In Vitro Conservation Protocols for Some Threatened Medicinal-Plant

Shahzad, Anwar; Shaheen, Arjumend

doi:10.1007/978-94-007-6603-7_13

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…In vitro regeneration techniques offer an efficient method for propagation of threatened medicinal plants (Jha et al 1984;El Grari and Backhaus 1987;Stojakowska 1993;McCartan and Van Staden 1999;Shahzad and Shaheen 2013;Yanthan et al 2017). In vitro regeneration via direct and indirect organogenesis through semi-solid and liquid culture and synthetic seeds have been identified as a potential resource for ex situ conservation and commercial applications, continuous production of plants without any seasonal limitations, in vitro gene banking and exchange of germplasm across international borders.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant

2017

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Efficient in vitro propagation systems via organogenesis and synthetic seeds were developed for the first time for conservation and commercial propagation from leaf or longitudinal thin cell layer (lTCL) leaf or shoot-tip explants of . Various plant growth regulators and phloroglucinol were used in semi-solid and liquid Murashige and Skoog (MS) medium to establish multiplication of shoots and roots for in vitro regeneration. Of the various treatments, the highest number of shoots (17.4 per lTCL leaf explant) was obtained on liquid MS medium supplemented with 10 µM-Topolin (T) and 2 µM benzyladenine followed by transferal to semi-solid MS media. The shoot tips were encapsulated with liquid MS medium plus 3% (w/v) sodium alginate and 100 mM calcium chloride. Adventitious shoot regeneration (91.0%; 12.6 shoots per synthetic seed) of synthetic seeds was achieved on semi-solid MS medium supplemented with 10 µM T and 2 µM naphthaleneacetic acid (NAA) after 15 days of storage in darkness at 25 ± 2 °C. Regenerated shoots rooted (9.8 roots per shoot; 6.5 cm long) efficiently when transferred to 5 µM indole-3-butyric acid and 2.5 µM NAA. All the plantlets were successfully acclimatized (100%) in a vermiculite:soil (1:1 v/v) mixture in the greenhouse.

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Section: Introductionmentioning

confidence: 99%

In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant

2017

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“…Further, A. wallichii has enormous olericulture importance and consider as high cash value nutraceutical and medicinal plant (Paudyal et al 2021). Moreover, A. wallichii was considered as threatened plant of Nepal and a micropropagation method by shoot culture for production of plantlets suitable for field culture was established earlier (Wawrosch et al 2001, Shahzad & Shaheen 2013. Additionally, protected cultivation, information and training to farmers about A. wallichii may be useful for maximum utilization and exploration the economic potential in terms of health benefits in near future.…”

Section: Biodiversity and Conservation Statusmentioning

confidence: 99%

Ethnomedicinal and traditional application of Allium wallichii Kunth (Himalayan Onion): An unexplored and underutilized nutraceutical plant foods from Himalayan regions.

Rana¹,

Sethiya

Duseja³

et al. 2022

Ethnobot. Res. App.

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Background: Allium wallichii Kunth (Family: Amaryllidaceae), commonly known as "Himalayan onion" is well recorded as popular ethnomedicine for medicinal and nutritional uses by different people and communities native to Bhutan, India, Myanmar, Nepal and Southwestern China.Methods: Despite the wide ethnomedicinal and pharmacological studies on A. wallichii, there are no concise elaborated article comprising reviews of published literature. So, herein we designed this review article to discuss the potential health benefit in both traditional ethnopharmacology and modern pharmacology. To achieve this exhaustive literature searches using A. wallichii as keywords for screening of relevant information online databases such as Google Scholar, PubMed, Science Direct, SciELO, Scopus and SpringerLink were performed.Results: Only few published studies relevant to our objective was found and presented under different section of the current work. It was observed that different parts of A. wallichii was recorded in different regions of the Asia for health, economical and nutraceutical benefits such as dysentery, cholera, cold, cough, blood cholesterol levels, itching, to remove maggots from wounds, leech remover, antidote, cut, wounds (finger and toe infections), gastric problems, bile complaints, moth repellent, body ache, sinusitis, carminative, dizziness, mumps, hypertension, intestinal pain, liver diseases, indigestion for children, high altitude sickness, condiment for curries, pickles, soup and vegetable source for cash income. Major bioactive phytochemicals such as 1,2 bis (methylthio) ethene, diosgenin, 2,4 dimethyl thiophene, tigogenin, dimethyl disulfide and trisulfide were reported. However, in modern pharmacology anti-microbial, antioxidant, and anti-cancer activities was established.Conclusion: It was concluded that A. wallichii need special attention for protected cultivation and conservation in near future for maximum output and utilization are required. Moreover, A. wallichii could be considered as an excellent source of safe and effective medicinal and nutritional herbal remedies for human and animal consumption after only careful investigation.

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In Vitro Conservation Protocols for Some Threatened Medicinal-Plant

Cited by 2 publications

References 23 publications

In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant

In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant

Ethnomedicinal and traditional application of Allium wallichii Kunth (Himalayan Onion): An unexplored and underutilized nutraceutical plant foods from Himalayan regions.

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