In Vitro Culture and Characterization of Testis-Derived Cells from Clarias magur (Hamilton, 1822)

Singh, Neha; Murali, S.; Kushwaha, Basdeo; Srivastava, Jaya; Srivastava, Shreya; Mishra, Aashwin; Kumar, Ravindra

doi:10.1007/s40011-018-1020-y

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…() demonstrated that Indian Catfish Fin (ICF), the cell line developed from Clarias batrachus , grew well at at 28°C and 15% FBS. Testis‐derived cells of Clarias magur (previous name C. batrachus ) (Singh et al ., ), L‐15 media with 20% FBS was found suitable, and culture conditions between 25 and 28°C supported cell growth. The caudal fin has a natural regeneration capacity (Akimenko et al ., ) accounting for a high in vitro potential of cell proliferation (Santos‐Ruiz et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…A number of cell lines have been developed and characterized from clariid fish, including the G1B cell line from the gill tissue of C. batrachus (Noga & Hartmann, ), the catfish kidney (CFK) cell line from C. batrachus (Lio‐Po et al ., ), the ICF cell line derived from the fin tissue of walking catfish, C. batrachus (Babu et al ., ), and the testis‐originated cell line from C. magur (previously C. batrachus ) (Singh et al ., ) for the isolation of viruses affecting catfish and analysing pesticide toxicity. Normally, in primary cell culture, cell populations experience a selection process due to factors such as damage to the cell during isolation and competition between different cells for nutrients or substrates, and more than one cell type is ultimately present in a cell line (Freshney, ).…”

Section: Discussionmentioning

confidence: 99%

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Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840

Swaminathan

Dharmaratnam

Raja

et al. 2020

Journal of Fish Biology

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We describe a new cell line, Clarias dussumieri fin (ClDuF), from the caudal fin of C. dussumieri using the explant technique followed by cryopreservation. The cryopreserved CiDuF cells were validated for quality and other characteristics. They showed typical epithelial morphology in vitro and epithelial cells outgrew their fibroblast cells after the fifth passage. ClDuF cells had a characteristic sigmoid curve with population doubling in 24 h. Immunotyping of the ClDuF cells against cytokeratin suggested the epithelial lineage. Chromosome analysis showed normal diploid (2n = 50) numbers and the cells did not contain any contamination, including Mycoplasma and other microbes. Partial sequencing of fragments of mitochondrial 16s rRNA and COI genes of ClDuF confirmed that the cell line was initiated from C. dussumieri. Cells at the 10th and 25th passages had more than 80% and 70% viability in the culture, respectively, after 6 months of storage at LN2. These ClDuF cells were morphologically identical to the cells before freezing and the genetic resource of C. dussumieri was preserved. The species‐specific cells can serve as a valuable source for virus isolation, conservation and cloning of somatic cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840

Swaminathan

Dharmaratnam

Raja

et al. 2020

Journal of Fish Biology

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Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

Thangaraj

Narendrakumar

Geetha

et al. 2021

Reviews in Aquaculture

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Cell culture has become an indispensable part of modern research and is considered the best alternatives for different biological research on whole animals as they mimic the host cellular genetic homogeneity and also reduces the variability of in vivo responses. Hence, there has been increasing development of cell lines by different research groups and companies globally. For this reason, to cover the dearth of knowledge of newly developed fish cell lines, we have made a comprehensive update on new fish cell lines established in the past decade (2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018)(2019)(2020) to reflect the number of cell lines now available. This review focuses on the appraisal of the literature and of work done in finfish cell culture around the globe during the last decade and summarizes 280 newly established fish cell lines derived from 20 different tissue types of 44 fish families belonging to 21 different countries raising the total finfish cell lines to 783. The highest number of cell lines developed in the past decade was from the fish of the family Cyprinidae, which are most widely cultured in Asian countries. Also, the review discusses the need of new cell line development, different methods of cell line development and further enumerates the various applications and future prospects for these cell lines which we believe will serve as a referral document for researchers in the field.

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In Vitro Culture and Characterization of Testis-Derived Cells from Clarias magur (Hamilton, 1822)

Cited by 2 publications

References 23 publications

Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840

Establishment and cryopreservation of a cell line derived from caudal fin of endangered catfish Clarias dussumieri Valenciennes, 1840

Comprehensive update on inventory of finfish cell lines developed during the last decade (2010–2020)

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