In vitro collection of cultivars and wild forms of Berberis spp for cryobank creation

Ромаданова, Н.В.; Mishustina, S.A.; Karasholakova, Lazzat; Reed, Barbara M.; Кушнаренко, С. В.

doi:10.1016/j.jbiotec.2016.05.153

Cited by 2 publications

(2 citation statements)

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“…An information system (database Biodiversity of flora in Kazakhstan) has been created (https://kazflora.kz/), according to the genetic and botanical description of endemic, rare, endangered, and economically useful species of the flora of Kazakhstan. This information system was created for cataloging information, conservation, and rational use of plant genetic resources in Kazakhstan [159,162].…”

Section: Biobanks Of Flora and Fauna In Kazakhstanmentioning

confidence: 99%

Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna

Dairov,

Mukanov,

Khapilina

et al. 2024

Eurasian Journal of Applied Biotechnology

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To date, human actions have significantly changed 75% of the Earth's surface. This has resulted in an unprecedented increase in the rate of biodiversity loss in human history. According to the International Union for Conservation of Nature and Natural Resources, over the past 500 years, as a result of direct or indirect influence of the anthropogenic factor, 774 animal species and 123 plant species have become extinct. To prevent further loss of biodiversity, in the context of the ongoing «crisis of biodiversity», all over the world, including Kazakhstan, there is a need for reliable conservation of the available genetic resources of plants and animals, primarily rare and endangered species. In this regard, specialized biobanks of organized collections of living biomaterials are successfully used worldwide for the conservation and restoration of populations of rare and endangered species of flora and fauna. The article presents information about some of the largest biobanks and their importance in solving this global problem. The experience of scientific centers of Kazakhstan in the field of biobanking of biomaterials of wild flora and fauna, including rare and endangered species, is given, and the results of the National Center for Biotechnology in this area are summarized.

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Section: Biobanks Of Flora and Fauna In Kazakhstanmentioning

confidence: 99%

Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna

Dairov,

Mukanov,

Khapilina

et al. 2024

Eurasian Journal of Applied Biotechnology

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“…(Khromov and Cherednichenko 2022). Biotechnological methods are also being developed to obtain callus tissues for further micropropagation or cryopreservation (Romadanova et al 2015;Efferth 2019). Despite this, now the process of somatic embryogenesis and the induction of callus tissue, as well as other issues of P. ledebouriana cultivation, are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Callus induction with 6-BAP and IBA as a way to preserve Prunus ledebouriana (Rosaceae), and endemic plant of Altai and Tarbagatai, East Kazakhstan

Orazov

MYRZAGALIYEVA²,

MUKHITDINOV³

et al. 2022

Biodiversitas

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Abstract. Orazov A, Myrzagaliyeva A, Mukhitdinov N, Tustubayeva S. 2022. Callus induction with 6-BAP and IBA as a way to preserve Prunus ledebouriana (Rosaceae), and endemic plant of Altai and Tarbagatai, East Kazakhstan. Biodiversitas 23: 3178-3184. This article presents the results of the study of in vitro conditions for the cultivation and induction of callus tissues of a rare plant species Prunus ledebouriana(Schlecht.) Y.Y.Yao (Ledebour's almond), endemic plant to East Kazakhstan. For the first time, protocol and optimal composition of Murashige & Skoog (MS) were developed for obtaining calendemiclus tissues. The main indicator of successful introduction was the frequency of callus tissue formation in vitro. Two types of explants were used: the whole embryos and embryos with a removed root part. The removal of the root part inhibited the organogenesis of the embryonic root and the first shoot, which allowed the accumulation of nutrients for the growth of the callus mass and increased the frequency by 7.79±0.46%. Various exogenous phytohormones such as Kinetin, 6-BAP, GA, IBA were added to the MS culture medium. A decrease in Kinetin concentrations of 0.04 mg/L resulted in a lower intensity, but a higher frequency of formation (72.00±5.66%) and mass gain. The average concentration up to 0.5 mg/L of exogenous phytoregulators, such as GA and 6-BAP, resulted in frequency from 65.38±5.44% to 72.00±5.66%, the concentration of IBA up to 1 mg/L increased the frequency from 46.00±2.83 to 72.00±5.66%. The protocol for obtaining callus from an embryonic explant and the composition of the optimal nutrient medium for the induction of callusogenesis made it possible to obtain P. ledebouriana meristem tissues for further use in the development of callus cryopreservation technology.

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In vitro collection of cultivars and wild forms of Berberis spp for cryobank creation

Cited by 2 publications

References 0 publications

Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna

Biobanks as a way to conserve biodiversity in the context of the continuing decline in the species diversity of wild flora and fauna

Callus induction with 6-BAP and IBA as a way to preserve Prunus ledebouriana (Rosaceae), and endemic plant of Altai and Tarbagatai, East Kazakhstan

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