In vitro chemopreventive activity of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts and selected polyphenols

Cited by 117 publications

References 45 publications

Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.

Qualitative and quantitative analysis of polyphenolic compounds in Ilex Sp.

A novel procedure to measure the antioxidant capacity of Yerba maté extracts

Determinação de compostos fenólicos por cromatografia líquida de alta eficiência isocrática durante estacionamento da erva-mate

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