2016
DOI: 10.1007/978-1-4939-3795-0_4
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In Vitro Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method

Abstract: Summary/Abstract The development of the Drosophila egg chamber encompasses a myriad of diverse germline and somatic events, and as such, the egg chamber has become a widely used and influential developmental model. Advantages of this system include physical accessibility, genetic tractability, and amenability to microscopy and live culturing, the last of which is the focus of this chapter. To provide adequate context, we summarize the structure of the Drosophila ovary and egg chamber, the morphogenetic events … Show more

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Cited by 18 publications
(12 citation statements)
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“…Live imaging was performed as described (Peters and Berg, 2016). Briefly, stage 13 egg chambers were individually dissected from flies in room temperature Schneider’s medium.…”
Section: Methodsmentioning
confidence: 99%
“…Live imaging was performed as described (Peters and Berg, 2016). Briefly, stage 13 egg chambers were individually dissected from flies in room temperature Schneider’s medium.…”
Section: Methodsmentioning
confidence: 99%
“…No. 81156) using a Kimwipe "blanket" to hold them in place, as described (Peters and Berg 2016b). Images were acquired on a Leica SP8X scanning confocal microscope and processed using Leica software (LAS Version X), FIJI (ImageJ Version 1.51j), and Photoshop (Version 12.1).…”
Section: Live Imagingmentioning
confidence: 99%
“…Each eggshell is produced by an individual egg chamber (Figure 1B,C), which consists of 15 germline-derived nurse cells, a single oocyte, and a surrounding follicular epithelium. The egg chamber is a self-contained unit that can easily be cultured in vitro (Cliffe et al, 2007; Dorman et al, 2004; Manning and Starz-Gaiano, 2015; Peters and Berg, 2016a; Prasad et al, 2007). The development of an egg chamber proceeds through 14 stages (Spradling, 1993); during the stages in which the eggshell is formed (stages 10–14), there is neither cell division nor significant cell death among the follicle cells.…”
Section: Introductionmentioning
confidence: 99%