In vitro conservation and production of vigorous and desiccate tolerant synthetic seeds in Stevia rebaudiana

Abstract

Artificial (Synthetic) seed production along with microprpagation can solve many problems with regeneration of Stevia rebaudiana, which produce tiny and non-viable seeds. Shoot tips and axillary buds nodal segments were used from in vitro cultures for encapsulation. The explants were taken from green houses and tissue culture laboratory of AgriBiotech Research Farms, Lahore, Pakistan. Different concentrations of sodium alginate and calcium chloride affect the shape and germination of artificial seeds. A gelling matrix of 3% sodium alginate and 100 mM calcium chloride was found most suitable for the formation of firm, clear and isodiametric ideal beads. No effect of the duration of sodium alginate treatment on bead formation was observed. However, timing of CaCl 2 treatment proved to be crucial with 100 mM CaCl 2, treatment time of 15 min was suitable for isodiametric bead formation. The synthetic seeds pre-treated with KN0 3 germinated earlier than seeds without pretreatment with KNO 3. Root formation took place after 15 days of shoot formation without addition of any auxin. In liquid medium, the frequency of conversion was high. Frequency to plantlet conversion of synthetic seeds decreased gradually as the storage duration at 4°C increased. After 15, 30, 45 and 60 days of storage, frequencies to plantlet conversion from encapsulated and non-encapsulated segments were 86,

In vitro regeneration of Aristolochia tagala and production of artificial seeds

“…Both sodium alginate and CaCl 2 play an important role in capsule quality (Singh et al 2010). Similar reports of a maximum frequency of plantlet regeneration with 3 % alginate were given by Gozukirmizi (2003) in Paulownia elongate, Mohanraj et al (2009) in Coelogyne breviscapa, and Ali et al (2012) in Stevia rebaudiana. It was observed that the use of liquid MS medium for preparing the gel matrix was beneficial in increasing the shoot emergence.…”

Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology

“…Different compounds can be used for synthetic seed desiccation treatment [80]. In a study on Stevia rebaudiana [83] and sugarcane [84], KNO 3 was applied before synthetic seed germination. Usually, treatment with sucrose permits low moisture content of the explant through dewatering due to low water potential in the external environment.…”