In Vitro Cytoprotective Effects and Antioxidant Capacity of Phenolic Compounds from the Leaves of Swietenia macrophylla

Abstract

Swietenia macrophylla (mahogany) is a highly valued timber species, whereas the leaves are considered to be waste product. A total of 27 phenolic compounds were identified in aqueous extracts from mahogany leaves by comparing retention times and mass spectra data with those of authentic standards using LC-ESI-MS/MS. Polyphenols play an important role in plants as defense mechanisms against pests and pathogens and have potent antioxidant properties. In terms of health applications, interest has increased considerably in naturally occurring antioxidant sources, since they can retard the progress of many important neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. The antioxidant capacities of two aqueous extracts, M1 (decoction) and M2 (infusion), were measured using TEAC and Folin-Ciocalteau methods. Additionally, M1 was used in order to investigate its potential cytoprotective effects on an in vitro model of neurodegeneration, by using primary OPEN ACCESSMolecules 2015, 20 18778 cerebellar cultures exposed to methyl mercury (MeHg). Under experimental sub-chronic conditions (72 h), concomitant exposure of the same cultures to MeHg and M1 extract resulted in a statistically significant increase in cell viability in all three concentrations tested (10, 50 and 100 μg/mL), strongly suggesting that due to its high content of antioxidant compounds, the M1 extract provides significant cytoprotection against the MeHg-induced in vitro neurotoxicity.

ACUTE ORAL TOXICITY FROM A FRACTION RICH IN PHENOLIC COMPOUNDS FROM THE LEAF EXTRACT OF Swietenia macrophylla KING IN A MURINE MODEL

“…Nevertheless, in other study from leaves aqueous extract of Swetenia were identified nine phenolic acids and 18 flavonoids with antioxidant ability on cell culture, suggesting a cytoprotecting activity (32).…”

Potential Antioxidant Capacities and Neuroprotective Properties of Six Tunisian Medicinal Species

“…All plant extracts, except S.sodomaeum, did not significantly affect cells viabilityat concentration up to 400 µM ( Figure 2a).Indeed, incubation of PC12 cells with 5 µM Aβ (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) , for 24 h decreased the cell viability compared to control to 44% (Figure 2b). In order to evaluate the protective effect of extracts, Aβ was added to PC12 cells simultaneously to the extracts (100, 200 and 400 µg/ml).…”

Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

“…The assay was developed in triplicate and calculations based on a calibration curve obtained with gallic acid. The results were expressed as milligram of gallic acid equivalents (GAE) per g of dry extract [29].…”

“…LC-MS analysis was performed on a XEVO G2-STof Mass Spectrometer (Waters Corp., Milford, MA, USA). The acquisition parameters and chromatographic analysis were described in details previously, with some adjustments, for example, at the time of gradient elution to 20 minutes instead of 10 [29].…”