In vitro clonal multiplication of 4-year-old plants of the bamboo,Dendrocalamus longispathus kurz

Saxena, Sanjay; Bhojwani, Sant S.

doi:10.1007/bf02632285

Cited by 55 publications

(52 citation statements)

References 18 publications

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“…The performance of IAA and IBA was tested where IBA proved to be better than IAA. This is in support to the earlier report of Saxena and Bhojwani (1993) in Dendrocalamus longispathus. Notwithstanding the fact, IAA was proved to be inactive in D. aspar root initiation and elongation.…”

Section: Resultssupporting

confidence: 93%

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A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field

Banerjee

Gantait

Pramanik

2011

Physiol Mol Biol Plants

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An accelerated protocol for large-scale propagation of Dendrocalamus asper, an edible bamboo, has been described. Seven axillary shoots were induced in vitro from each excised tender node (15-20 mm in length) containing single axillary bud when nodal segments were inoculated in semisolid Murashige and Skoog (MS) medium fortified with 5 mg/l 6-benzylaminopurine (BAP). Maximum multiple shoot formation (14) was observed when in vitro generated axillary shoots were transferred to liquid MS medium containing 5 mg/l BAP and 40 mg/l adenine sulphate. A maximum of 93.33 % shoots were effectively rooted when transferred to liquid MS medium supplemented with 1 mg/l indole-3-biutyric acid (IBA). A simple acclimatization procedure of 55 days, primarily in cocopeat for 20 days and finally in a blend of sand, soil and farm yard manure (1:1:1 v/v), ensured a very high survival rate within next 35 days. After acclimatization, rooted plantlets were further multiplied by splitting of rhizomes, formed in vivo within 90 days of growth. After 90 and 180 days of acclimatization, plants were successfully transferred to the field and maintained in an unirrigated condition with the initial supplementation of farm yard manure @ 10 kg/pit; where around 85 % survivability with 25 culms per bush attaining an average height of 4.5 m was recorded up to four years.

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Section: Resultssupporting

confidence: 93%

“…Nevertheless, cuttings are bulky, tricky to handle and have been found to grow very slowly (Hassan 1980;Rao et al 1985). Further year round, vegetative propagation is complicated due to seasonal specificity of material (Saxena and Bhojwani 1993). Consequently, the species, being exotic to India, authentic source material is scarce.…”

Section: Introductionmentioning

confidence: 99%

A two step method for accelerated mass propagation of Dendrocalamus asper and their evaluation in field

Banerjee

Gantait

Pramanik

2011

Physiol Mol Biol Plants

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“…Nadgauda et al (1990) and Rajapakse, (1992) induced shoot proliferation in the medium supplemented with coconut water in seedling derived culture of B. bambos and D. brandisii and in D. giganteus respectively, for which in vitro flower were also induced. Saxena and Bhojwani, (1993) reported to use 10% coconut milk (CM) as additive for better shoot proliferation in D. giganteus. Ramanayake et al (2001) reported that a high level of sucrose (4%) adversely affected the shoot multiplication in D. giganteus.…”

Section: Resultsmentioning

confidence: 99%

“…The offsets used are bulky and heavy. Clumps are not always available due to seasonal specificity, so that large scale vegetative propagation is impossible (Saxena and Bhojwani, 1993). Because of the difficulties with these traditional propagation methods, in vitro methods of propagation (micropropagation) provides an alternative to meet the demand of the market, which allow the rapid multiplication of disease-free and true to type selected clones.…”

Section: Introductionmentioning

confidence: 99%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

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“…It is a common practice to transfer shoots from a high strength media to less concentrated solutions to induce rooting (Bopana and Saxena 2008). In many species such as Dendrocalamus longispathus (Saxena and Bhojwani 1993), Adhatoda vaisca (Azad et al 2003), Phellodendron amurense (Yang et al 2011, Azad et al 2005a, and Curcuma caesia rooting frequency was higher when shoots were rooted on low strength MS. The regenerated plantlets were gradually acclimated and successfully established in the soil under natural conditions (Fig.…”

Section: Resultsmentioning

confidence: 99%